Recent research on public land production in the broader region highlights similarities and differences between public and private land production. For example, both seem to be located relatively close to rivers and streams, with ~50% canopy cover, and in relatively young stands . However, while we may presume that all production on public lands represents new clearing for production, our results indicate that 32% of farms are on already developed and unforested parcels. Additionally, public lands provide critical refuges for many of the region’s carnivores, which may help explain why public land production appears to overlap more with carnivore habitat than our results for private land production . Perhaps most importantly at a landscape scale, farm size and total extent appear to be much smaller for legacy pathway private land cannabis mapped in this study compared to estimates of public land production practices . Despite the differences between public and private land cannabis production, private land cannabis farming still has characteristics that warrant continued research and planning. Our results suggest that legacy pathway cannabis farming could be compatible and comparable with existing rural land use in Josephine County. In order to ensure this continues to be the case, however, further attention should be given to conservation outreach, policies to support small scale farming, and attention to land use practices on farms, particularly those that may affect carnivores and coho salmon. As the industry continues to expand, policymakers and conservationists need to clarify landscape level strategies to ensure a sustainable future. Care should be taken when interpreting these results, since cannabis agriculture takes many forms and often exhibits regional differences in production practices that may influence its ecological impact . Our study, by nature of our mapping approach, evaluated outdoor production on private lands. We were unable to quantify whether the farms we mapped were illegal or licensed medically or recreationally, nor how many farms we may have missed by farmers effectively concealing their crop. Given our mapped sites included 2,227 farms in 2016 compared to the 43 recreationally licensed locations in 2016 , plant grow trays it is likely that most of the farms we georeferenced were not licensed. If this is the case, the lack of effort to conceal crops is notable.

We suspect because cannabis was pervasive , that enforcement would not have been feasible . Therefore, we were confident that our study accurately quantified the distribution of private-land cannabis production because of the visibility of both licensed and unlicensed farms from aerial imagery. Further, our data likely does not capture all of the cannabis being grown in Josephine County as we were unable to quantify concealed farms on public land or indoor cannabis production. Instead, our study offers critical insights into the ecological consequences of the growing industry in legacy production regions. The overall cultivated area of private land cannabis agriculture at the landscape scale in Josephine County in 2016 appears to be similar to small-scale rural development already occurring regionally. For example, in a county of 4,250 km2 , the total cannabis cultivation area was only 1.34 km2 . This small size is similar to other agricultural production in the county: in 2017, Josephine County produced 2.98 km2 of grapes and 0.48 km2 of vegetables . Cannabis in Josephine County was also considerably smaller in scale than other legacy cannabis-producing regions in Northern California in 2016, where averages ranged from 53-119 plants per site, compared with the median of 21 found in our study . While we do not have comparative research on the ecological effects of other agriculture in the study area, small-scale agriculture in rural areas often creates a landscape mosaic that supports species richness . The ability of small-scale cannabis farming to function like agriculture in other working lands systems, however, requires a deeper understanding of land use practices associated with cannabis production. Specifically, to be ecologically sustainable, small scale private land cannabis farms would need to create a significantly smaller ecological footprint than public land cannabis . Although the area of cultivation for cannabis in Josephine County was small, this study did not evaluate the edge effects of cannabis cultivation, nor take into account other forms of disturbance associated with the sites, such as clearing beyond the cultivated area, road construction, or water storage development. Therefore, the actual overlap and potential ecological effect from cannabis farming in the region is likely to be larger than what was documented in this study. Our understanding of these broad scale impacts would be enhanced in future studies that may be able to assess the fine scale response of wildlife on and surrounding cannabis farms.

While our study does not address direct effects of cannabis production, we did identify spatial relations of cannabis development that could pose unique risks to terrestrial and freshwater ecosystems. We found that cannabis production was clustered in its distribution, which is consistent with research from northern California . Similarly, the proliferation of fences associated with cannabis could be a concern for habitat fragmentation as the industry expands . The overlap results indicate that cannabis may be grown disproportionately in forests and at higher elevations, which suggests cannabis could be associated with greater land clearing than other development on private parcels. However, the forests where cannabis was grown did not appear to be denser or older than comparable parcels. Our results indicate a large overlap of cannabis farms with areas of high projected fisher occupancy. This overlap was greater on cannabis farms than private land generally, but could be due to a higher proportion of cannabis farms located at higher elevations . However, elevation alone doesn’t explain this overlap. Fisher occupancy was projected to be higher on cannabis farms than the areas immediately surrounding them . This suggests that even at fine scales, farms are appearing in areas of potential for high quality habitat for fisher. What this overlap may mean for fisher populations is unclear, given the lack of research on the impacts of private land cannabis production. Private land cannabis has not been documented to have the same negative effects on fishers as public land production, and in particular pesticide and toxicant use appears to be lower on private land farms, according to self-reported farmer surveys . However, anecdotal reports and local news stories raise concerns for these private land farms as well, and many grower organizations have emphasized a need for stronger environmental norms among farmers. Given the remaining uncertainty, these results emphasize the potential need for conservation attention to private land farms as well. Surprisingly, the individual species differences did not add up to differences in overall carnivore richness, which was relatively consistent across the study area. This raises the possibility that the differences in carnivore distributions might be driven by competitive interactions , though finer scale research would be needed to disentangle the drivers of these species distribution patterns in relation to cannabis production. Regarding potential interactions between cannabis production and freshwater ecosystems, the picture was also somewhat mixed. There were a number of farms within 15 m of rivers and streams, but this was not surprising given the high density of rivers and streams in the study area. On average, most farms were only slightly closer to rivers and streams than the surrounding context of all private land parcels. Cannabis was located on average 273 m closer to coho salmon habitat than private parcels overall, 387 m closer to fall run chinook, and 132 m closer to winter run steel head, though the IQR intervals overlap. This proximity to freshwater in Josephine County was also generally closer than observed in other legacy cannabis regions . For example, the proportion of sites in Josephine County within 500 m of coho habitat was more than twice the proportion in northern California . Butsic et al. used intrinsic potential data rather than direct fish population data, custom grow rooms which may overestimate fish populations , so this difference could be even more extreme. Coho salmon spawn in smaller upstream tributaries that may be particularly susceptible to drought or water withdrawals . This proximity to coho may be explained by the large number of cannabis sites in proximity to small, head water streams , which could further indicate potential threat to other species that depend on these habitats, such as headwaters-dwelling amphibians. Therefore, this proximity to fish habitat could be an ecological concern if farms are drawing water from small rivers or shallow wells during the dry season .

Whether or not metrics summarizing the proximity of farms and sensitive habitats result in actual ecological harm largely depends on the individual land use practices occurring on cannabis farms. There is a rich history of different approaches to cultivating cannabis , which could lead to variation in how cannabis affects ecosystems. Unfortunately, we still do not have a complete picture of cannabis land use practices, nor their mechanisms underlying their ecological effects. So far, available published research suggests that much of small-scale private land cannabis production may not be as ecologically damaging as previously believed , though a consensus has not been reached, and effects may vary over time. Given our current knowledge, therefore, the snapshot of private land cannabis in 2016 in Josephine County does not on its own indicate widespread ecological effects. There could however be an increased concern for local biodiversity if cannabis development expands in size or intensity while remaining in the same spatial configuration—located in forested vegetation, and in proximity to a few key sensitive carnivore and fish species. Certainly, the large number of new farms in the first year of legalization suggest a rapidly expanding industry. This concern suggests a need to consider development pathways and future trajectories that sustain conservation values. The recent boom in outdoor cannabis farming has created a rapid development frontier in the 19 US states that have legalized cannabis production . For decades, outdoor cannabis was grown illegally, often in rural, remote areas, but with state-level legalization, production in those same “legacy” regions has rapidly expanded . In some of these rural, legacy-production regions, cannabis production on private lands can transform development patterns at a regional scale . This development frontier can foster new cultural, economic and demographic dynamics . Importantly, these new patterns of land use also incite concerns for ecological impact related to habitat fragmentation or degradation, potential effects on freshwater quality/availability, and direct or indirect effects on wildlife populations . To understand, reduce, or mitigate these potential impacts, it is important to identify the social and ecological factors that drive cannabis development on private lands across space and time. For example, understanding why farmers choose to cultivate at particular sites may help lawmakers craft and prioritize appropriate regulations for licensed cannabis. Additionally, spatial distribution and socio-cultural drivers are important for understanding where risks of environmental impact or human-wildlife conflict may arise, and for predicting the future trajectory of the cannabis industry. However, there remain many challenges to understanding drivers of cannabis development in these complex systems. Outdoor cannabis production in legacy regions is unique from other forms of traditional agriculture and functions as a closely tied social-ecological system. In these small-scale cannabis systems, the history of illicit farming lays a foundation for production practices that are vastly different from crops that did not have to be concealed, or that were grown following standardized agricultural practices across an industry . Given the continued barriers to bringing legacy farmers into legalized cannabis systems and the existence and persistence of illegal markets, historical context is likely to influence current growing patterns, even as they move into licit markets and expand on private lands . In addition to historical practices that initiated the industry, there are other factors that likely influence whether, where, and how cannabis is produced, including federal, state, and local regulation and enforcement, social acceptance of cannabis within a region, access to education and communication of production practices among growing communities, short- and long-term economic tradeoffs, and others. These factors will influence the spatial distribution and predominant production practices of cannabis over time, which could shift the proximity of cannabis to terrestrial and aquatic wildlife habitats, or alter cannabis impacts on the local environment. These perceived or actual environmental impacts from cannabis can feed back into cannabis land use via shifts in attitudes that could lead to voluntary changes of production practices, increased enforcement, regulatory changes, or shifts in community acceptance for local production .

Some students also indicated that they enjoyed learning about drugs, what is and isn’t a drug, and how to identify harmful substance use behaviors. One student noted the information they learned through RAYS was “valuable” and that they could “take [it] with [them] in life” citing the program as a “second chance” to change their health behaviors. Multiple students also noted the core components of peer involvement and tailored support. One student specifically mentioned how it “wasn’t just adults involved” but also other “kids going through things just like [them], with the same experiences that [they] have”. Enrollees also cited the people involved as important contributors to making the program helpful and enjoyable. Students described the individuals involved in administering and implementing RAYS as welcoming and open-minded, fostering a non-judgmental environment. One student noted how RAYS advocates and staff are “open and listen to what you have to say” and provide valuable support in helping the students obtain the resources they may need.The self-reflective nature of RAYS components was also brought up by multiple students. They noted how RAYS helped them to reflect on the mistakes that they had made in an educational rather than a punitive environment. These alternative to punitive approaches were highly regarded by students as something valuable and engaging. One enrollee said that they “liked that it gave [them] an opportunity to understand what [they] did wrong without just being taken out of school” while also giving them “the chance to take constructive criticism about [their] substance use”. Students also noted the community engagement piece in positive regards as something that allowed them to maintain a relationship with their peers and school community. When asked what they disliked about the program, most students noted that there wasn’t anything they felt that they strongly disliked or would like to see done differently.

Nonetheless, a few enrollees cited aspects related to the logistics of program delivery and knowledge of peer advocates. Some students believed that the program was time-consuming and interfered with their academics. For instance, indoor grow rack two students noted that they were pulled out of class to attend RAYS-related activities as required by their Restorative Plans. A few students also said that the program felt more prolonged compared to just “being suspended and then going back”. Multiple students also felt that much of the program was not a choice, despite RAYS being a voluntary program. One student noted that they felt like they were “forced” to “give a formal apology” while others did not appreciate that some of their peers acted like they did not want to be there. However, a few students did feel that more education and training for the peer advocates was needed. For instance, one student noted that they believed peer advocates needed more training on how to approach sensitive topics that may arise during discussions between advocates and enrollees. When it came to the program timeline, students who cited these aspects had mixed responses. Some believed that more time was needed to be able to complete the required components of their Restorative Plans, while others felt that the program was overextended and time-consuming. Total suspension counts by academic year for RAYS schools and non-RAYS schools are reported in Figure 3. “RAYS schools” represent the four target school sites in Nevada County implementing the RAYS program while “non-RAYS” schools are the selected comparable sites not implementing RAYS. Figure 3 presents the total number of suspensions reported to the CDE for the 2017-18 through 2021-22 academic years at these sites. Prior to the launch of RAYS in August of 2021, Nevada schools reported a higher number of suspensions overall when compared to the non-RAYS sites. Examination of these counts from pre- to post-launch of RAYS reveals a notable difference in the number of suspensions between RAYS and non-RAYS schools, with RAYS schools reporting less than half of those at non-RAYS sites after this time point.

The total number of drug-related suspensions at both RAYS and non-RAYS sites from the 2017-18 to 2021-22 academic year are presented in Figure 4. The CDE considers possession, use, sale, or furnishing of any opiates, opium derivatives, hallucinogenic substances , depressants, stimulants, alcohol, tobacco products and any other controlled substance listed in chapter 2 of the California Health and Safety Code as a drug-related incident warranting suspension. 11,14 In the 2018-19 academic year, Nevada County sites reported almost three times the number of drug-related suspensions in relation to comparable schools . Examination of these suspension counts revealed that after RAYS was implemented in August of 2021 , RAYS schools reported approximately half the amount of drug-related suspensions as the non-RAYS schools.Table 10 provides a breakdown of the exit reasons and completion statuses for all students who exited RAYS during this period . Roughly 4 out of 5 students who exited the program were marked as ‘successful completions’, meaning they completed most if not all the components outlined in their Restorative Plans. Of these successful completions, 17.94% returned and joined RAYS as peer advocates. For the remainder of the sample, RAYS services were found to be inappropriate or unsuitable for 6.25% of students, 4.17% left school or were lost to follow-up, and 8.33% left for another reason. Out of all students, 70.83% completed all components outlined in their Restorative Plans while 29.17% did not. These completion rates are in line with the program objective to reach a 75% successful completion rate among RAYS enrollees by April 2024 .The increasing utilization of restorative practices to address disciplinary incidents in school settings has highlighted the need for evaluations to assess the effectiveness of such programs in lieu of traditional punitive measures. This study reported findings from an intermediate evaluation of an RJP program with AOD use education and treatment counseling components. To properly execute this evaluation, student pretest and posttest survey data was analyzed to assess individual-level impacts of the RAYS program on AOD use behaviors and perceptions of substances, self-responsibility, and resource awareness. Process measures including all activity-level and enrollee case management data were tabulated and examined to assess implementation fidelity in meeting program goals and objectives outlined in the original grant proposal.

Discipline data was used to inform evaluation questions pertaining to changes in overall and drug-related suspensions from pre- to post-implementation of RAYS in Nevada County sites. Of the sample of students who submitted pretest and posttest data , most reported favorable behavioral changes from pre- to post-exposure to RAYS. The majority of RAYS enrollees reported a decrease in past 30-day use of alcohol, marijuana, and vapes with nicotine or just flavoring, indicating a potentially favorable impact of the program in addressing student AOD use behaviors. Additionally, exposure to the RAYS program also seemed to have an impact on student awareness of substance use support and services at their sites, with the majority of students saying they would be able to identify such entities at their schools. In general, students also had positive experiences with RAYS and believed the resources provided were helpful. Examination of the trends in disciplinary incidents from the 2017-18 to 2021-22 academic year revealed a decrease in the total number of suspensions within RAYS schools over time. In relation to comparable sites with regional and demographic similarities, schools at which RAYS was being implemented reported a decreasing trend in suspensions while non-RAYS schools reported an increase. Results from this intermediate evaluation of RAYS also allow for an assessment of implementation fidelity in meeting the original goals and objectives outlined in the project proposal and local evaluation plan . The three main goals of the program were to reduce overall suspension rates at the four target sites, reduce youth marijuana and other substance use, and increase access to SUD treatment services. Relevant process and outcome measures used to inform implementation fidelity assessment and to measure goal and objective attainment are outlined in Tables B and C in the Appendix. Process evaluation measures encompass acquisition of services, RAYS completion rates and statuses , and recidivism rate calculations. The outcome evaluation measures include variables such as the suspension rates, suspension counts, AOD use rates, indoor farming equipment and perceptions of AOD use. Assessment of success in meeting some of these goals and objectives is further discussed in the sections below as they pertain to the relevant process and outcome measure variables. One of the objectives was to reduce marijuana and other substance use among youth who participated in the RAYS program. Based on findings from pretest and posttest data, the majority of students who exited RAYS during the evaluation period reported a decrease in past 30-day use across all products including marijuana, vapes with nicotine or just flavoring, and alcohol. Students who indicated that they used marijuana or vapes reported the highest percent decreases from pretest to posttest. Given that one of the main reasons for establishing RAYS in NCSOS was to address high marijuana, vape, and other drug use rates among students, the decreases seen from pre- to post-exposure of the RAYS program is indicative of the potentially positive impacts on student substance use behaviors. Program components addressing substance use such as the harm reduction classes and SUD counseling may be playing an important role in educating students on the harms of using and providing necessary emotional and mental health support, respectively. However, pretest and posttest data for the harm reduction classes, although outlined in the project proposal, has not been collected due to logistical challenges with survey administration.

Therefore, it is recommended that harm reduction pretests and posttests be administered to students participating in this component in order to assess the specific impacts of these educational sessions on AOD use behaviors and perceptions. Nonetheless, when it comes to harm perceptions, the proportion of students who believed marijuana use was harmful was relatively low compared to other substances. These low harm perception rates for marijuana use reported among the RAYS sample are similar to national trends. For instance, in the most recent report of the MTF survey, about 22% of adolescents in the US perceived regular use of marijuana as risky.2 In comparison, 33.3% of RAYS students thought it was very or extremely harmful to use marijuana every day at pretest. This proportion decreased to 28.6% at posttest, following similar percent decreases reported in recent MTF survey cycles. Nonetheless, perceived harm of everyday vape and alcohol use was higher, but no favorable changes were noted for either product. Low perceived harm may be attributed to a variety of social and environmental factors. For marijuana specifically, researchers have highlighted increased legalization of marijuana for medicinal and recreational purposes as a factor for low harm perceptions among youth. Furthermore, growing public acceptance of marijuana has also been noted as a reason for the decrease in perceived harm among adolescents. This absence in perceived harmfulness of marijuana, despite notable decreases in use rates among RAYS students, sheds light on the potentially significant impacts that social environment and awareness of substances have on adolescent AOD perceptions. Although RAYS may play an important role in addressing use behaviors, these intermediate findings reveal the effects of the program on AOD harm perceptions was minimal. Nonetheless, more research is needed to specifically assess whether harm reduction class components may play a role in this and if not, how course components may be adapted to further target AOD use harm perceptions and awareness. It is crucial to continue monitoring AOD use rates and perceived harm over time in order to inform public health messaging and education efforts. Within the context of RAYS, it is also important that the formal evaluation consider assessing these same AOD use and perception variables at the school-wide level to compare data from the RAYS sample to the broader student body in Nevada County. This comparison will allow for a higher-level examination in order to detect any shifts in school-wide culture with regards to AOD use and harm perceptions. Previous literature has emphasized the importance of gathering data on student experiences in restorative justice programs in order to inform adaptations and enhancements.28 Furthermore, authors also highlight a gap in current restorative program evaluation research with regards to limited or the lack of student feedback in the form of quantitative and qualitative data.

After release, bags were checked for feces, which were removed with ethanol-flamed tweezers and placed in ready glass vials or 2 ml Eppendorf tubes filled with ethanol. They were refrigerated until transport to the United States and then stored at −20°C. Bags were always immersed in a 10% bleach solution, sun-dried, and washed after use. We conducted feeding trials with three common, small insectivores that frequent coffee plantations and were expected to consume the borer: Rufous-capped Warbler , Rufousbreasted Wren , and Plain Wren . Individuals were fed 0, 2, 4, or 8 borers collected in nearby plantations . Specifically, we held each bird’s mouth open and placed the borers inside with a sterilized tweezers. We then used a syringe to inject water into the bird’s mouth, inducing it to swallow the insects. Birds were then placed in mesh cages over sterilized cotton floors. Cages were checked for fecal samples every 15 minutes for 1.5 hours; stressed birds were released earlier. Though referenced in Karp et al. , feeding trial data were not previously analyzed.We poured off ethanol and weighed samples prior to DNA extraction. For all species that were strictly frugivorous, we combined samples derived from different individuals of the same species captured at the same plantation to reduce processing time and cost. The combined sample was homogenized and a <0.25 g subset was extracted. Because many more individuals were captured, black flower bucket in the second year we additionally combined samples from multiple individuals of the same species at the same plantation for all omnivores and large insectivores. Again, samples were homogenized and a <0.25 g subset was extracted. Samples from feeding trials and from small insectivores were always extracted individually. Extraction was performed with kits , modified to increase product yield .

All extractions were accompanied with negative controls with no fecal material added so that we could identify any possible sources of contamination. Following extraction, we ampliftied borer DNA through PCR with borer-specific primers . Each PCR reaction was accompanied with negative and positive controls, derived from feeding trial samples. Though primers were designed to be borer specific, we sent all amplicons of the expected size range for sequencing because many PCR cycles can result in ampliftication of non-target DNA. We used Sequencher to form consensus alignments of DNA reads from forward and reverse primers that were then compared to a borer reference sequence. Only sequences with clean, discernable peaks at target base pairs were analyzed. Those sequences with >98% similarity to borer reference sequences were deemed successful borer identifications. The next most similar sequence from another species in Genbank at the target amplicon was 85% similar.We accidentally contaminated several samples with PCR amplicons, necessitating the development of alternate primers. We ampliftied an 113 bp segment of COI, outside the previous ampliftication region, with forward and reverse primers. Reactions were carried out using the same reagents and protocols, apart from the annealing step . Products were visualized on gels, and negative controls confirmed that the contamination was previous PCR product. Because primers were not borer-specific, all products of the expected size range were sequenced and compared to reference sequences. After the borer, the next most similar sequence in Genbank was 86% similar.We assessed whether confirmed borer predators shared functional traits through compiling a trait database for birds in our study area, focusing on resource and acquisition traits that may affect pest-control provision . We used measurements from birds we captured, and a bird population dynamics dataset collected at 18 nearby sites . Wing chord length and mass were obtained from the population dynamics dataset. We also calculated the total number of captures for each species.

We collected bill width , bill length , and tarsus length from species that we trapped during fecal sample collection. Body lengths were obtained from literature . We gathered behavioral traits from literature . We translated foraging stratum into an ordinal scale , and calculated the average foraging stratum for each species. We quantified diet breadth as the number of food categories consumed . From literature and conversations with local ornithologists , we also identified species that consumed insects and the subset that specialized strictly on insects.We used Generalized Linear Mixed Models to identify variables that significantly influenced the probability of borer DNA detection in feeding trials . The model contained a logit link and binomial error structure, and the feeding trial as a random effect. Species identity, elapsed time since feeding, number of borers fed, fecal sample mass, and 2-way interactions were included as predictors. We then used backwards model selection, iteratively dropping predictor variables and comparing nested models with Aikaike Information Criteria and likelihood-ratio tests . Next, we determined whether species confirmed as borer predators shared traits. Because very few of the birds that were not involved feeding trials tested positive for borer DNA, it was impossible to use logistic regression to associate bird traits with borer predation. Instead, we created a randomization procedure in which six species were drawn at random 1000 times from a species pool , and the average trait value for these species was calculated each time. This procedure generated a null distribution for each trait that could then be compared to the average trait value of confirmed borer predators. If the observed trait value fell outside the 95% confidence interval, then we determined the trait was a significant predictor of borer-predator identity.We ampliftied and sequenced borer DNA from the feces of six species: Buff-throated Foliage-Gleaner , Common Tody-Flycatcher , Rufous-breasted Wren , Rufous-capped Warbler , White-tailed Emerald , and Yellow Warbler . The majority of detections were derived from surveys conducted in 2010 and reported in Karp et al. , even though fewer samples were collected in 2010 . In total, 30 and 27 samples yielded PCR products of the expected size range, 4 and 6 of which were >98% sequence similar to borers.

Though detections rates were low, feeding trials confirmed the efficacy of our approach. Fifteen samples yielded PCR products of the expected size range, all of which were confirmed as borers through sequencing . Additionally, detection probability increased with elapsed time since feeding. For example, models predicted that, for birds that were fed 4 borers and defecated a 0.05 g fecal pellet, detection probability increased from 10% at 20 minutes after feeding to 50% at 80 minutes after feeding. Species identity of feeding trial birds did not influence detection probability. While it is possible that positive borer detections in feeding trials could have resulted from prior consumption, no detections occurred when birds were not fed borers. Moreover, the low detection rates in non-feeding trial birds further reduce the likelihood that positive detections were the result of prior foraging. We found that functional traits differed between confirmed borer predators and other sampled species . Borer predators had narrower bills and shorter wing chords than expected. Though not significant, predators also tended to be smaller, both in mass and length . Diets were specialized , and insectivores were overly represented — only the White-tailed Emerald was not a specialized insectivore. Borer predators were not species of general conservation concern. Predators were equally abundant to other species in our study system, and were neither endemic nor listed on the IUCN red list. Leveraging traits that were over-represented in confirmed borer predators, we predicted other species that may consume the borer but no pest DNA was found in their fecal samples, likely the result of low detection rates .Ecosystem-service management necessitates identifying service providers, especially in the many agricultural systems that are rapidly expanding and intensifying . Our analysis of ~1500 fecal samples documented that six Costa Rican bird species consume coffee’s most damaging insect pest. Still, detection rates were very low: only 0.7% of analyzed samples contained borer DNA. We offer several explanations for low detection. First, we sampled the entire bird community, including frugivores which do not likely consume the borer. Second, borer abundance is low in our study system. Only 2.5% of berries across plantations are currently infested with borers, square black flower bucket whereas infestation has soared above 90% in other countries . Third, detection windows may be narrow. We detected borer DNA in only one sample defecated within 30 min of feeding. Insect DNA could be detected in Carrion Crow feces 30 minutes to 4 hours after consumption . Borers disperse most often and hence are most vulnerable to predation in the afternoon .

Because tropical weather constraints precluded afternoon sampling, a mismatch in sampling and consumption could have depressed detections. Finally, feeding trials demonstrated that false negatives are regular. Models predicted that a positive detection was ~20 times more likely when birds were fed 8 borers and defecated 0.1 g versus 2 borers and 0.01 g. In addition to DNA degradation in the gut, our extraction and PCR procedures may be prone to false negatives. First, PCR inhibitors can persist through extraction and impede DNA ampliftication from fecal pellets . Second, unlike the primers developed by Jaramillo et al. , the primers that we developed were not specific to the berry borer, meaning the primers could have ampliftied DNA from any one of the many species of insects that a bird had recently consumed. Moreover, iterant non-specific PCR binding of either primer set could generate chimeric sequences of multiple species. Accordingly, only 10 of the 57 samples that yielded PCR products of the expected size range were identified as borer DNA after sequencing. Future work could utilize a post-PCR sorting method such as next generation sequencing or cloning to help reduce the frequency of false negatives . Low detection rates suggest that there are other species that consume the borer that we did not identify. The species we did identify, however, shared traits that may be characteristic of these other predators. All identified borer predators except the nectarivorous White-tailed Emerald were strict insectivores. Unsurprising given the borer’s size , borer predators had narrow bills. Additionally, these species had short wings, ideal for navigating the dense coffee understory . It is possible that functional traits would change with a larger sample of predators; however, confirmed borer predators in Jamaican coffee plantations shared many of these traits , supporting our hypothesis that they may help predict other predators . A key difference between our studies, however, is that only one of the species that we identified as a borer predator is migratory . Wecollected our fecal samples during the period of maximum borer dispersal , a time when most migratory species are absent from Costa Rica. Because migratory species could consume borers during their secondary dispersals that occur throughout the year, future work should temporally expand sampling effort to ensure that migratory species are well represented. Our work yielded the critical management insight that managing the predators of crop pests may require looking beyond traditional conservation targets. The six documented borer predators were not rare, endemic, or listed on the IUCN red list. Traditional conservation efforts for threatened species often center on delineating large protected areas. Focusing conservation explicitly in agricultural landscapes could benefit species involved in providing critical ecosystem services to farmers . By confirming that birds consume pests, our work could thus help change attitudes towards biodiversity in human-dominated landscapes by fostering greater recognition of its role in supporting human well being. Species interactions play a pivotal role in many ecologically and economically important ecosystem processes. Uncovering the basic relationships between animals and their food is critical for managing pest control, pollination, seed dispersal, and sanitation . Molecular methods can provide us with a window into these interactions, in some instances for the very first time. Our results demonstrate how identifying just a few key interactions between predators and their prey can yield potential insights for management. Indeed, managing nature to enhance both biodiversity and human well being requires diverse approaches. Techniques and practices have already been borrowed from fields as diverse as agronomy, economics, hydrology, psychology, and sociology. Our results indicate that molecular biology offers ecologists the ability to expand their toolkit in key dimensions and, in turn, advance ecosystem service management.

Some students believed that the program was time-consuming and interfered with their academics. For instance, two students noted that they were pulled out of class to attend RAYS-related activities as required by their Restorative Plans. A few students also said that the program felt more prolonged compared to just “being suspended and then going back”. Multiple students also felt that much of the program was not a choice, despite RAYS being a voluntary program. One student noted that they felt like they were “forced” to “give a formal apology” while others did not appreciate that some of their peers acted like they did not want to be there. However, a few students did feel that more education and training for the peer advocates was needed. For instance, one student noted that they believed peer advocates needed more training on how to approach sensitive topics that may arise during discussions between advocates and enrollees. When it came to the program timeline, students who cited these aspects had mixed responses. Some believed that more time was needed to be able to complete the required components of their Restorative Plans, while others felt that the program was overextended and time-consuming.Total suspension counts by academic year for RAYS schools and non-RAYS schools are reported in Figure 3. “RAYS schools” represent the four target school sites in Nevada County implementing the RAYS program while “non-RAYS” schools are the selected comparable sites not implementing RAYS. Figure 3 presents the total number of suspensions reported to the CDE for the 2017-18 through 2021-22 academic years at these sites. Prior to the launch of RAYS in August of 2021, Nevada schools reported a higher number of suspensions overall when compared to the non-RAYS sites.

Examination of these counts from pre- to post-launch of RAYS reveals a notable difference in the number of suspensions between RAYS and non-RAYS schools, equipment for growing weed with RAYS schools reporting less than half of those at non-RAYS sites after this time point.The total number of drug-related suspensions at both RAYS and non-RAYS sites from the 2017-18 to 2021-22 academic year are presented in Figure 4. The CDE considers possession, use, sale, or furnishing of any opiates, opium derivatives, hallucinogenic substances , depressants, stimulants, alcohol, tobacco products and any other controlled substance listed in chapter 2 of the California Health and Safety Code as a drug-related incident warranting suspension. 11,14 In the 2018-19 academic year, Nevada County sites reported almost three times the number of drug-related suspensions in relation to comparable schools . Examination of these suspension counts revealed that after RAYS was implemented in August of 2021 , RAYS schools reported approximately half the amount of drug-related suspensions as the non-RAYS schools.Table 10 provides a breakdown of the exit reasons and completion statuses for all students who exited RAYS during this period . Roughly 4 out of 5 students who exited the program were marked as ‘successful completions’, meaning they completed most if not all the components outlined in their Restorative Plans. Of these successful completions, 17.94% returned and joined RAYS as peer advocates. For the remainder of the sample, RAYS services were found to be inappropriate or unsuitable for 6.25% of students, 4.17% left school or were lost to follow-up, and 8.33% left for another reason. Out of all students, 70.83% completed all components outlined in their Restorative Plans while 29.17% did not. These completion rates are in line with the program objective to reach a 75% successful completion rate among RAYS enrollees by April 2024 .

The increasing utilization of restorative practices to address disciplinary incidents in school settings has highlighted the need for evaluations to assess the effectiveness of such programs in lieu of traditional punitive measures. This study reported findings from an intermediate evaluation of an RJP program with AOD use education and treatment counseling components. To properly execute this evaluation, student pretest and posttest survey data was analyzed to assess individual-level impacts of the RAYS program on AOD use behaviors and perceptions of substances, self-responsibility, and resource awareness. Process measures including all activity-level and enrollee case management data were tabulated and examined to assess implementation fidelity in meeting program goals and objectives outlined in the original grant proposal. Discipline data was used to inform evaluation questions pertaining to changes in overall and drug-related suspensions from pre- to post-implementation of RAYS in Nevada County sites. Of the sample of students who submitted pretest and posttest data , most reported favorable behavioral changes from pre- to post-exposure to RAYS. The majority of RAYS enrollees reported a decrease in past 30-day use of alcohol, marijuana, and vapes with nicotine or just flavoring, indicating a potentially favorable impact of the program in addressing student AOD use behaviors. Additionally, exposure to the RAYS program also seemed to have an impact on student awareness of substance use support and services at their sites, with the majority of students saying they would be able to identify such entities at their schools. In general, students also had positive experiences with RAYS and believed the resources provided were helpful. Examination of the trends in disciplinary incidents from the 2017-18 to 2021-22 academic year revealed a decrease in the total number of suspensions within RAYS schools over time. In relation to comparable sites with regional and demographic similarities, schools at which RAYS was being implemented reported a decreasing trend in suspensions while non-RAYS schools reported an increase.

Results from this intermediate evaluation of RAYS also allow for an assessment of implementation fidelity in meeting the original goals and objectives outlined in the project proposal and local evaluation plan . The three main goals of the program were to reduce overall suspension rates at the four target sites, reduce youth marijuana and other substance use, and increase access to SUD treatment services. Relevant process and outcome measures used to inform implementation fidelity assessment and to measure goal and objective attainment are outlined in Tables B and C in the Appendix. Process evaluation measures encompass acquisition of services, RAYS completion rates and statuses , and recidivism rate calculations. The outcome evaluation measures include variables such as the suspension rates, suspension counts, AOD use rates, and perceptions of AOD use. Assessment of success in meeting some of these goals and objectives is further discussed in the sections below as they pertain to the relevant process and outcome measure variables.One of the objectives was to reduce marijuana and other substance use among youth who participated in the RAYS program. Based on findings from pretest and posttest data, the majority of students who exited RAYS during the evaluation period reported a decrease in past 30-day use across all products including marijuana, vapes with nicotine or just flavoring, and alcohol. Students who indicated that they used marijuana or vapes reported the highest percent decreases from pretest to posttest. Given that one of the main reasons for establishing RAYS in NCSOS was to address high marijuana, vape, and other drug use rates among students, the decreases seen from pre- to post-exposure of the RAYS program is indicative of the potentially positive impacts on student substance use behaviors. Program components addressing substance use such as the harm reduction classes and SUD counseling may be playing an important role in educating students on the harms of using and providing necessary emotional and mental health support, respectively. However, pretest and posttest data for the harm reduction classes, although outlined in the project proposal, has not been collected due to logistical challenges with survey administration. Therefore, it is recommended that harm reduction pretests and posttests be administered to students participating in this component in order to assess the specific impacts of these educational sessions on AOD use behaviors and perceptions. Nonetheless, when it comes to harm perceptions, the proportion of students who believed marijuana use was harmful was relatively low compared to other substances. These low harm perception rates for marijuana use reported among the RAYS sample are similar to national trends. For instance, in the most recent report of the MTF survey, grow tables 4×8 about 22% of adolescents in the US perceived regular use of marijuana as risky. In comparison, 33.3% of RAYS students thought it was very or extremely harmful to use marijuana every day at pretest. This proportion decreased to 28.6% at posttest, following similar percent decreases reported in recent MTF survey cycles. Nonetheless, perceived harm of everyday vape and alcohol use was higher, but no favorable changes were noted for either product. Low perceived harm may be attributed to a variety of social and environmental factors. For marijuana specifically, researchers have highlighted increased legalization of marijuana for medicinal and recreational purposes as a factor for low harm perceptions among youth. Furthermore, growing public acceptance of marijuana has also been noted as a reason for the decrease in perceived harm among adolescents. This absence in perceived harmfulness of marijuana, despite notable decreases in use rates among RAYS students, sheds light on the potentially significant impacts that social environment and awareness of substances have on adolescent AOD perceptions.

Although RAYS may play an important role in addressing use behaviors, these intermediate findings reveal the effects of the program on AOD harm perceptions was minimal. Nonetheless, more research is needed to specifically assess whether harm reduction class components may play a role in this and if not, how course components may be adapted to further target AOD use harm perceptions and awareness. It is crucial to continue monitoring AOD use rates and perceived harm over time in order to inform public health messaging and education efforts. Within the context of RAYS, it is also important that the formal evaluation consider assessing these same AOD use and perception variables at the school-wide level to compare data from the RAYS sample to the broader student body in Nevada County. This comparison will allow for a higher-level examination in order to detect any shifts in school-wide culture with regards to AOD use and harm perceptions. Previous literature has emphasized the importance of gathering data on student experiences in restorative justice programs in order to inform adaptations and enhancements.28 Furthermore, authors also highlight a gap in current restorative program evaluation research with regards to limited or the lack of student feedback in the form of quantitative and qualitative data. This study sought to address this gap in data collection through the inclusion of open text and quantitative items in the posttest instrument to gather data on student perceptions of the RAYS program and their overall experiences. Items included in the survey assessed resource awareness, self-reflection of AOD use, and the likelihood of recommending the RAYS program to peers. Overall, student resource awareness increased for substance use treatment and support services. More students believed they could find a resource or individual for themselves or a peer after going through RAYS. This puts RAYS on track to meet the program objective of increasing access to and awareness of SUD treatment services . Another quantifiable objective was to provide SUD treatment services to at least 100 students by April 2024. Based on case data, of the 63 students who enrolled in RAYS during this evaluation period, 40 were referred and participated in at least one substance use counseling session . Therefore, at this state, the program is just under 50% of the target goal. It is important to note that not all students who go through RAYS are diverted from drug-related disciplinary incidents, thus may not require SUD treatment services.Prior to the launch of the RAYS program, Nevada County sites reported higher overall and drug-related suspensions in relation to selected comparable sites in a neighboring county. Overall suspension counts were notably higher in Nevada County schools, reporting over double the number of suspensions in relation to comparable sites during the 2017-18 and 2018-19 school years. Nonetheless, a sharp decline in the 2021-22 academic year, after the launch of RAYS, implies some effect of the program on the disciplinary landscape in Nevada County sites. This notable reduction in the number disciplinary incidents after implementation of an RJP program has been reported in previous studies. Hashim and colleagues noted that at the 1-year mark after implementation of a restorative justice program, there was a significant reduction in the number of suspensions. This same trend is seen in the initial decline in the number of disciplinary incidents in Nevada County sites implementing RAYS.

Operators should take a closer look at likely survivability given current field experience from other similar arrays. For low sloped roof and ground array types, operators can utilize guidance produced by the Structural Engineers Society of California to examine the integrity of existing structures. Though intended to be used for new systems, operators can use the guidance to identify weaknesses and then engineer a retrofit. System operators have been confronted with solar arrays vulnerable to even minor and routine wind forces, indicating a design or installation flaw more than a severe weather vulnerability. Post-storm inspections of solar arrays have found significant damage from routine thunderstorms that had wind speeds of 70 mph or less. In one example, lab inspectors found a ground array in Prescott, Arizona completely destroyed due to fastener and racking weaknesses. A weather station located within feet of the array recorded 70 mph winds from a summer monsoon storm that caused the damage. Cannabis sativa L. belongs to the Cannabaceae family that contains the genera Cannabis and Humulus , as well as eight genera that were previously classified as Celtidaceae. 1 In the formal botanical nomenclature of C. sativa, this single species of the Cannabis genus contains two subspecies, each with two varieties. These include C. sativa subsp. sativa var. sativa, C. sativa subsp. sativa var. spontanea, C. sativa subsp. indica var. indica, and C. sativa subsp. indica var. kafiristanica. Aside from the botanical classification, drying room it has been proposed that, instead of the commonly used designations of “cultivars” and “strains”, C. sativa should be categorized as chemovars according to the chemical profiles of phytocannabinoids and terpenes in flowers. 

Among the chemicals produced in C. sativa, two phytocannabinoids, the psychoactive compound Δ9 -tetrahydocannabinol and the medicinally important, but nonpsychoactive, compound cannabidiol , have been intensively studied for their structures, biosynthesis, and biological activities. Additional phytocannabinoids, and other classes of plant chemicals, such as terpenes, flavonoids, and alkaloids, have also been identified in C. sativa. These other plant chemicals exert synergistic effects to enhance the bio-activities of phytocannabinoids, known as “the entourage effect”. However, the underlying mechanisms of the entourage effect are not well understood. As such, studies on non-phytocannabinoid compounds, such as terpenes and flavonoids, are valuable for developing therapeutics in C. sativa. More than 20 flavonoids have been identified in C. sativa, most of which are flavone and flavonol aglycones and glycosides. Interestingly, three prenylated/geranylated flavones, cannflavin A, B, and C, were isolated in C. sativa . It is worth noting that, although cannflavins are often referred to as flavonoids unique to C. sativa, cannflavin A has also been identified in Mimulus bigelovii, a plant in the Phrymaceae family. Since biosynthesis of the core flavonoid skeleton in plants and bio-activities of the common flavones and flavonols have been widely studied and reported, this mini-review will focus on the biosynthesis and bio-activities of the relatively unique cannflavins as well as the applications of C. sativa flavonoids.The phenylpropanoid and flavonoid biosynthetic pathways build the core skeletons of flavonoids in C. sativa . Genes encoding two enzymes in the phenylpropanoid biosynthetic pathway, phenylalanine ammonia-lyase and p-coumaroyl: CoA ligase , were isolated in C. sativa var. Futura by searching expressed sequence tags using homologous PAL and 4CL sequences from other plants. 

Conversion of p-coumaroyl CoA to luteolin encompasses condensation with three molecules of malonyl CoA to form naringenin chalcone by chalcone synthase ,ring closure of naringenin chalcone to generate naringenin by chalcone isomerase , formation of apigenin from naringenin by flavone synthase , and 3-hydroxylation of apigenin to derive luteolin by flavonoid 3′-hydroxylase . Based on their chemical structures, cannflavin A and B could be derived from luteolin through transferring a methyl group to the 3′-O position by a methyltransferase activity as well as a geranyl group or a prenyl/ dimethylallyl group to the C6-position by a prenyltransferase activity . Candidate methyltransferases and prenyltransferases responsible for these reactions were identified from a draft C. sativa genome assembly based on sequence homology to previously characterized enzymes and phylogenetic analysis. Upon functional characterization using purified recombinant proteins, it was shown that a regiospecific O-methyltransferase methylates the 3′-O position of luteolin and forms chrysoeriol, and a prenyltransferase adds a geranyl or a prenyl group to chrysoeriol and produces cannflavin A and B. However, the function of CsOMT21 and CsPT3 in cannflavin biosynthesis has not been demonstrated in a plant system. To date, flavonoid identification in C. sativa has focused on plants that are grown under non-stressed conditions. While flavonoids are present in most tissues studied in C. sativa, including seedlings, leaves, flowers, and fruits, they are undetectable in roots and seeds. In addition to the tissue specific distribution, flavonoid profiles were also shown to vary in bracts during plant development. As many flavonoids possess protective functions for plants, their production is responsive to environmental factors, which is also observed in C. sativa. For example, cannflavin A accumulation is determined not only by the genetic background, but as a response to temperature, solar radiation, rainfall, and humidity in the environment. 

Moreover, higher elevation positively impacts the content of cannflavin A, B, and C in cloned C. sativa plants grown at different altitudes. With these observations taken into consideration, it is tempting to postulate that, aside from the flavonoids that have already been isolated in C. sativa tissues, some yet unidentified flavonoids may only be produced under specific environmental conditions, such as biotic and abiotic stresses. It is also possible that certain flavonoids only accumulate in significant quantities in specific C. sativa chemovars, such as cannflavin C that was isolated and identified from a high THC chemovar. As such, unraveling the identity of additional flavonoids, particularly those unique to C. sativa, will facilitate a comprehensive understanding of the biosynthesis and functions of flavonoids in this important plant.Besides the antioxidative effects that cannflavins share with many other flavonoids, a relatively well-studied bio-activity for cannflavins is their anti-inflammatory properties. An intriguing observation was first reported in 1981, showing that compounds present in a phytocannabinoid-free extract of C. sativa leaves could be involved in the production or release of prostaglandin E2 in mice. Further work showed that cannflavins in ethanolic extracts of C. sativa leaves inhibited 12-O-tetradecanoylphorbol-13-acetate – induced PGE2 production in cultured human rheumatoid synovial cells. Chemical structures of cannflavin A and B were subsequently solved using nuclear magnetic resonance and demonstrated to be prenylated/geranylated flavones. More recently, it was shown in in vitro enzyme assays that cannflavin A and B exert anti-inflammatory activities by inhibiting the microsomal PGE2 synthase-1 and the 5- lipoxygenase activities, leading to reduced PGE2 and leukotriene production, respectively. Cannflavin A and B show promise as an anti-inflammatory therapeutic agent because they were about 30 times more effective than aspirin in inhibiting PGE2 release when assayed in human rheumatoid cells. An additional advantage is that cannflavin A only weakly inhibits cyclooxygenases COX-1 and COX-2, and therefore can circumvent the adverse side effects exhibited by COX inhibitors , such as gastrointestinal erosion. The neuroprotective role of cannflavin A was explored in neuronal PC12 cells. At 10 μM or lower concentrations, cannflavin A enhanced the viability of neuronal PC12 cells against amyloid β -induced cytotoxicity by reducing Aβ1−42 aggregation and fibril formation. Anticancer activities were reported for a synthetic 8-prenylated isomer of cannflavin B, isocannflavin B . Isocann- flavin B suppressed the proliferation of estrogen-dependent T47-D human breast cancer cells through a G0/G1 cell cycle arrest. It also increased apoptosis in two pancreatic cancer cell lines Panc-02 and Ptf1/p48-Cre . Treatment with isocannflavin B caused a delay in both local and metastatic tumor progression and increased survival in mice with pancreatic cancer . These reports suggest the potential of isocannflavin B as an anticancer drug, though translational studies should be undertaken to determine its activities in humans. A combination of in vitro bioassays and in silico molecular docking analysis established antiparasitic activities of cannflavins. Cannflavin A and cannflavin B exhibited moderate anti-leishmanial activities against a culture of Leishmania donovani promastigotes. The bio-assay results were corroborated by strong docking energy of cannflavin A to one of the protein targets in L. donovani, Leishmania pteridine reductase 1. Besides L. donovani, cannflavin A also showed moderate inhibitory activity against the parasite Trypanosoma brucei brucei with an IC50 value of 1.9 μg/mL. The mechanistic basis for the antiparasitic effects of cannflavins remains to be elucidated. To date, vertical farming units only molecular docking/computational analysis has been employed to evaluate the antiviral activities of cannflavins. Cannflavin A showed a relatively high binding affinity and high reactivity against HIV-1 protease, an enzyme that renders human immunodeficiency viruses infectious, as determined by the density functional theory analysis. A molecular docking study of multiple protein targets of Dengue virus revealed cannflavin A as a strong docking ligand for the Dengue virus envelope protein. Cannflavin A is also among the phytochemicals that are predicted to show efficient docking to the helicase , helicase , methyltransferase , and RNA-dependent RNA polymerase of Zika virus. Although the computational analysis suggests cannflavins as potential antiviral drug leads, further empirical evidence is still needed to precisely determine their bio-activities.

To investigate the microbial metabolism of cannflavins, cannflavin A and B were fermented with Mucor ramannianus and Beauveria bassiana , which resulted in 6″S,7″-dihydroxycannflavin A, 6″S,7″-dihydroxycannflavin A 7-sulfate, and 6″S,7″-dihydroxycannflavin A 4′-O- α-L-rhamnopyranoside from cannflavin A, and cannflavin B 7-O- β-D-4′″-O-methylglucopyranoside and cannflavin B 7-sulfate from cannflavin B. However, these microbial transformed metabolites do not possess the antimicrobial and antiparasitic activities reported for cannflavin A and B. Whether and how the geranylation and prenylation at C6 of cannflavin A and B and at C8 of cannflavin C and isocannflavin B contribute to their anti-inflammatory, neuroprotective, anticancer, antiparasitic, and antiviral activities should be further investigated. Insights into the structure− function relationship of these prenylated/geranylated flavones will inform the effective development of therapeutics. Furthermore, microbial degradation products of cannflavins in humans need to be elucidated to better understand drug metabolism and biological functions of cannflavins in humans.Molecular and genetic studies in C. sativa have lagged behind many other plant species due to its historically prohibited status. However, the advancements in omics methods and the availability of genome and transcriptome sequences in the public domain have largely facilitated molecular studies in C. sativa. A draft genome of cultivated C. sativa was released in 2011, although it was not assembled to the chromosomal level. Recently, a high-quality reference genome of a wild C. sativa variety was obtained using PacBio, a single-molecule real-time sequencing technology, and Hi-C, a next-generation sequencing technology for chromosome conformation capture. With the assistance of transcriptome sequencing, 38,828 protein-coding genes were delineated, over 98% of which were functionally annotated. In the past few years, there have also been increasing efforts in sequencing the transcriptomes of multiple chemovars and wild C. sativa. As of January 2021, 59 C. sativa-related bioprojects have been registered in the National Center for Biotechnology Information Sequence Read Archive database, a repository of high-throughput sequencing data. Of these bio-projects, 37 contain genome or transcriptome sequences of plant materials. These bio-projects aim to discover genes responsible for the biosynthesis of C. sativa phytochemicals, to elucidate the evolution and genetic diversity of C. sativa accessions, or to examine changes in transcriptomes when C. sativa plants are exposed to abiotic stresses. These sequencing data collectively are invaluable for gene discovery, biological application, and genetic improvement of C. sativa. Indeed, the utility of C. sativa genome sequences has already been demonstrated in cloning genes encoding the prenyltransferase and methyltransferase enzymes for cannflavin biosynthesis. On the other hand, transcriptome data that are publicly available or generated in individual research groups will be useful for elucidating the flavonoid biosynthetic and regulatory genes using gene coexpression analysis. In addition to transcriptomic analysis, integrated analysis of transcriptome, metabolome, and proteome data can be utilized to reveal genes responsible for the spatial and temporal distribution of flavonoids in C. sativa regulated by plant development and/or the environment. Because it is an integral part of the complex metabolic network, understanding the control of flavonoid production will have implications in the accumulation of phytocannabinoids and other non-phytocannabinoid chemicals in C. sativa. Moreover, understanding the control of stressinduced flavonoids will facilitate the development of environmentally resilient C. sativa plants. The bio-activities of cannflavins and other flavonoids make them a desirable bioproduct that will require the biosynthesis of a large amount of flavonoids for downstream applications. However, flavonoids are present at low levels in C. sativa tissues grown under normal conditions.

Gathering and selling of wild species has intensified since the collapse of the Soviet Union in 1991 . With current high unemployment rates in the region exacerbated by the pandemic, locals continue to rely on gathering and selling wild food species. More than half of local Hutsuls in interviews described the economic and cultural value of gathering plants – an economy of gathering. Species that are culturally, nutritionally, and economically valued can be split into the following categories: mushrooms, lichens, berries, and roots. Many of these species are found on polonynas, alpine meadows and forests. The more remote a village is from roadsides and grocery stores, the more gathering for personal use is practiced. Often these species are also collected for further sale. Not only does gathering provide food and medicine; it is also a cultural activity that upholds personal and community well being through religious ceremonies, harvesting, and processing, while building and maintaining community relationships . These types of food systems not only provide medicinal and nutritional needs, but also present an active opportunity to connect with the land, which in turn allows community members to, quite literally, nourish one another. As mentioned earlier, gathering at different seasons diversifies the timing of impacts on landscape, allowing for regeneration and growth. Additionally, there is an understanding that each year’s harvests will be variable and subject to change based on impacts of externalities In terms of providing supplemental income, the sale of all these species helps subsidize costs to buying other food items, school supplies, clothing, and household cleaning supplies. Forest species are used primarily for filling income gaps, cannabis grow equipment which is a continuous cycle. In a recent study analyzing Hutsul forest use in Northern Bukovina versus Southern Bukovina , Hutsuls in Ukraine expressed more dependence on forests, stating that selling berries and mushrooms was a primary source of income .

In this collaborative study, the economy of gathering, as an adaptive strategy, also underlines Hutsul forest dependence, promotes trade within and beyond communities, and allows for the supplementation of income while also recognizing the variability of local markets based on seasonal cycles of harvest and resource use. While the economy of gathering provides a local flow of income, is important to note an external force in the region – commercial harvesting. Locals noted a rise of commercial berry and medicinal plant harvesting in the Carpathian Mountain region. Arnica montana, a plant prevalent in local markets, is also noted to have suffered a population decline due to the over-harvesting. In addition, there has been a rise of commercial harvesting of endangered plants such as Rhodiola rosea and Gentiana lutea. Rhodiola rosea has been greatly impacted due to industrial production, with tinctures being very popular. However, as noted by elders, Rhodiola rosea roots need 3-4 years to mature and, because of early harvesting, local plant populations have diminished. In addition, international medicinal plant companies have shown a growing interest in harvesting medicinal plants in the Carpathians and target vulnerable plant species. To address the demand for medicinal plants, various national parks have integrated the development of medicinal plant plantations to offset the endangered status of native medicinal plants such as Arnica montana and Rhodiola rosea. As stated by a local park authority, these plants are grown in controlled outdoor environments and, for tinctures to be as effective, proportions need to be amplified by 20- 30% in tinctures to be just as effective as wild plant harvests. External commercial harvesting of culturally relevant plants such as Arnica montana, Rhodiola rosea and Gentiana lutea in Hutsulshchyna, in addition to regional impacts of illegal logging and climate change present layers of complexity in retaining local social resilience. There is a tension between local economies and external economies . As explained by numerous elders, “once gathering becomes a business, there [also] appears a consumer and corporate interest.” Most elders in the region adamantly oppose putting medicinal plants in the rank of industrial production due to accompanying habitat destruction.

Intensive commercial harvesting in the region began 20-30 years ago and has impacted the region and endemic plant populations. There is a local saying, “After me, [there will be] a flood,” reflecting the business-driven aspect of over-harvesting. It implies that environmental destruction is an inevitable result of corporate presence. Both logging and increased mean temperatures increase erosion, causing an uptick of hydrological events such as flooding in the region . In terms of maintaining resilience, the local economy of gathering is reliant on a broad range of species inhabiting a range of environments both temporally and spatially, and invites a constant dialogue between communities and the landscape. Additionally, local gathering is based upon gathering methods that are selective and specific to the species. This knowledge is embedded within the local communities and serves as a participatory method of resource monitoring. Local, place-based economies are resilient by nature, while extractive economies tend to be divorced of the immediate needs, values, and ecocultural memories of locals reliant on those landscapes.Fallback foods are yet another adaptive strategy. Fallback foods consist of mostly plant species that serve as nutritional support during times of restricted movement during war, crop failure, weather , and disease. Many of these species are still culturally important and provide a variety of functions in the nested habitats in the Carpathian Mountains for at least the last century. During the famines of the 19th and 20th centuries, gathering of wild species provided a source of medicine and food for Ukraine . Hutsulshchyna has experienced social and environmental disturbances due to the impacts of war, colonial occupations, and violence. This region has experienced battles with invasions from the Tartar hordes , the Polish regime , and the Austrian-Hungarian Empire . In the interwar period, Hutsulshchyna was divided at the borders with the central part belonging to Poland, the southern and eastern part under Romania, and the western part under Czechoslovakia . The part of Hutsulshchyna in this study was under Poland , then German occupation and then Soviet Union . Political boundaries running through the territory have had less effect on Hutsul unity since it is the mountains that form the natural boundary among states, not the artificial lines drawn through it .

The geography of the Carpathian Mountains served as a buffer up until late 1930s against political terrors, war, genocide, and violence waged in Ukraine by German Nazis, Soviet Communists and Russian czars. The Austrian-Hungarian colonization of Hutsulshchyna meant that this region was spared from the Holodomor of 1932-1933, a Soviet-Russian orchestrated genocide in Central and Eastern Ukraine . However, in interviews, elders mentioned that another Soviet famine of 1946-1947 affecting Ukraine, Moldova, Russia, and Belarus , caused an influx of Moldovans to migrate to the Carpathian Mountains. These demographic shifts caused more reliance on neighboring ecologies and plant usage. Many fallback species documented during famine or war in the early 20 th century are still embedded in use today. While literature highlights a deep history of berry and mushroom reliance during times of scarcity in Ukraine, finding information on fallback foods in Hutsulshchyna is both scattered and primarily written in Polish. Hutsulshchyna, along with Western Ukraine, was under Polish Republic rule from 1918-1939 and books by Ukrainian authors were censored and scholarly ethnographic works were mainly published in Polish. In the postwar years, literature surrounding Hutsulshchyna was written but there is practically no focus on foods. Finally, indoor grow cannabis most Hutsul TEK is passed down generationally and infrequently documented in written form. It is important to note that there is rich knowledge embedded in the daily rhythms of life that cannot be captured in an extensive literature review or interviews. This knowledge has survived and thrived despite colonization, famine, and war. Here, we offer a sliver of ecocultural memory of fallback foods in Hutsulshchyna. Many of the species mentioned as fallback foods by Polish ethnographers in the early 20th century are still used in diverse ways . It is frequently during times of scarcity that species use transitions from a medicine or seasoning to a food. Knowledge of plant use transformation is embedded in ecocultural memory. Interestingly, according to Lukasz’ analysis of Fischer’s work, as early as 1934, memory of wild plants used in times of shortage was fading and respondents spoke about using fallback foods in both past and present tenses . However, in Hutsul counties of 1934, the people talked about fallback foods being used presently in 94% of places. Many of these same plants are still used today. Past uses inform present formation and retention of ecocultural memory, thus propelling and ensuring future sustainability. In this case, there is little distinction between specific fallback foods used only during times of scarcity and those used today. Instead, these critical species are nested within everyday cultural uses of medicine, seasoning and food, thereby ensuring a long-term adaptive strategy. Short-term responses and long-term responses result the cultivation of food sovereignty as seen in traditional foods in the region. In rural Hutsulshchyna, households produce most of their own food, relying on various ecosystems. In Ivano-Frankivsk province, which encompasses the area of the Hutsulshchyna in this study, 42.8% of average monthly monetary expenditure is spent on food and non-alcoholic drinks . In addition to gathering wild species from a range of multi-functional landscapes, livelihood is also composed of community-derived resources including agricultural animals , supplying both dairy and meat. Rivers and ponds provide opportunities for fishing. Beekeeping is a common activity, with the endemic Carpathian bee providing honey. Grazing occurs on pastures, fields, polonynas, tolokas, gardens and forests. Gardens typically contain a variety of trees including sweet cherry, cherry, plum, apricot, apple, pear, nut trees along with perennial bushes including strawberry, raspberry, currant, gooseberry and grape. In the Carpathian Mountains, home gardens provide a source of food and medicine. These microenvironments within the agroecosystem create another function and layer of resilience in a larger ecosystem. They act as centers of experimentation, introduction, and crop improvement. In some cases, elders mention transplanting wild plant species into their own home gardens including Fragaria vesca and medicinal root species such as Rhodiola rosea and Arnica montana. Hutsul dialogue with diverse habitats is maintained seasonally; during times of harvest of wild plants and mushrooms as well as during plowing, sowing, and harvesting of home gardens. It is the maintenance of traditional foods that reaffirm both sustenance and cultural connections to various ecosystems for Hutsuls, forest-dependent communities in the Carpathian region. Culturally important species are used in a variety of traditional foods. Many commonly gathered berry species are traditional foods including Vaccinium species , Rubus species , Ribes species , Fragaria vesca, Sambucus nigra, Aronia melanocarpa, and Sorbus aucuparia. Berries are eaten fresh, frozen, and dried, or cooked into jams, jellies, fillings for traditional dumplings, syrups, and sauces, or used in recreational drinks including fermented kvass, as well as juice, uzvar , and wine. The culturally important bilberry is cooked into varenyky , and used as a flavoring in alcoholic tinctures, fruits, and juice. In terms of health benefits, there are diverse phytochemicals present in berries, specifically wild berries of the Vaccinium genus, which are seasonally harvested. Wild Vaccininum berry species are renowned for their high concentrations of phenolic and polyphenolic compounds that interact to improve human health . In addition to berries providing a source of vitamins and medicine, they also infuse an array of flavor to teas, recreational drinks, jams, and jellies. Raspberries are consumed recreationally, and their leaves, stem, and berries used as a medicinal tea. Wild raspberries have slightly better medicinal properties, taste, and aroma than garden raspberries. Chokeberry has a wide range of uses including consumption as a fruit, tea, kvass, wine and as a medicinal tincture. Hutsul traditional dishes incorporate an important dairy product from polonynas, , a cheese made from Carpathian cows or sheep, and as well as many mushroom species . Mushrooms are used traditionally in cooking and in holiday meals . Most people and families go out and gather mushrooms in summer and fall, a recreational and seasonal intergenerational activity. For example, one elder mentioned, “I take my grandson and we go together to pick mushrooms. I show him the place where mushrooms grow.” [Mykola ] Mushrooms also serve as an important food source, being very popular during winter holidays, where large quantities of marinated mushrooms, and mushroom dishes are made.

The Soviet regime had a particularly brutal impact on tradition, language, land use and life ways. Between 1945-1991 Soviet policy required nationalization, including mass collectivization of farms . Forests were transferred to the state, and private plots of land were joined into collective farms. All land was surrendered and tilled for the benefit of the larger state . During this era, clearing of Carpathian forests for agricultural development, specifically kolhospy, led to habitat fragmentation, leading to of planted spruce monoculture forests . How did these land management policies impact the gathering of plants and mushrooms? Ethnobotanical gathering practices did not stop under any colonial rule. However, these policies impacted the extent of access to land and forests where these species live, thereby shifting relationships to land and environment . Scholars state that Soviet policies also caused hybridization of Soviet knowledge into local ethnobotanical knowledge in Ukraine . After the historic collapse of the Soviet Union, tens of thousands of workers faced unemployment, which catalyzed rural depopulation and migration for work outside of Ukraine . This migration also led to the decline of traditional agricultural systems, which also changed the landscape, causing the reduction of secondary grasslands . In response and despite all these stressors, Hutsul communities’ reflexive response continues to be subsistence farming along with a gathering of wild species, fishing, and game. Today, ecosystem challenges include illegal and destructive logging, the rise of ecotourism and accompanying infrastructure development, commercial harvesting of wild species, and climate change impacts . Introduction of Ukraine to the market economy has resulted in the privatization of state properties leading to the rise of ski resorts in the region. As the main regional challenge,vertical farming supplies illegal logging is managed by organized criminal networks under the guise of semi-legitimate businesses and corporations .

The main avenues of illegality include falsification of paperwork along the supply chain, as well as fraud and collusion with government officials . Minimal legal and financial penalties tend to make these activities fairly appealing within organized crime networks. However, more recently, the use of multi-time satellite images, DNA and isotope analyses of wood, along local citizen activism has helped combat illegal logging in the region. . According to local Hutsul knowledge holders, logging in this region encourages succession of species such as Rubus idaeus, Rubus caesius, Vaccinium myrtillus, Chamaerion angustifolium, Orchis macula, and Aronia melanocarpa. These species are used, appreciated, and gathered fairly frequently but they are also noted to be highly invasive and hinder forest growth. The gathering of these species helps curb their encroachment. Illegal logging also impacts mushroom growth and nutrient cycling, weakening overall forest health. Additionally, Hutsul knowledge holders stress the impacts of external commercial harvesting of culturally important species including Vaccinium myrtillus, Arnica montana, Cetraria islandica, and Gentiana lutea has increased in recent years. Commercial harvesting of important NTFPs raises concerns expressed by communities impacted by these practices worldwide, including: 1) intensified impacts on habitats, 2) increased harvest volumes, 3) restricted access to land, as well as 4) changes in financial and technological incentives promoting intensive harvesting . In this context, commercial harvesting impacts the curative qualities of medicinal plants harvested, reduces accessibility to habitats and availability of these culturally important species to local Hutsul populations. Lastly, the convergence of colonial policies of forest mismanagement and rising threats of climate change have compounded the rise of pine bark beetle invasion. With all these factors impacting culturally important habitats such as woodlands and forests, relationship to land have been continually challenged and threatened by external governance structures. Accessibility to place in Hutsulschyna, a socio-ecological-political issue, is beginning to be addressed through the reconciliation of harmful historic forest management practices and illegal logging practices.

Despite these continual and traumatic eco-cultural-political stressors, the dialogue between landscape and Hutsul communities has not weakened. It through the continual gathering of wild and cultivated species that relationship, community needs, traditional food, and place remain intertwined and inseparable . It is the ecology of the forest understory that provides both culturally important plants and mushrooms providing for multiple needs such as food and medicine nested in cultural practice. Hutsul management of polonynas or alpine meadows, harbors successive sets of plant communities and important root medicines like arnica , and Gentian root species . In general, floral composition of polonynas is incredibly diverse, harboring a high proportion of species and habitats that are almost completely absent in the forest belt below. Ultimately, the diversity of habitats in Hutsulshchyna – garden, roadside, field, pasture, meadow, woodland, forest, toloka, alpine meadow and polonyna – provide a range of landscape interaction as well as a diversity of species use and reliance. By integrating quantitative and qualitative approaches, a more synthesized and community driven understanding of the role of ethnobotany surrounding the cultural historical center of Hutsulshchnya of Verkhovyna arises. As this study shows, an integrated qualitative and quantitative approach is necessary to elucidate the context of ethnobotanical use and communityuse implications surrounding responses to historic and present ecosystem challenges. Quantitative ethnobotanical indices , Cultural Importance , Frequency of Citation , Number of Uses , Relative Frequency of Citation , Relative Importance ) reveal information about 108 species from 79 genera and 48 families including 23 species of cultivated plants, 9 species of mushrooms and 2 species of lichens. Carpathian forests, mountains, woodlands, fields, alpine meadows, pastures, meadows, polonynas, tolokas, and roadsides serve as a biocultural reservoir for wild plant, mushroom, and lichen species while home gardens serve as places of experimentation of domesticating wild species and diversification of therapeutic remedies using cultivated species. Gathering serves multiple purposes with the overarching theme of addressing community health needs in the form of preventative care, quality control of ingredients, and surplus in times of scarcity. Although Hutsulshchyna is split between two countries, certain medicinal species uses transcend borders, grounding TEK to place.

Shared medicinal uses include 13 species that are noted in the top 20 species of cultural importance according to indices explored. Additionally, there were 35 unique place-based plants and corresponding uses that ground this TEK in the cultural, historic center of Hutsulshchyna, Verkhovyna. Lastly, unique to this study, “ecological use” was created as an attempt to integrate TEK into quantitative ethnobotanical indices, failing to capture both the depth and richness of knowledge. TEK is explored through qualitative methods including participant observation and community-based participatory action research, elucidating meaning to the role of place, phenology and gathering methods present in Hutsulshchyna. The range of accessibility to habitats in forest-dependent communities is imperative especially if it serves as a relational thread to food, medicine, and ecological grounding in cultural practice. Future indices acknowledging the variance of accessibility in today’s rapidly environmentally changing world could inform broader policy initiatives. Acknowledging TEK goes beyond the use of the organism and acknowledges derivation of place that sustains the stewardship and future accessibility of species . It is the link between use, stewardship, and culture. Typical agricultural systems attempt to control extraneous variables in order to maximize output of yield, while traditional management cultures are based on a locally-based, small-scale approaches that center interaction with natural components of the environment. The resulting system is an ethnoecosystem that embeds management as a relationship between environmental interactions and cultural practice. Current regional ecosystem challenges like illegal logging, commercial harvesting, and climate change, as well as the ripple effects of historical, colonial, environmental practices, continue to impact gathering practices and conservation status of endangered culturally important plants and their habitats. If a plant is culturally important, then the habitat or ecosystem in which it grows is by extension important. The Hutsul cultural practice of maintaining polonynas, a culturally important ecosystem, is declining,cannabis indoor greenhouse and with it the survival of plant and lichen communities that are conjoined in song, celebration, use, and cultural importance. By contextualizing the cultural importance of plants, lichens and mushrooms into their broader ecology and relationality with communities, we can learn to create meaningful stewardship policies that directly address ecosystem challenges and prioritize conservation measures. Climate change impacts, including but not limited to extreme weather events, the rise of global temperatures, and pandemic zoonotic diseases , remind us of our interconnectedness with our local and global ecosystems. With impacts not evenly distributed across the globe but felt more drastically over land, the poles, and more arid regions , areas already experiencing food insecurity will be hit hardest. Growing challenges, such as competition for finite resources including accessible, arable land minerals, water, and energy along with current, global, environmental, and economic changes are already impacting food production in response to climate change. This reality deserves attention and thoughtful, mindful action, especially for marginalized communities worldwide, specifically Indigenous Peoples and underrepresented ethnic groups, who may experience these impacts more immediately. Many Indigenous and underrepresented ethnic communities are both societally and spatially marginalized, living in edged biomes near forests, oceans, and deserts. According to the World Bank , these same communities steward an estimated 80% of the world’s remaining biodiversity. Additionally, they are over represented among the world’s poorest, most destitute, and illiterate populations, as well as those displaced or threatened by environmental encroachment, wars, disasters, and socio-political stressors . Yet, many of these communities still survive and thrive, with resilience. In this case study, Hutsul communities, an ethnographic group of traditional pastoral highlanders in the eastern Carpathian Mountains of Ukraine, exemplify a socio-ecological approach to maintaining regional food system resilience and equity. Hutsuls have survived, thrived, and adapted in the face of colonial invasions, wars, food shortages, and now synergistic impacts of climate change and illegal timber harvest causing an increase of flooding events. Many Hutsul communities in the Carpathian Mountains are guided by traditional ecological knowledge in their day-to-day lives. Lived and experienced by local and Indigenous communities worldwide, TEK is cultural, spiritual, intergenerational, dynamic, place-based, environmental knowledge and wisdom; TEK, as a living knowledge base, is revisited, reinterpreted, and reevaluated consistently . TEK, the scientific method brought to life through culture, plays a significant role in meeting community needs, while adapting to environmental changes.

TEK serves as the foundational base for ensuring resilience in communities. TEK is built upon personal stories, past traumas, innovations, and current realities to inform contextually driven, resilient responses that are aligned with community needs. The path to achieving food security has a socio-ecological foundation, one that grafts community needs with a resilient, ecologically-grounded approach known as food sovereignty. Food sovereignty, as a term, can be controversial in its various meanings and origins . Here, we refer to the definition stated in the Declaration of Nyeleni of the Forum of Food Sovereignty in 2007. “Food sovereignty is the right of peoples to healthy and culturally appropriate food produced through ecologically sound and sustainable methods, and the right to define their own food and agriculture systems.” Within this definition emerges a powerful recognition of self-determination in how food is grown, managed and sourced. In addition, it affirms that socio-ecological relationships, rooted in sustainability, are central to this type of food system. Lastly, it states that access to healthy environments and culturally important foods are inextricably linked. Food sovereignty is not an endpoint in achieving food security; rather, it is an ongoing, adaptive capacity for a community to overcome food system threats. Adaptive capacity includes both coping mechanisms and adaptive strategies . Referring to terms commonly used in developmental studies and anthropology , coping mechanisms are short-term, quickly implemented strategies to situations that threaten livelihoods. Conversely, adaptive strategies are long-term changes implemented by communities, modifying local rules, institutions, and productive activities to ensure livelihoods. Coping mechanisms tend to emerge on individual or household levels, while adaptive strategies tend to emerge on community levels. Both coping mechanisms and adaptive strategies exist across temporal scales, whereby over time, coping mechanisms can become adaptive strategies . Through semi-structured interviews, participant observation, and long-term community based participatory action research , we identify 108 culturally important species and distinct regional environmental changes with Hutsul elders, knowledge holders, foresters, and experts. By combining quantitative ethnobotanical approaches examining species use with more in-depth qualitative approaches, we identify short-term and long term responses to regional, environmental changes resulting in the maintenance of traditional foods in Hutsul communities.

Cultural world views can be viewed on a continuum from more individuated to more integrated world views. In an individuated worldview, private, compartmentalized individual, linear, mind based, and contextually independent conception of the world is valued and common, while in a culturally integrated world view, an interconnected, reflective, cyclical or seasonal, mind/body/spirit/heart-based, contextually dependent conception of the world is valued and common . Using the framework outlined in Chávez’s work in education, I draw upon cultural continua seen in individuated and integrated cultural world views developed by the authors, specifically purpose of learning, ways of taking in and processing knowledge, interconnectedness of what is being learned and time. In many ways, the academic world in U.S. culture operates in an individuated framework, where the purpose of learning , underpinned by individual competence, drives goals, and said betterment of humanity. The mind is seen as the primary and preferred conduit of knowledge. Additionally, U.S. college and university curricula are organized primarily in an individuated way, subjects, courses, are compartmentalized and separated. There is an assumption that learning concepts in isolation will lead to greater understanding of how these parts interact within the whole. Undergraduate education tends to be thematically siloed, without introductory seminars explicitly integrating interdisciplinary thinking; the solutions to environmental, global problems rely on knowledge and ways of thinking from a myriad of disciplines. Time is structured by linear tasks that can be measured, and punctuality is linked to respect. These elements of the individuated worldview, driven by individual competence,hydroponic racks the mind being the conduit of knowledge, and linearity of time, was one that I brought into a highly integrated culture.

Part of my education in my dissertation process was understanding and living with a more integrated view of purpose, way of taking in knowledge, seeing the interconnectedness of what is being learned and a relationship-based understanding of time. Identifying with both individuated and integrated cultures, I felt conflicted by the time constraints and individuated view of the research process, especially in regard to time. It was the moment that I incorporated a more integrated worldview that my research became more collaborative, empathetic, and community-driven. A glimpse of an integrated worldview in Hutsulshchyna means that the purpose of learning is based on collective wisdom, for the betterment of the relationships surrounding us – family, community and beyond. The mind, spirit, and body as well as relationships and emotions are important ways of sensing the world and sources of knowledge. Context, connectedness, and belief that understanding how things impact one another within the community will facilitate further connection are central to community life. An example of this integrated worldview, not only present in Hutsulshchyna but also present in Ukraine is toloka, with one of the many definitions defined as collective mutual assistance . Its practice dates to the time of Kievan Rus , and writings show its definitive presence between the 17th -19th centuries . Toloka is a customary way of providing collective mutual assistance typically seen in villages. It is a moral and ethical norm, and usually done by a voluntary group of people for a community member. Its purpose is to provide rapid implementation such as harvesting a plant, building a house, joint grazing of livestock or work for the community. Community members understand that each of them can help themselves only by helping other members of the community. Knowledge creation, in the translational approach, is an iterative process that generates methods to build resilience and practical solutions for all entities engaged in the research process.

Within the broad field of ecology and environmental science, there is a rising acknowledgement of a common practice called ‘parachute science’ in which international scientists or researchers from high-income countries conduct scientific research in lower-income countries, without engaging with local communities or investing in local capacity . Bibliographic analysis of coral reef biodiversity research of scholarly articles in Scopus from 1969-2020, showed that 40% of publications with fieldwork conducted in the Philippines or Indonesia had no local scientist included . ‘Parachute science’ is driven by “outsiders” assumptions, motives, and personal needs, leading to an unfavorable power imbalance between those from the outside and those on the ground” . A translational approach specifically within the dimension of knowledge creation attempts to remedy the negative impacts and structural imbalances of parachute science. Throughout my dissertation process while living in the Carpathian Mountain region, I had opportunities to meet and develop relationships with local community members to aid in knowledge creation. Recalling back to the policy actions taken, co-authorship on a manuscript also serves as way to guide local knowledge creation. Its contribution will be evident in local policy actions spearheaded by co-authors, Maria Pasailiuk and Oleh Pohribnyi in Hutsulshchyna. One of the main goals as a result of this dissertation is its translation to a language and format that serves community members following the CARE principles3 for Indigenous Data Governance . Based on collaborative knowledge curation efforts, my goal is to co-create with Hutsul communities an ethnobotanical database within the culturally traditional Hutsul region, highlighting TEK practices of gathering and managing culturally important species . The co-created eco-cultural reservoir could be based in the Hutsulshchyna Museum in Verkhovyna, and comes at a historically important time, especially as other Indigenous groups in Ukraine are currently facing exile, cultural loss, and religious persecution from a historically colonial neighbor, Russia.

This database will serve as a critical, living knowledge base that documents the ongoing importance of culturally important species for numerous stakeholders including Hutsuls, ecologists, climate adaptation scientists, plant geneticists, linguists, anthropologists, conservationists, and community developers. It is known that the threat of bio-cultural diversity is impending, and the task goes beyond simply creating an inventory of species. Language plays a critical role in maintaining eco-cultural memory. We will document the Hutsul dialect not only focusing on local names but also the descriptive natural-history knowledge . My intent is to focus on endangered endemic species and species that have culturally influenced abundances and distributions. Through this documentation, my hope is to engage, empower and support local communities through biodiversity documentation and stewardship through culturally driven intergenerational learning, using native language-based initiatives . Knowledge creation with community members resulted in the making of a phenological gathering calendar. One of the places where I spent a lot of time was the Kryvorivnia Village Library with head librarian, Katya Yurnyuk. It was with her kindness and guidance that I was invited to go out on gathering trips with a local Women’s Collective . It was during these continual and seasonal gathering trips that I learned more about gathering practices, and various ethnobotanical uses. Additionally, it was through these gathering trips that the inception of a phenological calendar arose. This calendar, still a work in progress, will go through many iterations with community members before dissemination. With threats of climate change noted by community members, this phenological calendar would ground TEK through a community created calendar and serve as a reference point to note phenological changes over time. Facilitating local knowledge creation and curation can empower communities to direct where, in what way, and how knowledge is nested within their communities.Personal actions include lifestyle choices, advocacy, leadership, and role modeling, which can help nurture a culture of resilience . Ecologists can provide leadership and support in areas of funding, subject matter expertise, and transparent dialogue with various stakeholders through science outreach. These individual actions are personal and vary among individuals. For some ecologists, activism and advocacy may be important, forging community resilience to catalyze policy changes, relationship-building, and healing capacity. For others, science outreach and grant writing may be pivotal, providing accessibility of information and resources needed to implement policy changes. The goal is to ensure individual mobility and agency to promote resilience building within communities from a local to global scale.As an interdisciplinary ecologist, I found that access to resources stand as a common barrier facing Ukrainian scientists and researchers. Having funding to implement community projects is difficult. Leveraging my positionality, I strived to secure grants as a way of supporting community driven project ideas. Granting agencies that uphold and ask for intentional, collaborative,microgreen grow rack international work such as Fulbright scholar award and National Geographic grant have provided capabilities to partner with educational and government institutions. By securing a Fulbright scholar award and a National Geographic grant, I can help fund a proposal surrounding the ecocultural restoration of the Stone Pine in the Carpathian Mountains. Both grant agencies will provide research funds that can directly go to the implementation of this project. Vasyl Zayachuk, professor at the Ukrainian National Forestry University and Oleh Pohribnyi, forest scientist at the Hutsulshchyna National Nature Park and head of the NGO, “The Heritage of Hutsulshchyna”, proposed the idea of this ecocultural restoration project. This ecocultural restoration project attempts to address harmful colonial legacies that impacted eco-cultural practices within Hutsulshchyna.

The Austrian-Hungarian Empire, one of many historical colonizers in this region, implemented forestry practices that have negatively impacted the populations of ecologically important and vulnerable species like the Stone pine . Unfortunately, the result of destructive forest management practices of the Austrian-Hungarian Empire is still reverberating today and negatively impacting the forests of Hutsul communities. The Stone pine serves an important ecosystem protection function on mountainsides in addition to being a culturally important species to Hutsuls. This ecocultural restoration project would: 1) expand and strengthen existing monitoring of the endangered Stone pine, 2) support the development of an ecosystem service assessment of the Stone pine habitat, and 3) provide a platform for the development of a Stone pine plantation for ecocultural use. This interdisciplinary approach interweaves both quantitative and qualitative methods that will promote resilience of this diverse socio-ecological system in the Carpathians. It was the time investment that nurtured the development of these relationships that laid the foundation for future collaborative endeavors which could lead to impactful action , as seen with the Stone pine ecocultural restoration project. Securing funding is one of many direct, personal actions that ecologists can take; Others include serving as a subject expert, being an advocate and activist for resilience building within communities as well as taking leadership roles in science outreach . In many ways, the successful completion of my dissertation work took on a translational approach due to the self-evident need for self-education, transparent communication and collaboration, application to policy, integration of community-driven knowledge creation, as well as continual self-reflection on my own individual, personal actions. Self-education and consistent communication and engagement took on larger roles initially than the other dimensions of policy, knowledge curation and individual action. In many ways, it was those two dimensions that helped inform the other dimensions specifically policy as well as knowledge creation. Time constraints remain a common barrier for conducting translational research. The initial four months of self-education along with communication and engagement were a result of an unseen delay in obtaining my resident visa, which in many ways forced me to stay close to a major city. With that delay, I felt the time constraint of my one-year grant and an urgent necessity to begin the formal research process. Nonetheless, I was able to take short trips to the Carpathian Mountain region, reach out to various people and create networks which served valuable in the integrative process of engagement. While I was unaware at the time, this perceived delay ultimately served as a necessary part of the translational approach needed for this collaborative, time intensive work. In total, I spent a total of a year and half over the course of three years, living and engaging with community members. While this is significant, given the scope of work that is still left to do, it is also all-too-brief. Another challenge encountered was the struggle to measure the success of this approach . Co-publication of an article has taken a long time, with the hope that this research is identifying knowledge gaps and resulting in actionable outcomes through regional policy development. Currently, it is preemptive to say what the actionable results will be of the publication and its impact on policy integration in the region. This is the first publication of its kind voicing Hutsul perspective and as Wall et al. suggest, intentional steps taken along the way of the translational approach may be viewed as indicators to eventual success.

The experience of transitioning pear and apple orchards to wine grapes — the current paradigm of cash crop transition in this region — stands to be a useful parallel for policymakers when considering the range of possible changes that may occur within the landscape, community and economy as the potential result of further transitioning farmland to growing cannabis. Broader changes that resulted from the precedent of grapes, whether positive or negative, may stand to be a sobering example to consider for policymakers hoping primarily for economic gains to be had from expanded cannabis cultivation. As several interviewees mentioned, cannabis does not fall under Right to Farm laws, or laws that protect the right to conduct standard agricultural practices in a community. This highlights a key shortcoming of the cannabis and wine grapes analogy, as the expansion of the legal cannabis industry is not afforded the same rights as the expansion of wine grapes in an agricultural community. Nonetheless, in some areas legalization of recreational cannabis may bring with it an overhauling of the current landscape of food production, just as wine grapes did. The divide that some interviewees noted between wine producers and other food producers perhaps mirrors the divide between cannabis producers and non-cannabis producers. Interestingly, wine producers — and, as previously mentioned, the alcohol industry in general — have already made various commercial connections with cannabis producers, with collaborations ranging from a “wine and weed” conference in Sonoma in summer 2017 to cannabis-infused beer and wine. Newly developed regulations surrounding cannabis cultivation will have great significance for food producers in these counties. As they are refined,cannabis grow system it is important that policymakers continue to involve the agricultural community and intentionally incorporate their perspectives.

This should be done through outreach with individuals, organizations and food policy councils to ensure that new regulations for cannabis are not unintentionally spilling over into agriculture or otherwise compromising the diversity of producers in these counties. The interviews reported here establish a baseline knowledge of how legalized recreational cannabis intersects with agricultural communities. Future research could focus on investigating land sale and ownership data to see what land types are targeted by which type of investors in each county, and to then begin to determine what types of outcomes might be associated with these different types of investors. Meanwhile, the land situation during this transition period is fluid. In Humboldt County, after the end of the study reported here, a recent town hall meeting highlighted the economic crisis facing the community with the decline of the price of cannabis. Community members reported that property values had dropped and that large, and not small, cannabis businesses had generally been receiving the bulk of new permits . In addition to more research, a renewed effort might be made to prioritize support for farm succession planning and explore creative approaches to transitioning key pieces of farmland to the next generation of farmers who identify with the non-cannabis community. Such an initiative could bolster efforts to maintain diverse local food systems in Humboldt, Mendocino and Sonoma counties.As of February 2022, 19 states and Washington, DC, had voted to legalize recreational cannabis use in the US,1 although the US federal government classifies cannabis as a Schedule I substance with high abuse potential and no approved medical purpose despite therapeutic evidence.Canada legalized cannabis nationally in 2018,8 establishing a market based on advice from a task force including government, health, research, legal, law enforcement, and non–cannabis business representatives.

Input was solicited from advocacy organizations and cannabis companies,the latter of which seek reduced taxes and regulations.10 Research on the effects of recreational cannabis legalization on cannabis use has been inconclusive, with studies reporting different increases and decreases in consumption after legalization.One study12 found that cannabis use increased in the past year and past month among populations of Asian, Hispanic, Native American, non-Hispanic White, and Pacific Islander race and ethnicity and among individuals reporting multiple races and ethnicities who were aged 21 to 30 years in states that had legalized medical and recreational cannabis. The study found no changes in cannabis use by non-Hispanic Black individuals or for any racial or ethnic demographic group aged 12 to 20 years.Another study of cannabis consumption in Canada found increased prevalence of cannabis use among middle-aged and older adults after recreational legalization.Federal surveys conducted in 2020 by their respective governments found that 17.9% of individuals 12 years or older in the US14 and 27% of persons 16 years or older in Canada consumed cannabis in the past 12 months.Further cannabis normalization could increase consumption as well as expand the reach and strength of companies selling cannabis products. Corporate social responsibility constitutes a company’s philanthropic, ethical, and economic activities beyond profit seeking, including mitigating environmental and societal impacts.Corporate social responsibility promotes brands and secures government goodwill that protects business interests. Controversial industries, including the tobacco industry, use CSR to bolster reputations burdened by core stigma, a term indicating that products, conduct, or customers have a negative social impact. Tobacco industry CSR activities ostensibly address harms caused by its products but blame consumers and present its programs as alternatives to regulation. 

That industry’s efforts include “youth smoking prevention” programs with forbidden fruit messaging, smokers’ rights groups that resist regulation, and pharmaceuticalization, which reoriented business toward nicotine replacement therapy. The World Health Organization Framework Convention on Tobacco Control, an international treaty adopted in 2003 to steer countries’ tobacco control programming, mitigate the global tobacco epidemic, and curb the tobacco industry’s interference in regulation, defines CSR as a form of advertising. As legal cannabis sales expand to new jurisdictions, the cannabis industry may seek to use CSR to gain legitimacy, secure approval, recruit allies, access government, and influence policy as it attempts to expand its customer base and legal markets. Past research on this question is limited: searches of academic databases found only 1 book chapter covering the CSR activities conducted by 2 cannabis companies operating in Colorado and 3 articles addressing CSR practices by cannabis companies, all of which stated that further research was needed. Comparison of cannabis companies’ CSR practices with those of the tobacco industry is useful because we can assess commonalities and differences between the practices of a nascent industry exiting illegality and a long-established sector with a product that has historically been legal. The recent legalization of cannabis in Canada and patchwork legalization of cannabis in 18 states in the US has led to the appearance of multiple smaller businesses alongside the multinational corporations in this sector. Owing to differences in the legality of cannabis within the US, cannabis businesses face varying market regulations. The prior criminalization of cannabis and resulting disparate racial and social harms has generated attention regarding how cannabis companies address diversity, equity, and inclusion, potentially influencing the focus of its CSR practices relative to the tobacco industry. We reviewed cannabis CSR activities in the US and Canada between January 1, 2012, when Colorado first legalized recreational cannabis, and December 31, 2021. We sought to determine whether cannabis companies have CSR practices similar to those of the tobacco industry because both sell substances that are recreationally consumed and harmful to public health with comparable consumption modes .For this qualitative study, we reviewed documents related to the CSR activities of cannabis companies between 2012 and 2021 using established methods from previous research of the tobacco, alcohol, and food16 industries. We sampled the 10 largest cannabis companies by market capitalization as of January 2021 identified by Nasdaq, an electronic securities trading market covering the US, Canada, and Europe. We excluded 1 pharmaceutical company making cannabis derived treatments and not involved with recreational markets, leaving 9 companies in our sample. Our data collection, coding, analysis, and writing process followed the Standards for Reporting Qualitative Research reporting guideline. Our approach also relied on existing protocols for searching and analyzing industry documents. Because this study did not involve human subjects, informed consent was not applicable. This research was approved by the University of California, San Francisco, Institutional Review Board. Information regarding CSR activities was collected from August 1 through December 31, 2021, through systematic searches of cannabis company websites and Nexis Uni articles written in English. Websites were reviewed for CSR activities, including events, sponsorships, nonprofit partnerships, education initiatives, diversity, equity, and inclusion initiatives, donations, and sales drives. Information on company websites was image captured and downloaded in PDF form. We searched Nexis Uni to find press releases and news coverage of cannabis companies’ CSR activities. Search terms included company names combined with the keywords corporate social responsibility, social equity, and donate. Subsequent snowball searches, a process in which key terms are identified in initial searches to find additional information, was used with brand and program names identified in initial searches. Philanthropic activities were included even if not labeled CSR. We identified 153 unique news articles, press releases,flood table and Web pages that are described below. We performed a content analysis to categorize evidence thematically, similar to studies used previously to analyze tobacco and pharmaceutical industry activities. One author with experience coding tobacco industry documents created a master file that summarized each cannabis company’s CSR activities provided in each document. Themes were developed inductively through iterative coding and categorization of language and informational patterns found in collected materials.

Corporate social responsibility activities with language and foci designed to mitigate cannabis prohibition harms were grouped into a theme category, as were activities with language and foci regarding diversity, equity, and inclusion; charitable contributions; therapeutic and medical cannabis access promotion; and mitigation of cannabis industry harms. We found that cannabis companies used CSR practices similarly to tobacco companies, claiming they voluntarily self-regulated by limiting youth access, making charitable contributions, and supporting advocacy organizations. We categorized activities under these classifications. When a document’s relevance or categorization was questioned, it was discussed by 2 authors until agreement was reached.Seven companies claimed their CSR activities addressed harms from past cannabis prohibition . Green Thumb Industries Inc and Cresco Labs Inc created business incubators and licensing assistance programs for members of racial and ethnic minority populations and communities harmed by cannabis prohibition. The License Education Assistance Program, launched by Green Thumb Industries Inc in 2019, reoriented programming to support 3 social equity license applicants in Illinois102 in August 2021. The Social Equity and Education Development business incubator developed by Cresco Labs Inc held 13 events between 2019 and 2020 that assisted 225 applicants pursuing Illinois retail licenses. Curaleaf Holdings Inc and Green Thumb Industries Inc funded nonprofits helping people with cannabis-related records rejoin society. Curaleaf Holdings Inc donated 10% of proceeds from BNoble cannabis product sales to 5 organizations helping formerly incarcerated people obtain work. Green Thumb Industries Inc allotted a portion of Good Green sales to a nonprofit grant program funding cannabis education, work training, employment assistance, and expungement services in communities harmed by cannabis criminalization. Green Thumb Industries Inc claimed it provided 3 Good Green grants as of 2021. Green Thumb Industries Inc collaborated with the Last Prisoner Project, a nonprofit founded by cannabis entrepreneurs dedicated to expunging sentences of formerly incarcerated people and reintegrating them. Three companies, Curaleaf Holdings Inc, Cresco Labs Inc, and Green Thumb Industries Inc, established quotas and initiatives to hire previously incarcerated people. Cresco Labs Inc and Green Thumb Industries Inc90 developed restorative justice CSR activities. Cresco Labs Inc established scholarship programs at 2 universities in Ohio for people or communities harmed by drug war policies. Green Thumb Industries Inc funded 4 scholarships in 2 Ohio schools for students seeking cannabis industry involvement. Canopy Growth Corporation, Curaleaf Holdings Inc, Green Thumb Industries Inc,81-84 Cresco Labs Inc, and Trulieve announced intentions to support efforts and organizations seeking to expunge criminal records. Six companies joined industry associations supporting restorative criminal justice: Trulieve andCresco Labs Inc joined National Cannabis Roundtable, a US trade organization, whereas Canopy Growth Corporation, Curaleaf Holdings Inc, Cronos Group Inc, and Columbia Care joined the US Cannabis Council, a coalition seeking to end federal cannabis prohibition. Between 2019 and 2020, Cresco Labs Inc claimed it spent nearly $425 000 in staff hours and contributions toward restorative justice, including more than 90 hours of staffing at expungement events, sponsorship of 8 expungement events and 1 gun exchange, and assisting restorative justice activations in California, Illinois, and Pennsylvania.

The implementation of medical marijuana policies did not have any significant associations with hospitalizations related to marijuana dependence or abuse. However, it was associated with a 23% reduction in hospitalizations related to opioid dependence or abuse and a 13% reduction in hospitalizations related to OPR overdose . In Table 2, the first column for each outcome variable evaluates the indicator of medical marijuana dispensaries. Relative to generic implementation of medical marijuana legalization, the operation of medical marijuana dispensaries had comparable associations with hospitalizations related to opioid dependence or abuse and OPR overdose . The second column for each outcome variable reports results including both the indicator of medical marijuana policy and the indicator of medical marijuana dispensaries. Medical marijuana dispensaries alone did not have any independent associations with any hospitalization outcomes after indicators for medical marijuana policy implementation were also included in the regressions. In Table 3, we explored if any policy effects could be detected in the periods prior to the implementation year of medical marijuana policies. We found no evidence that hospitalization rates of any category differed between states adopting and non-adopting medical marijuana policies in the pre-policy periods. Table 3 also assesses the presence of dynamic policy effects after the implementation year. We found that the reduction in hospitalizations related to opioid dependence or abuse was most salient after 1 year of policy implementation , whereas the reduction in hospitalizations related to OPR overdose was observed in the third year after policy implementation . With respect to other policy and socioeconomic covariates, uninsured rate was associated with increased OPR overdose hospitalizations. Other covariates including marijuana decriminalization,weed trimming tray prescription drug monitoring program, and pain management clinic regulations were generally not associated with any hospitalization outcomes.

Using state-level administrative hospitalization data during 1997–2014, we found no convincing evidence that the implementation of medical marijuana policies was associated with a subsequent increase in marijuana-related hospitalizations. This result was robust to the key policy dates defined in different ways. In conjunction with the studies that demonstrated negative or null associations of medical marijuana policies to substance abuse treatment admissions , suicide rates , and crime rates , our study counters the arguments about the severe health consequences that legalizing medical marijuana may bring to the public health. It should be noted that this study does not necessarily contradict some prior research that reported an increase in marijuana use prevalence in association with medical marijuana policies . It just appears that, even if legalization resulted in an increase in the prevalence, it did not contribute to the severe health consequences that concern the public the most. Whether such findings hold in the long term needs further monitoring and investigations. This study demonstrated significant reductions in OPR-related hospitalizations associated with the implementation of medical marijuana policies. These findings were supported by the recent studies that reported reduced prescription medications , OPR overdose mortality , opioid positivity among young and middle aged fatally injured drivers , and substance abuse treatment admissions in association with medical marijuana legalization. The mechanisms for the causal connections between marijuana and OPR are not clear. As mentioned earlier, using marijuana can lead to either an increase or a reduction in OPR use depending on the use purposes and the underlying assumptions. This study appears to support the hypothesis that patients prescribed with OPR substitute OPR with marijuana, but it is not directly testable in our data. An alternative explanation for the results reported in this study is that states with medical marijuana legalization may also have tough OPR prescription regulations.

However, this hypothesis was not supported by the null associations of OPR prescription regulations estimated in this study. Future empirical evaluations are warranted to explore the use pattern of OPR and marijuana and substantiate the substituting and gateway effects of the two drugs. Consistent with prior research , policy effects reported in this study were not static. We found reductions in OPR-related hospitalizations immediately after the year of policy implementation as well as delayed reductions in the third post-policy year. Nonetheless, the availability of medical marijuana dispensaries was not independently associated with hospitalizations as suggested by other studies . A possible interpretation is that only 1 state in our data legalized medical marijuana but did not have operating medical marijuana dispensaries; a few other states opened medical marijuana dispensaries within only 1–2 years after the legalization of medical marijuana. The lack of variations in policy adoption and timing limited our ability to detect independent effects of detailed policy provisions of medical marijuana legalization. The 300% increase in hospitalization rates related to marijuana is striking. In contrast, the past-month prevalence of marijuana use increased at a much slower rate from 6% in 2002 to 7.5% in 2013 . It is unclear what factors have been driving the huge discrepancies between the trends of use prevalence and the trends of hospitalization rates. Although quite a few states legalized medical marijuana or decriminalized marijuana, this study suggested that they did not contribute to the rise of marijuana-related hospitalizations. One alternative hypothesis is the escalation in marijuana potency , which has tripled from 4% in 1995 to 12% in 2014 in the U.S. . Nonetheless, empirical evidence again did not find any associations between the potency increase and the legalization of medical marijuana . Studies to understand the growing market share of high-potency marijuana and its associations with marijuana-related hospitalizations are urgently needed. The unprecedented increase in OPR-related hospitalization rates and other related health outcomes has become a major public health crisis.

Compared to the limited research on marijuana, OPR abuse and overdose epidemic has been relatively well studied. Despite lack of evidence in this study, prescription drug monitoring programs and pain management clinic regulations have shown promises to tackle the OPR crisis in some other studies . If the causal relationship indicated in this study can be substantiated in future research, medical marijuana legalization and regulation may be considered as an alternative strategy to reduce OPR-related hospitalizations without aggravating the adverse consequences related to marijuana. Our study was subject to several limitations, most of which were related to the data used. First, some states included hospitalization records in the SID from non-community hospitals such as psychiatric facilities and Veterans Affairs hospitals, but some states did not . States may also vary on ICD-9-CM coding practice particularly for drug dependence, abuse, and overdose cases. The coding of opioid dependence or abuse may include heroin cases. The inclusion of state fixed effects should to some extent alleviate these biases in the reporting. Second, the aggregate SID data represented the total number of discharges but not the total number of patients because a patient may be admitted to hospital more than once in a year. The public-use SID were not available before 1997 and not all states participated in the SID during the study period. The findings may not be generalizable to the states that were excluded from this study. Particularly, the results may be inapplicable to California, which has the longest history of medical marijuana legalization as well as the largest population of registered medical marijuana patients and the largest number of medical marijuana dispensaries. Third, although no statistical differences in hospitalization rates between states adopting and non-adopting medical marijuana policies were revealed before policy implementation,cannabis grow setup we cannot rule out policy endogeneity issues that may be caused by time-varying unobserved factors and were not captured by the two-way fixed effects models. In addition, we were not able to examine detailed policy provisions of medical marijuana legalization such as home cultivation and requirement of patient registry because of small sample size and lack of variations. We were not able to assess OPR-related policies that were adopted by a few states most recently, such as requirements of following OPR prescribing guidelines and mandatory checking prescription drug monitoring program data by providers. This limitation, however, is unlikely to influence the study findings significantly because these policies were not adopted until the very end of the study period or after the study period. Finally, the study findings do not apply to recreational marijuana legalization. In fact, the findings are likely to alter if marijuana for recreational purpose is indeed a gateway drug to OPR. Examinations on the most recent regulations of recreational marijuana are warranted.The United Nations recently estimated that the global illegal drug trade is worth at least US$350 billion annually, and illegal drug use remains a major threat to community health and safety.In addition to the range of harm associated with the direct health effects of drugs, including fatal overdose, illegal drug use is also one of the key global drivers of blood-borne disease transmission, in particular HIV infection. Illegal drug markets also contribute to community concerns, such as high rates of violence in settings where the trade proliferates.

In response to the health and social concerns associated with illegal drug use, several UN conventions were organised to control the possession, consumption and manufacture of illegal drugs.As a result, during the last several decades, most national drug control strategies have prioritised drug law enforcement interventions to reduce drug supply, despite recent calls by experts to explore alternative models of drug control, such as systems of drug decriminalisation and legal regulation. Some unintended consequences of this approach, such as record incarceration rates, have been well documented. In addition, a small number of studies assessing aspects of drug supply, measured through indicators of drug price, purity/potency and seizures, have been undertaken to describe the global relationship between these indicators over the long term. However, systematic evaluation of these relationships is still needed to elucidate patterns of drug supply. The present study, therefore, sought to systematically identify international data from publicly available illegal drug surveillance systems to assess long-term estimates of illegal drug supply.The primary outcomes of interest were long-term patterns of illegal drug supply, measured through indicators of price and purity/potency for three major illegal drugs: cannabis, cocaine and opiates . While data on amphetamine-type stimulants exist in some countries , this class of drugs was not included given inconsistent data collection and classification, and fluctuating surveillance periods and overall data quality. A secondary outcome of interest was data on illegal drug seizures in major illegal drug source regions and, major destination markets, as identified by the United Nations Office on Drugs and Crime . These secondary outcome data were used as an additional proxy measure to assess the availability of illegal drugs in specific regions, as has been carried out previously. All outcomes were systematically identified through publicly available illegal drug surveillance systems. Linear-by-linear association trend tests were carried out on annual estimates of all outcomes of interest. Price and purity estimates represent median values for each year, while estimates for seizures represent crude totals of quantity seized. All price estimates are expressed in 2011 USD and are, where possible, adjusted for purity. An online search of surveillance systems monitoring illegal drugs using two a priori defined inclusion criteria was carried out. Search terms included the following: drugs, illicit, illegal, price, purity, potency, surveillance system, government data, longitudinal, annual, estimate. Inclusion/exclusion criteria were as follows: only surveillance systems that included continuous longitudinal assessments of these outcomes of interest for at least 10 years were included because we specifically sought to assess the long-term impact of enforcement-based supply reduction strategies on illegal drug price and purity/ potency. Finally, data extraction was restricted to 1990 and onwards to focus on patterns of supply during recent decades. Data were obtained through online searches of registries of surveillance systems , governmental reports and peer-reviewed publications, through referrals from experts in the field, and through data requests to relevant organisations including the UNODC. All authors had complete access to all data and all had final responsibility to submit for publication. Ethics approval was not required given that we relied exclusively on publicly available data.We identified seven government surveillance systems that met inclusion criteria. Of these, 3 reported on international data, 3 on data from the USA and 1 on data from Australia. One of the longest running surveillance system identified, the US-based Marijuana Potency Monitoring Project, is funded by the US National Institutes of Health and was established in 1975, while the most recent surveillance system was established in 2001 .