Monthly Archives: November 2024

The iceberg was not a single event but a series of events so the southerly flow was effectively blocked for a decade

We believe that this occurred in the early 2000s although it could have started after 1989 when we last visited the structures. We lack information on the most obvious and interesting observations: the mode of reproduction, the settlement biology, and the growth of this interesting sponge. The obvious questions relate to the explanation of the event. We have no knowledge of the actual propagules or the settlement, only recruitment to a size that can be seen and identified. There are no published descriptions of dispersal propagules of A. joubini, their settlement preferences, or their growth rates. We have seen very small buds that we assume are asexually produced by another hexactinellid, R. antarctica, and we have collected them in the water column in strong currents. Thus, we know that asexually produced buds can move through the water column where they could in principle be entrained and lifted by strong tidal currents; however, we have not seen R. antarctica or any other hexactinellid beside A. joubini on any of our settling surfaces. To our knowledge, there is no evidence of any Antarctic hexactinellid sponge demonstrating sexual reproduction, although it has been seen elsewhere. In our cases A. joubini propagules must have been abundant, at least around the gangplank on Ross Island and at Explorers Cove where there was massive recruitment high in the water column. Given the heavy recruitment observed on artificial surfaces well above the seafloor, we suggest that swimming larvae are released episodically. Why is the A. joubini recruitment predominantly on artificial surfaces? We have no data to address this interesting question, equipment for growing weed but we hypothesize that there are more predators on natural substrata and that these predators serve as a strong filter on the survivorship of the propagules as discussed by Thorson.

Oliver and Slattery offer strong evidence of the efficiency of a microcanopy of carnivorous invertebrates near the gangplank, and Suhr et al. demonstrated that three of the most common foraminifera, especially Astrammina rara, consume metazoa including planktonic invertebrates in Explorers Cove. Out of this, it is reasonable to speculate that benthic predation filters settling larvae as discussed by Thorson. Another obvious question relates to the fact that we saw no measurable growth of many naturally occurring A. joubini between 1967 and 1989, yet beginning sometime between then and 2004 they exhibited tremendous growth. With the exception of two small sponges, none of the structures had any A. joubini in 1989. However, in 2004 these structures were photographed with very large sponges that presumably had settled after 1998, but certainly no earlier than 1990 , and by 2010 sponges had obtained diameters ranging from 7 to 72 cm . Further, the estimated mass of a sponge observed on an artificial substrate at Cape Armitage in 2010 increased about 30% when it was re-photographed in 2012. Clearly, rapid growth rates are possible by A. joubini. What environmental factors were responsible for this sudden growth? The most likely correlate with the growth if not the settlement was a probable shift in plankton composition. Typically the transport of abundant primary production from the north results in a seasonal plankton bloom composed of relatively large phytoplankton . However, in the 2000s a series of large icebergs were grounded, blocking this transport and preventing the annual ice from breaking up and going out until 2011. The icebergs and thick sea-ice probably interfered with the advection and growth of the large phytoplankters that usually dominate in the water column. Thrush and Cummings and Conlan et al. summarized many populations that were negatively impacted by the lack of advected primary production over this decade.

The dynamics of A. joubini were also correlated with this phenomenon, and we suggest that changes in the plankton may have resulted in a shift from large phytoplankters to tiny dinoflagellates and bacteria. Margalef postulated such a relationship in water columns to result from reduced resources. Sea ice thickness and transparency affects benthic productivity and ecosystem function. Montes-Hugo et al. , described such regional changes in the Western Antarctic Peninsula suggesting a strong relationship between ice cover and the size of the phytoplankton. Orejas et al. and Thurber 2007 discuss the strong relationship between microplankton and Antarctic sponges. Reiswig and Yahel et al. , working on other hexactinellid sponges, demonstrated that they retain only very small particles of bacteria and protists. As hexactinellids in general seem restricted to feeding on tiny particles, the shift in plankters may have offered a strong pulse of appropriate food for A. joubini, triggering rapid growth that was previously not observed in this species. Moreover, our observations of relatively fast growth following a shift in the food is supported by Kahn et al. who report relatively fast temporal changes in the density of two deepwater hexactinellid sponge species in 4,000 m depth off Monterey, California, USA. These density shifts occur with a lag of 1–2 years following shifts in the food supply of the micro-particles they consume. Although A. joubini growing on the gangplank had a broader weight distribution than the same species growing on the floaters in Explorers Cove , we are hesitant to attribute these differences to the site location. It is very likely that the individual sponges that fell off the racks and floaters in Explorers Cove were larger than the sponges that remained on these substrata. Therefore, the measurements from these two substrata at Explorers Cove could be skewed to smaller-sized individuals. We also have preliminary but convincing evidence of A. joubini mortality.

Although we were not able to relocate all transects in 2010 and therefore may have missed some surviving sponges, at least 67 large A. joubini died in the 40 years of this program with no known survivors. We have no reason to question earlier observations that some mortality results from predation by A. conspicuous and the amphipod S. antarctica. Additionally, Cerrano et al., report patches of diatoms inside A. joubini, but speculate that the diatoms had invaded and are detrimental to the sponges. We agree and have seen the amphipod, S. antarctica, eating patches of the sponge that subsequently are colonized by diatoms. In 2012 we photographed considerable evidence of incipient amphipod infestation on A. joubini at the gangplank; however, the actual mortality sources within this study are not known and some may reflect ice formation on the sponge that kills the tissue in a patchy manner, later becoming infected with S. antarctica. We emphasize that many of these large A. joubini surely do live longer, and we are only considering sponges in our localized study sites, but this is still a very high mortality rate for a species of sponge thought to be long-lived. Summarizing the A. joubini observations of massive recruitment and growth and rapid mortality, we suggest that this sponge has much more dynamic life history than previously suspected. What of the other Hexactinellida in our study sites? We know that R. antarctica grows relatively fast as this was studied in the 1970s. We observed surprisingly fast growth and asexual reproduction of mature individuals and we also observed some 40 very small R. antarctica buds to increase their volume as much as two orders of magnitude . This species is by far the dominant sponge in the 25–50 m depth range at McMurdo Station, but it is so inconspicuous that it is extremely difficult to evaluate the population patterns. Obviously it has the potential to multiply relatively quickly, yet we have no evidence of sufficient mortality to balance the reproduction and growth rates observed. The other common Antarctic hexactinellid is R. nuda/racovitzae. This knobby, volcano-shaped sponge is smaller than A. joubini and remains an enigma with regard to its population dynamics and growth rate. Prior to the removal of the cages in 1977, seven R. racovitzae survived inside cages , while 2 died inside their cages. Those survivors did not show significant growth during that time period. The mortalities may have resulted from sea star predation or infestation of S. antarctica. Our extensive surveys in 2010 may have come across a few young R. nuda/racovitzae although they were not collected and we are not sure of their identification. It is interesting to note that Fallon et al., report a relatively-small, 15 cm diameter specimen from the Ross Sea was approximately 440 years old. Many of the R. racovitzae in our area were at least a meter tall, so this species might obtain great age. Rossella fibulata is a rare sponge in the McMurdo Sound area; however, two individuals settled on a rack at Explorers Cove and on a cage at Cape Armitage. It appears to grow rapidly but otherwise little is known of its biology. In any case, the four hexactinellid species in this shallow habitat certainly have different life history patterns, with the fast turn-over of A. joubini being the most surprising. Our observations complement those of Teixido´ et al. who report high frequencies of asexual reproductive strategies in three deep-water Hexactinellida in which 35% of the observed R. nuda were actively budding. In addition, grow tables 4×8 many R. racovitzae exhibited reproduction by fragmentation while R. vanhoeffeni reproduced with bipartition. Thus, it appears that each of the Antarctic Hexactinellida species exhibits different life history biology.

In summary, these observations allow us to test and reject the prevailing notion of slow rate processes for both recruitment and growth of A. joubini. The population dynamics imply that A. joubini are fast to respond to an environmental shift, but the population increase may be relatively short and we need to re-evaluate ideas of slow processes and stability over century time scales. These surprising results are set in a time of climate- and fishing-related environmental changes. Certainly these results demonstrate the great importance of comprehensive, long-term data sets designed to better understand such processes. Voucher specimens collected in the 1960s were sent to the Smithsonian Oceanographic Sorting Center and the specimens seem to be lost; however, a collection of specimens is available at the Scripps Invertebrate Collections.Adolescence is a critical period of development marked by the formation of self-concept and identity, independence from parental guidance, and growth in cognitive and socioemotional skills such as empathy, resilience, and creativity. However, some adolescents also begin to engage in risky behaviors, such as use of tobacco, cannabis, alcohol and other substances. These behaviors are significant, as they can negatively influence this important developmental period and contribute to a vicious cycle whereby risky behaviors interfere with school engagement and academic performance and vice versa. This negative feedback loop is suggested by Richard Jessor’s Theory of Problem Behavior, which proposes that school climate, including the social environment of peers, contributes to adverse adolescent behaviors and outcomes including school disengagement, risky behaviors, and academic failure. These adolescent behaviors in turn influence the school climate, as when groups of students normalize delinquent behaviors, undermining academic engagement more broadly. This vicious cycle in adolescence can have significant downstream effects in adulthood, potentially affecting educational and socioeconomic opportunities as well as overall health outcomes. While Jessor’s theory suggests reciprocal effects between a negative school climate and adolescent risky behaviors, it may also suggest that a positive school climate could create a virtuous cycle of improved academic success, greater school engagement, academically and prosocially supportive peers, and better academic and behavioral outcomes among teens. This is supported by prior literature which has shown that positive school climate is linked to better academic performance, student well being, and school engagement, and lower rates of problem behaviors such as disruptive, antisocial, violent, bullying, or delinquent behavior. Although there is no standardized measure of school climate, there are several domains which have been used to characterize school climate and show predictive potential, among them: the institutional environment, student-teacher relationships , and disciplinary styles. However, prior studies have primarily only examined a limited set of school climate variables and adolescent risky behaviors and most have been limited to cross-sectional designs. As a result, it is still unknown which aspects of school climate might be targeted to improve specific academic or health outcomes. The present study sought to identify and compare associations between school climate measures across multiple domains and multiple downstream health and academic outcomes longitudinally.

The thermal pathways appeared more efficient under the temperature conditions tested

In other words, the eliquid will be entirely VG well before the e-liquid reservoir is depleted. The predicted percent of e-liquid remaining at full VG enrichment in the model is fairly insensitive to starting volume in the e-liquid but is sensitive to starting PG:VG ratio and temperature, as expected. Thus, a user may be inhaling high relative concentrations of acrolein and other predominant VG products in the aerosol for a significant amount of time during the e-liquid cartridge or reservoir lifespan.The vaping process for e-cigarettes is complex and dynamic, possibly more so than currently appreciated. Coil temperature, puff duration, and PG:VG ratio all significantly affect both theaerosol production and the composition. Most of the mass that was lost from the e-liquid could be accounted for as PG and VG. Furthermore, volatile/semivolatile compounds dominated the total aerosol. Caution should be exercised when collecting particles with dense filter material or with overloaded filters for studying the particle phase, as the semivolatiles can be trapped and interpreted as particulates. In general, the chemical mechanisms for forming carbonyls appear to be well understood, and consistent with the numerous insights gained from interpreting the carbonyl mass yield as normalized by aerosol mass. Some exceptions include acetone, for which there may be a radical pathway from VG not currently accounted for, and acetaldehyde, for which there may be a thermal pathway from PG. Importantly, drying room the user’s exposure to toxic carbonyls such as acrolein may change during the vaping process, and the user may be exposed to high relative content of VG and its degradation products as the e-liquid is depleted.

These findings support the need for further research into aerosol composition and toxicology as a function of the e-cigarette puffing life cycle, in addition to e-liquid composition, puffing regimen, and vaping device operational conditions.The unexpected outbreak of e-cigarette or vaping-associated lung injury was reported nationwide starting in September 2019, causing more than 2800 hospitalizations and 60 deaths. The specific biological mechanisms of EVALI, as well as the chemical causes, are still under investigation. Emerging evidence shows that EVALI is associated with vaping tetrahydrocannabinol containing e-liquid cartridges that were obtained on the black market. Although adverse health effects of vaping THC cartridges have been found to include abdominal pain, nausea, chest pain, shortness of breath, and acute respiratory distress, they have not to date been fatal. The sudden deaths and hospitalizations from EVALI are, instead, strongly linked to a compound called vitamin E acetate , the chemically-stable esterified form of vitamin E . VEA is thought to be used as a cutting agent in THC cartridges because it has a similar viscosity to THC oil, so that the adulteration will not be visually evident. FDA labs confirmed that VEA was present in 81% of THC-containing vaping cartridges confiscated from 93 EVALI patients. VEA was also found in the bronchoalveolar fluid samples from 48 of 51 patients, but not found in samples from the healthy comparison control group. The VEA fraction in vaping cartridges confiscated from EVALI patients range from 23% – 88%. The interaction between aerosolized VEA with lung surfactant, the toxicity of VEA thermal degradation products, or other components in the vaping aerosol of extracted THC oil have been hypothesized to explain the association of VEA to EVALI. It should be noted that there is currently not sufficient evidence to rule out the contribution of other diluents, flavoring additives, pesticide residues, or other ingredients found in THC cartridges.

It’s also not known if VEA has a synergistic effect with THC oil components that may lead to EVALI. A limited number of recent research publications has focused on either the physical and chemical properties, or the biological effects of the vaping aerosol from VEA. DiPasquale et al.observed VEA was capable of reducing the elastic properties of pulmonary surfactant and thus cause lung dysfunction by alveolar collapse or atelectasis. Lanzarotta et al. found evidence for hydrogen bonding between VEA and THC in both vaping aerosol and unvaped e-liquid, suggesting they may synergistically cause EVALI. Wu et al. showed that the toxic gas ketene, as well as carcinogenic alkenes and benzene are generated from the thermal degradation of VEA. RiordanShort et al. found that pure VEA starts to decompose at an incubation temperature of 240 °C and identified over 40 kinds of thermal degradation product at an incubation temperature of 300 °C, 30 of which are carbonyls and acids. However, the experiments of Riordan-Short was done under heated headspace sampling as a surrogate vaping environment, instead of a real vaping environment in an e-cigarette tank with metal coil, where temperature gradients exist due to localized coil heating. Furthermore, different coil material and surface area will have different effects on thermal degradation chemistry. Jiang et al. reported a total of 35 toxic byproducts during the vaping of commonly used diluents including VEA; over 25 of them are carbonyl compounds. Compared to VEA, there is less research available on the vaping chemistry of THC oil extracts and other cannabinoids due to DEA regulations, even though the metabolism of THC has been well studied.Meehan-Atrash et al. hypothesized that THC emits similar thermal degradation products to terpenes given their terpenoid backbone; however, terpenes are also found in cannabis plants and can be used as additives in e-liquids, such that the degradation products may be difficult to distinguish from THC. It was also found that vaping and dabbing cannabis oil including terpenes may cause exposure to concerning degradants such as methacrolein, benzene, and methyl vinyl ketone.

Adding terpenes to THC oil led to higher levels of gas-phase products compared to vaping THC alone. Since vaping is a complex and dynamic process, a systematic understanding of the chemistry occurring during the vaping process is needed to assess potential factors that may contribute to EVALI, as well as other potential adverse health effects. In this work, a temperature controlled vaping device with accurate coil temperature measurement was used to vape e-liquids of VEA, extracted THC oil, and their mixture under typical vaping conditions consistent with the CORESTA standard. Gravimetric analysis was used to evaluate the aerosolization efficiency, while the high performance liquid chromatograph coupled with high resolution mass spectrometry was used to characterize thermal degradation products including carbonyl compounds, acids, and cannabinoids using the methods developed by Li et al. A comprehensive thermal degradation mechanism for THC and VEA are proposed, which could be useful for regulation and further research.A temperature-controlled third generation Evolv DNA 75 modular e-cigarette device with a refillable e-liquid tank and single mesh stainless steel coils was used for aerosol generation . The mod enabled variable output voltages with coil resistance of ~0.12 ohm. Evolv Escribe software was used to customize the power output in order to achieve the desired coil temperature. The coil temperatures were measured by a flexible Kapton-insulated K type thermocouple in contact with the center of the coil surface and output to a digital readout. The temperature set by the device is not truly representative of the measured coil temperature, as often, vertical farming units the device flow rate, e-liquid viscosity, and coil resistance changes will alter the relationship between applied power and output coil temperature that drives chemistry. The puff duration is 3 s with a flow rate of 1.20 ± 0.05 L/min, quantified by a primary flow calibrator , corresponding to puff volume of 60 ± 2.5 mL. The puff volume and puff duration selected in this work is consistent with e-cigarette test protocols applied to propylene glycol /vegetable glycerin based e-cigarettes.The e-liquids used for vaping in this work are: pure VEA that was used as purchased, extracted THC oil that is commercially obtained from Bio-pharmaceutical Research Company , and the mixture of the two ingredients . All THC experiments are performed at the BRC facility under an active DEA Schedule 1 license. Thecomposition analysis by gas chromatography of unvaped extracted THC oil showed that the most abundant cannabinoids are: Δ 9 -tetrahydrocannabinol , Δ9 – tetrahydrocannabinol acid and cannabigerol acid , while other cannabinoids were identified below 3% of the total peak area . Δ 8 -THC, which can be observed at 0.3 minutes after the Δ 9 isomer, was not detected in the mixture. A total of over 50% of mass in unvaped extracted THC oil remain uncharacterized, but presumably contains terpenoids and potentially other alkanes and alkenes. Three temperatures were chosen for the particle generation, with a temperature measurement deviation of 10 °F. The quantification of carbonyls is only reported at 455 °F. During the sample collection, a total of 10 puffs of aerosol with a frequency of 2 puffs/min were collected for each sample. Carbonyls, acids and cannabinoids in vaping aerosols , which represent a large portion of expected products, were collected onto 2,4- dinitrophenylhydrazine cartridges for HPLC-HRMS analysis. The consecutive sampling with three DNPH cartridges shows a collection efficiency >98.4% for carbonyl-DNPH adducts in the first cartridge. Excess DNPH is conserved in the cartridge after the collection to maximize collection efficiency.

DNPH cartridges were extracted with 2 mL of acetonitrile into autosampler vials and analyzed by HPLC-HRMS. Consecutive extractions of DNPH cartridges for samples confirmed that >97% of both DNPH and its hydrazones were extracted after the first 2 mL volume of acetonitrile. The collection efficiency for cannabinoids is unknown, since only a limited amount of THC oil was available for experiment and not for quality controlcharacterizations. The high resolution mass data of cannabinoids is only used for identification in this work. Details on the collection method are described elsewhere. Moreover, glass fiber filters were used to collect the particles, as has been done in other e-cigarette studies. The particle mass collected on filters was determined gravimetrically on a microbalance by weighing the filter mass immediately before and after puffing at different experimental conditions. The standard deviation of the gravimetric analysis after triplicate measurements was determined to be ∼20%, mainly due to variations in puffing. The sample collection and analysis were performed in triplicate.Carbonyl compounds and acids from the thermal degradation of VEA and THC were derivatized by 2,4-DNPH to form carbonyl-DNPH compounds during the collection process. The detailed mechanism and method of identification for each carbonyl were described in previous work.40 Beside DNPH adducts, HRMS has been proven to be an effective tool for the detection of cannabinoids and their oxidative products, as the phenolic hydroxyl group in cannabinoids can be ionized in both electrospray ionization positive and negative modes, while the high mass precision enables the analysis of elemental composition. Negative mode was applied for the detection in this work as both carbonyl-DNPH adducts and cannabinoids can form negative ions by deprotonation. An external mass calibration was performed using the carbonyl-DNPH standard solution immediately prior to the MS analysis, such that the mass accuracy was adjusted to be approximately 1 ppm for standard compounds, the mass calibration was then applied to a molecular formula assignment for unknown compounds. All molecular assignments were analyzed by the MIDAS v.3.21 molecular formula calculator . Carbonyl-DNPH adducts and cannabinoids in extracts solution were separated and analyzed using an Agilent 1100 HPLC with an Poroshell EC-C18 column coupled to a linear-trap-quadrupole Orbitrap mass spectrometer with an ESI source at a mass resolving power of ∼60 000 m/Δm at m/z 400. The mobile phase of LC−MS grade water with 0.1% formic acid and acetonitrile were applied in the chromatography method. The analytes were eluted over the course of 45 min at 0.27 mL/min with the following gradient program: 40% B , 50% B , 60% B , 80% B , and 40% B . After separation by chromatography, single ion chromatography of each compound were extracted for the quantification of specific carbonyl compounds based on their calibrated m/z. Formaldehyde, acetaldehyde, acetone, butyraldehyde, valeraldehyde, hexanal were quantified using the analytical carbonyl-DNPH standards. The SIC peak separation between isomers of butyraldehyde/isobutyraldehyde, valeraldehyde/isovaleraldehyde hexanal/4-methylpentanal cannot be achieved, so the concentration of all isomers were calculated as a total amount. The concentrations of glyoxal, methylglyoxal, diacetyl were calculated by an estimated ESI sensitivity as described by Li et al.40The thermal degradation of both VEA and THC was observed at the measured coil temperature of 455 ± 10 °F , which is close to temperature that VEA started to degrade in the work of Riordan-Short et al..

All analytes are baseline separated in the chromatographic spectrumusing accurate mass single-ion-chromatography

The mass concentrations of different carbonyls/acids in air were calculated by the total mass concentration of the specific carbonyls/acids in the HPLC-HRMS analysis divided by the total volume of air that flowed through the DNPH cartridge during the vaping collection process.The method reported in this work offers unambiguous identification and a large quantification range for functionalized carbonyl compounds and organic acids. This is useful for studying e-cigarette thermal degradation chemistry, as well as other environmental chemistry topics . A total of nineteen DNPH hydrazones in the e-cigarette aerosol sample were observed : five simple carbonyls, six hydroxycarbonyls, four dicarbonyls, three acids, and one phenolic carbonyl. Hydroxycarbonyls comprised 3 of the top 6 most abundant compounds. Uchiyama et al., recently found that some compounds are emitted purely as gas-phase species , some as purely particulates , and some as both . Both the concentration and phase information is useful for estimation of exposure risk. Much of the chemical identification for DNPH hydrazones can be directly derived from the exact mass of the detected [M-H]- ions alone. As the formation of DNPH hydrazones replaces only one atom , it is straightforward to deduce the original molecular formula of the carbonyl or acid from the hydrazone formula. The chemical structures were confirmed as in 2.3.1. Figure 2.6a shows the total ion chromatography and SIC of select carbonyl-DNPH compounds, Figure 2.6b shows the corresponding integrated mass spectrum of TIC and each SIC. From the TIC, it is clear that ecigarette aerosol is a complex system which contains a large number of carbonyls/acids.

Co-elution is common in the TIC ; however, the SIC isolates the chromatographic peaks of the desired m/z, avoiding co-elution and misidentification. We also found that acetone-DNPH co-eluted with vanillin-DNPH in the chromatography. This will have led to an overestimation of the abundance of acetone using a chromatography method without HRMS, as vanillin-DNPH is not commercially available.Beyond molecular formulas, it is advantageous to confirm the exact bonding sites of carbonyls and other moieties to give insight to chemical mechanisms and aid in theoretical calculations of reaction energies, as these calculations are sensitive to structures. The chemical structure of DNPH adducts was identified by their neutral and radical losses in tandem multistage mass spectrometry using collision induced dissociation , 148,149 which often helps to elucidate the exact carbon location of the moiety-of-interest for small molecules. For example, alcohols adjacent to a beta carbon with an abstractable hydrogen can lose H2O by H-shift rearrangement, 150 while those bonded to aromatic or other non-abstractable sites do not show this loss in the negative ion mode. For nitroaromatics such as DNPH, the electron-withdrawing groups of NO2 exerts a strong stabilizing effect on anion radicals, and facilitates NO2-mediated rearrangements . For small ions like acetaldehyde-DNPH, there is no other reasonable carbonyl structure that exists for the molecular formula, and MSn confirms this structure with expected fragmentation of CH3NO and CH3CHO . However, cannabis grow equipment there are some ambiguous formulas such as C3H6O3, which may belong to structural isomers dihydroxyacetone and glyceraldehyde. Both of these hydroxycarbonyls are proposed to exist in e-cigarette aerosol after NMR analysis, but are impossible to distinguish with chromatography as they have the same UV-absorption and m/z.34 With MSn fragmentation, we found that dihydroxyacetone is the main product.

Even though several fragmentation pathways for these isomers are similar and 269.05→ 239.04 , the H2O loss and C2H4O2 loss that is expected for glyceraldehyde-DNPH were observed to be negligible in the mass spectrum . The preferred formation of dihydroxyacetone over glyceraldehyde supports the radical-mediated oxidation pathways suggested by Diaz et al., as radical abstraction of the H in VG should lead preferentially to a secondary alkyl radical compared to the primary radical . The initiating radicals are suggested to be reactive oxygen species such as hydroxyl radical, and as such, the degradation products can be described by processes that occur in atmospheric chemistry. Some of the products identified here can be expected from the thermal degradation of PG and VG , which is in agreement with the proposed mechanism, while others are likely to be flavoring additives . A shared product ion after fragmentation of the DNPH hydrazones is C6H3N4O3 – , which is the modified DNPH after the O-rearrangement loss of the original carbonyl/acid. Other similar loss pathways are those of the DNPH itself, including loss of HONO, NO2, and NO . There are also distinctive fragmentation pathways for each ion, which are summarized in Table 2.2.While the process of ionization in ESI is complex, it has been demonstrated that there are key factors influencing the ionization efficiency of different compounds. For example, for the same family of compounds, there is a relationship between negative ion electrosprayionization response and pKa of the dissociation equilibrium HA ⇆ A – + H+ , which is directly related to basicity. We calculate the basicity in terms of ΔGdeprotonation , because the deprotonated [M-H]- ion is usually detected in the ESI negative mode. Our calculations of the electrostatic potential maps of carbonyl-DNPH hydrazones show that they have a primary acidic proton ; thus, they are excellent candidates for which gas phase basicity can be used to parameterize ionization efficiency in the ESI negative mode.

We emphasize that the theoretical chemistry results in this work only provide a relative indication of sensitivity, not absolute calibration factors, and only for the same family of compounds that are protonated or deprotonated. The relative theoretical sensitivities are then anchored by absolute ESI calibrations for the carbonyl-DNPH compounds where standards are commercially available.The trend of ΔGd and ESI sensitivity arises from the intrinsic relationship between deprotonation efficiency and the ability of the aromatic product ion to stabilize the negative charge initially formed on the N atom . Acrolein is the most sensitive compound in ESI negative mode because it has conjugated double bonds, i.e., additional pi orbitals for the negative charge to be delocalized. Also, ketones have lower sensitivities than aldehydes because the electron donating group on both sides of the C=N bond slightly destabilizes the negative ions. A limitation of this model occurs for compounds that have similar ΔGd. In this situation, other factors like molecular volume and polarity may also play an important role for these compounds. Despite the limitations, this method is applicable to the compounds found in e-cigarette aerosol and enables the first estimation of concentrations for complex carbonyls that have not yet been quantified with acceptable uncertainty. Furthermore, this computational technique offers an advantage compared to the time expenditure, costs, and chemical usage of synthesizing standards.The calculated concentrations of e-cigarette constituents characterized in this work are shown in Table 2.2 as mass per volume or mass per ten puffs analyzed. The most abundant compounds in the blu e-cigarette aerosol for our study conditions are hydroxyacetone, formaldehyde, acetaldehyde, lactaldehyde, acrolein, and dihydroxyacetone. While, within uncertainty, the exact order of abundance is not definitive, it is clear that hydroxycarbonyls are just as important assimple carbonyls to the composition of the e-cigarette aerosol. Hydroxyacetone has been found to be a major, sometimes dominant, emission in other e-cigarette brands and e-liquids, as quantified by gas chromatography. The agreement of the high abundance of hydroxyacetone lends support to the theoretical approach in this work, which enables all carbonyls and acids to be quantified by the same method. The high abundance of hydroxyacetone may be due to its multiple formation pathways in Scheme 2.2 and its possible role as an impurity in e-liquid, e.g., Sleiman et al., found hydroxyacetone in concentrations of < 1% of the sum of PG and VG in the e-liquids they used. We were not able to test the e-liquid in this work due to cartridge design; thus, are unable to comment on the extent of hydroxyacetone impurity in the e-liquid, if present. Dihydroxyacetone and lactaldehyde, in contrast, have not been regarded as major e-cigarette emissions until their unambiguous identification in this work. Their formation pathways from PG and VG are highly feasible, so their higher abundance is not unexpected. It’s not clear why these compounds have not been reported earlier; we suspect analytical challenges may be a reason. As we discussed previously, lactaldehyde-DNPH co-eluted with formaldehyde-DNPH in the TIC . Thus, HPLC-UV, one of most frequently used instrument for studying carbonyl compounds in e-cigarette aerosol, indoor grow cannabis will not be able to identify and quantify lactaldehyde. However, the HPLC-HRMS method overcomes co-elution challenges by distinguishing compounds based on their exact mass from the SIC and mass fragmentation patterns. Dihydroxyacetone-DNPH appeared to be baseline-separated in HPLC-UV, with a retention time slightly shorter than DNPH itself; however, its unambiguous identification is not possible without HRMS and/or authentic standards. Furthermore, both of these compounds are quite polar, and thus, not conventionally compatible with gas-chromatography.

A comparison of the absolute emission concentrations of thermal degradation products between studies is not straightforward, even for the same brand of e-cigarettes, as the puffing regimens and apparatus of reported works are all different and individual puffing parameters have non-linear effects on the thermal degradation chemistry. Klager et al., also reported high variability of carbonyl concentrations for the same brand, puffing-regimen, and flavor, suggesting that the factors driving the thermal degradation chemistry are not yet fully understood. Our work should be primarily viewed as a demonstration of a new method to the chemical characterization of our specific e-cigarette model at the stated puffing conditions, with noted insights into the thermal degradation mechanism. Formaldehyde, acetaldehyde, and acrolein are known to produce pathological and physiological effects on the respiratory tract. They are known to cause sensory irritation, inflammation, and changes in pulmonary function; formaldehyde is also carcinogenic. The average daily dose of aldehydes can be calculated by the amount of aldehydes per puff multiplied by the average number of puffs a user inhales per day. For example, the median puffs per day for e-cigarette users can be assumed to be 250171, so the average daily exposure dose of formaldehyde is 37.5 µg/day for this e-cigarette device, e-liquid, and operating conditions. The California Office of Health Hazard Assessment Chronic Reference Exposure Levels for formaldehyde is 9 µg/m3 , which could be translated to an acceptable daily dose of 180 µg/day and is higher than the e-cigarette aerosol exposure for formaldehyde in this work. In addition, OEHHA has a No Significant Risk Level recommendation of 40 µg/day which is intended to protect against cancer; this NSRL level is close to the exposure dose of formaldehyde in this work. The average exposure dose of acrolein for blue-cigarettes is 15.2 µg/day according to Table 2.2, which is higher than the OEHHA chREL value . Logue et al. used a similar approach to estimate health impacts and found that both formaldehyde and acrolein can exceed maximum daily doses derived from occupational health guidelines. Differences in results are likely due to the different devices, e-liquids, and puffing regimens used.While the reported emissions in this work may not be generalized to all e-cigarettes and use scenarios, it is informative to compare the aldehyde emissions normalized by nicotine, since ecigarette users transitioning from traditional tobacco products will self-titrate nicotine intake when using e-cigarette products. In this work, the nicotine yield is 10.4 ± 1.9 μg/10 puffs. We did not observe evidence of nicotine oxidation174 under the puffing conditions of this work, which will impact the ratio. The formaldehyde/nicotine ratio is 144 ±32 μg/mg nicotine, which is 4 times higher than the formaldehyde/nicotine ratio in combustible cigarettes . The acrolein/nicotine ratio measured in this work in close to that of tobacco products , while the acetaldehyde/nicotine ratio and propionaldehyde/nicotine ratio are lower than that in combustible cigarettes. Logue et al. observed similar trends using different e-cigarette products; however, the results were not normalized for nicotine so a direct comparison is not possible. Thus, we find e-cigarettes do not necessarily emit lower carbonyl compounds than tobacco products, but the comparisons may change depending on the specific e-cigarettes or tobacco products, or different puffing/smoking regimens. Although hydroxycarbonyls are abundant in e-cigarette aerosol, a general lack of toxicological data precludes health risk assessment. Smith et al. found that exogenous exposure to dihydroxyacetone is cytotoxic and will cause cell death by apoptosis. Glycolaldehyde is also suspected to have biological toxicity. For hydroxyacetone and lactaldehyde, toxicology data are currently unavailable on many toxicology databases like Hazardous Substances Data Bank , European Chemicals Agency and Research Institute of Fragrance Materials .

 PG and VG are known to be the major contributors to the aerosol particle phase

Underscoring the limits to garden accessibility in Milwaukee, distance to the nearest garden appears to be increasing over time. While gardens appear to be distributed in a way that makes them more accessible for marginalized groups than for more privileged ones, the gardens are becoming less accessible in general.In Seattle, Philadelphia, and Milwaukee, the primary organizations involved in building, maintaining, and defending the city’s gardens worked to gain legitimacy for themselves and, in the process, served to legitimize urban agriculture as a land use—selecting from among its many potential benefits to construct a narrative that served their organizational interests and priorities. The organizations discussed in this dissertation identified different target audiences for their legitimizing efforts, faced different challenges in gaining or maintaining legitimacy, and ultimately advanced the legitimacy of urban agriculture along different lines. As this dissertation demonstrates, variations in how urban agriculture has been legitimized have impacted the socio-natural spaces constructed in each city and the strength of arguments for long-term site preservation in the face of potential redevelopment. As it worked to gain legitimacy, Milwaukee Urban Gardens found more success as a garden support organization than it did as a land trust; as it has undertaken more programming and site maintenance over the years, Milwaukee Urban Gardens has joined with other organizations in the city to frame urban agriculture as a legitimate land use for its job training, employment and commercial potential. Urban farms are the focal point in Milwaukee, and greening is the focal point in Philadelphia, where the Pennsylvania Horticultural Society gradually evolved its Philadelphia Green program toward blight removal and neighborhood revitalization.

In recent years, this framing for the value of urban agriculture has been contested by Soil Generation, a Black- and Brown-led coalition advocating for more permanent gardens, affordable housing, and community control over land use more generally, vertical farming supplies advancing a new frame that ties urban agriculture’s legitimacy to the stewardship of longtime residents and the unjust history of dispossession they have experienced. In Seattle, the P-Patch program worked to legitimize its activities for the benefits of food production and community-building that community gardens can provide, and advocates with the P-Patch nonprofit refined this narrative over time by articulating how urban agriculture serves as a neighborhood amenity that could ease some of the strain of urban growth while attracting desirable new residents. In all three cities, economic arguments have been central to strengthening the legitimacy of urban agriculture in the eyes of city officials in order to secure more resources and favorable policy for the gardening organizations and their spaces. However, these economically focused arguments also cohere with processes perpetuating inequality in urban environments. In the case of Milwaukee’s employment emphasis and PHS’s revitalization framing, economically focused arguments have served to reinforce the conception of urban agriculture as a temporary use of urban space that can and should be replaced with more profitable development whenever the opportunity arises. In Seattle, framing that augments urban agriculture’s legitimacy as a source of livability amidst intensifying urban development overlooks the fact that rapidly appreciating neighborhoods become unlivable for residents at the bottom of the income distribution, who end up with greater food insecurity and likelihood of displacement regardless of garden permanence. Just as the different ways of framing urban agriculture’s benefit have been unequally strong as a claim for garden permanence, the different organizational configurations and environments in each city have been unequally conducive to social movement mobilization that could challenge elite interests and push city officials beyond their original willingness for garden preservation.

In terms of the organizational environment, evidence from Milwaukee and Seattle indicates that civic conventions conducive to bottom-up governance work to support the process of legitimizing urban agriculture, but it appears to have been the discursive opportunity structure of mistrusting elites, absent in Milwaukee but present in both Philadelphia and Seattle, that has facilitated mobilization in defense of threatened urban agricultural spaces. Different organizational configurations across the three case-cities are instructive for understanding the dynamics of organizational hybridization, especially from community-based to social movement activities. Across the three cases, I found only one example of a community-based organization effectively taking up the work of a social movement organization —the P-Patch nonprofit. Developed as a parallel organization to support the city’s P-Patch program by providing a forum for volunteer site leaders to share strategies for garden management, the P-Patch nonprofit gained legitimacy as a representative of gardener interests while maintaining an organizational structure independent from the city program that allowed for outsider social movement mobilization when needed. Both of these features facilitated the P-Patch nonprofit’s success in SMO activities, but these activities were organized on a temporary basis, and their framing reflected the relatively privileged perspectives of the nonprofit’s volunteer leaders. In contrast, Soil Generation has arisen in Philadelphia as a counterpoint to PHS, a CBO that did not prioritize gaining legitimacy from gardeners and has been perceived as coopted because of its close relationship with city leaders. Soil Generation has functioned as a SMO since its inception and has kept up its social movement activities for the long term. With leadership explicitly oriented to the needs of poor people of color, Soil Generation is advancing a frame that re-legitimizes urban agriculture as worthy of permanence, while also insisting on policy that will address the broader needs of the city’s low-income gardeners—especially their need for affordable housing. While not generalizable to all organizations in all cities, comparing the example of Soil Generation to the other organizations in this study suggests that organizations formed with a social movement orientation may simply be better positioned to advocate for policies that run counter to elite interests than organizations formed as community-based organizations to provide services.

In Milwaukee, none of the organizations involved in building, maintaining, or advocating for urban gardens can really be considered a social movement organization. The main community-based organization that manages gardens in the city, Milwaukee Urban Gardens and now Groundwork Milwaukee, has occasionally called for gardeners to write letters on behalf of a favorable policy, but the group has never organized to pressure city officials for garden preservation or other policies that go beyond what the city is interested in doing for its own interests. Similar to PHS in Philadelphia, Groundwork Milwaukee now draws a decent share of its funding from green space maintenance contracts with the city, establishing organizational commitments that would conflict with outsider strategies for social movement mobilization. Across the three case-cities, evidence suggests that the switch from CBO to SMO is challenging because CBOs often must seek resources and legitimacy from city officials, large funders, and other elites; over time, their work as service providers appears to build up connections and commitments to other organizations that can leave them coopted or less focused on the needs of more marginalized members, clients, and constituencies. Of course, this finding only reflects analysis of a small sample of organizations, and additional research with larger samples would be needed to confirm if this pattern is widespread, but it conforms with earlier findings about the process of organizational cooptation over time . While Groundwork Milwaukee provides one example of a CBO unlikely to take up confrontational politics, the Milwaukee Food Council is an organization more like Soil Generation that was formed to advance policy goals, cannabis indoor greenhouse and due to its relative independence from the local government this organization might be better positioned for outsider strategies of social movement mobilization. However, the Milwaukee Food Council mostly counts leaders from other organizations as its members and does not have much of a direct relationship with gardeners or the general public. In other words, unlike Soil Generation, the Milwaukee Food Council has not gained legitimacy as a representative of the city’s gardeners and marginalized residents. Even if the Milwaukee Food Council had legitimacy as a representative of gardeners and a large, active base of supporters to mobilize in the push for more permanent urban agricultural spaces, because of the benefits for which urban agriculture has been legitimized in Milwaukee, the city’s civic conventions, and the political-economic reality in which currently cultivated lots are seen as a potential development lifeline for reviving the city’s economy, this organization would still face a steep challenge in convincing city officials or the general public that permanent gardens are the best policy. Across all three cities, the legitimation activities of garden organizations and the policies they have achieved to increase longevity for the city’s gardens are reflected in the physical manifestations and geographical distribution of gardens. While there are certainly similarities between the community gardens in all three cities, the forms and ideas about urban agriculture that people are likely to encounter as they move through urban space are different. Among the three cities, the prevalent urban agricultural forms in Milwaukee can be understood as the most impermanent. In Milwaukee, one is more likely to observe large, mowed lots with only a few trees or garden beds that represent the legacy of MUG’s early attempts to function as a land trust, which backfired when these sites did not have enough support or interest from nearby residents to be maintained in full form. This particular form is certainly not widespread in Milwaukee, but it is virtually absent in the other case-cities and it serves to reinforce ideas about community gardens as temporary land uses. Another distinct feature of Milwaukee’s urban agriculture landscape is the prevalence of youth job training programs and food businesses that package and distribute items grown on urban farms. Someone moving through the city is as likely to encounter a site where young people work together to tend crops as they are to encounter a community garden with individual plots claimed and cared for by different people. Both of these urban agricultural forms can provide important nutritional and social benefits for people in need, but the employment and commerce-oriented nature of Milwaukee’s urban agriculture leaves open more possibility for relocating urban agriculture to make way for other kinds of development. In Philadelphia, there are numerous traditional community gardens—certainly more than in Seattle or Milwaukee—but their presence is dwarfed by the 13,000 vacant lots that are maintained with PHS’s signature clean-and-green treatment. As in Milwaukee, this form of urban agriculture signals impermanence, but unlike the spaces tended by Milwaukee’s youth these sites are not growing food—only trees and a few ornamental plants that can be easily kept up by the circulating maintenance crews. Someone moving through the city is more likely to encounter a clean-and-green lot than a community garden or farm, but many such spaces do exist. Some of these spaces announcing themselves with signs, murals, and tributes to groups who have ensured their existence, while others keep a low profile to avoid what gardeners perceive as the likelihood the city will sell the lot if they learn it has a garden. Regardless of their outward appearance, and despite not being the focus of the legitimizing narrative that PHS amplified for many years, hundreds of gardens in Philadelphia have provided food, a sense of community, and other benefits to residents in many neighborhoods. In Seattle, the most common form of urban agriculture is the P-Patch community garden, most of which have individually tended plots and common areas with space for the public to sit and enjoy urban nature. Someone moving through the city is likely to encounter a P-Patch with signage announcing the program and perhaps an upcoming community event to be held in the space. These elements reflect the strategic efforts that P-Patch advocates have made over the years to bolster the program’s legitimacy in the eyes of city officials and the non-gardening public, given that they have secured virtual permanence for the gardens as a land use, but must still work to maintain the spaces’ public legitimacy and funding. As we consider what form of urban agriculture someone might encounter as they move through each city, we should also consider who is likely to be having the encounter in the first place. Over time, as one part of the wider urban processes of economic competition and land use contestation, organization-led efforts to legitimize and secure urban agricultural spaces have not only influenced the form that these spaces take, but also where the gardens have survived and who is most likely to be occupying nearby urban space to begin with.

Plants will establish more readily in the shallow areas and less so where the water is deeper

As the US economy is shifting away from manufacturing, Florida argued, continued growth derives from an ascendant group of workers he called the “creative class”—people who work in science, technology, engineering, design, and other knowledge-based sectors . Because their work is intellectual rather than physical, these individuals are not as tied to particular locations, and they can choose to live in whichever cities they find attractive; in other words, particular types of use value can serve as a basis for increasing exchange value. The types of use value most important to the creative class include diversity, individual expression, and loose community with many weak social ties . Indeed, Florida highlighted Seattle as a creative city with all the ingredients to attract the creative class, and the P-Patches are exemplary of the urban character that Seattle was offering: they are filled with art and with all different kinds of people getting to know one another in loose communities . Without having the vocabulary of creative cities, P-Patch advocates in the 1990s framed the value of their gardens for city leaders in terms that align well with attracting the creative class. While the theory of creative cities appears to offer a resolution to the tension between use and exchange value in urban growth dynamics, in reality the tension is simply displaced. Urban growth entrepreneurs were quick to take up Florida’s ideas in their development strategies, and critics were equally quick to decry the downsides . Florida himself acknowledged that creative cities tend to have higher levels of inequality as the economy is increasingly bifurcated into a creative class and a service class . People in creative cities who do not belong to the creative class cannot fully enjoy the benefits of its use-value-rich amenities. The increasing exchange value in hip neighborhoods—or, in cities such as Seattle, hydroponic racks all neighborhoods—contributes to higher rents and displacement of lower income and marginalized people .

Furthermore, while creative cities make a show of celebrating racial and ethnic diversity, the reality is often a superficial multiculturalism lacking substantial engagement with institutional racism and the inequalities it produces . In creative cities, the downsides of increasing exchange value are borne even more heavily by marginalized people. This diminished use value is just easier for a creative city’s more affluent residents to ignore than the traditional downsides of growth such as noise, traffic and air pollution—pervasive downsides that P-Patches help ameliorate for Seattleites. For the last 30 years, the political economy of Seattle has enabled continued investment that has helped solidify the status of the city’s community gardens, but nothing about this political economy ensures that the gardens are providing the potential benefits most needed by the city’s marginalized residents—or even that they remain accessible to these communities at all. The city’s gardens do produce a lot of food, with some of it directly feeding low-income gardeners in the P-Patch program and other gardens such as the Danny Woo International District Community Garden, which serves primarily Asian-American residents of nearby affordable housing. As described in chapter 3, the City ensured that as its P-Patch program expanded, new gardens accessible to low-income residents were prioritized, and P-Patch gardeners also grow tons of fresh produce for the city’s food banks. Food bank donation is a longstanding tradition in the P-Patches, but it is not a requirement for participants and is contingent upon the available time and generosity of current gardeners. In good years, the total amount of produce donated by P-Patch gardeners exceeds 40,000 pounds; however, as Seattle has become increasingly unaffordable, the number of people relying on food banks has also increased. Even before the pandemic, food banks were distributing more than 22,885,000 pounds of food a year .

From 2007 to 2011, average monthly visits to food banks in Seattle doubled from 61,401 to 122,197 . The rate of food insecurity in Seattle grew from 7% in 2007 to 13% in 2019 . The fresh, organic produce that flows from P-Patches to low-income gardeners and other food-insecure Seattleites is not insignificant, but the rate of growth in food bank donations is not keeping up with the rate of growth in rents and attendant growth in food insecurity. Food provision is one of the key benefits that urban gardens can offer low-income residents, but others matter as well. Low-income neighborhoods tend to suffer from more blight, higher crime rates, and lower neighborhood social cohesion, and gardens have been extolled for their potential to improve low-income neighborhoods along these dimensions. However, if the neighborhoods become unaffordable for low-income people, then those residents have to move, and the neighborhoods’ improvements are moot for them. In Seattle, over the last two decades since garden advocates won preservation victories and significant resources to expand the P-Patch program, real estate values have also increased dramatically citywide . Staggering increases in median home values—up 93% from 2012 to 2018—have priced many people out of formerly affordable neighborhoods or out of the city entirely . As one outcome of this extreme housing market, the Laotian gardeners who helped build Bradner Gardens Park in the 1990s can no longer afford to live in the surrounding neighborhood. Overall, the program has evolved toward benefitting low-income residents because of its public mission and some of its partnerships, and P-Patch gardeners have long celebrated their racial and ethnic diversity although it does not seem that a lot of effort was put into cultivating leadership from minority communities. The social movement mobilized to prevent program cuts in the early 1990s and preserve threatened P-Patches thereafter was led by gardeners who were active in the P-Patch nonprofit.

Unlike the Pennsylvania Horticultural Society, this organization was seen as a legitimate representative of gardeners’ interests, but the movement the organization built was not framed around or led by gardeners of color, immigrants, or low-income people. This movement has been extremely effective at preserving community gardens and ensuring that public resources continue to support these spaces; today, P-Patch advocates quickly organize to protect any sites that become threatened . The P-Patch nonprofit has many of the features known to contribute to social movement success: skilled and experienced leadership, an engaged constituency, legitimacy in the eyes of decision-makers and the public, and sophisticated framing that resonates with their target audiences. However, this movement has been organized narrowly around preserving the P-Patches—resisting one of the symptoms of unrestrained urban growth, rather than challenging the logic of growth overall or any of its other ill effects. As Seattle real estate values continue to balloon, microgreen grow rack displacement continues apace, and the framing for preserving P-Patches does not address the detrimental impacts of growth on poor gardeners, residents of color, or others vulnerable to the ongoing displacement.Evidence from the three case-cities indicates that a local government’s ability to support urban agriculture is tied to its economic and fiscal situation. Of course, the status of the locality within the ongoing global competition to attract growth matters for the amount of resources available to invest in urban gardens. Since cities are continuously engaged in this competition, their status is always subject to change, and potential change in the city’s economic fortunes remains a top concern of elected officials and growth elites regardless of the city’s current success or failure in attracting urban growth. The history of all three cities shows that framing urban agriculture as a valuable tool to improve or insure a city’s economic standing has been an effective strategy for winning favorable policy and public investment. The appeal to growth interests has taken on different forms in the different economic and political contexts of each city, and in all three cases these economic rationales have consequences for the city’s gardens and/or for its marginalized residents. In Seattle, as the city was beginning to experience urban growth due to its strong technology sector, P-Patch advocates refined their efforts to legitimize community gardens by framing them as a neighborhood amenity that ameliorates some ill effects of urban growth, building a case to value gardened land alongside housing and commercial development and furthering the commodification of nature as a selling point for the city’s livability. This refined framing presents urban agriculture as a palliative for the alienation from nature and fellow humans that often occurs with urbanization . However, it does not address other social impacts of rising property values—particularly the affordability crisis that displaces the city’s low-income residents.

In Philadelphia, where economic downturn and disinvestment left 40,000 lots across the city vacant, PHS and other growth coalition members successfully argued that this land was a liability for the city, and that repurposing it for greening would help revitalize blighted neighborhoods and attract new capital investment. They were right; Philadelphia has turned its fortunes around and is now experiencing renewed urban growth, including rapidly increasing land values and gentrification in some of the city’s neighborhoods. With the floodgates opening to capital flows, gardens are getting swept away. Now, Soil Generation and its allies are trying to push back on the commodification of nature as a symbol of investment readiness that can flip vacant land from liability to asset, shifting the focus to the community members who have stewarded these spaces and arguing that they deserve to retain them—an outcome that would necessitate both the gardens and the gardeners being able to stay in place. In order for this to occur, the city’s Land Bank must implement its directives in a way that prioritizes community land uses in gentrifying neighborhoods, an uphill battle given the immense amounts of capital held by growth entrepreneurs vying for ownership of these spaces. Soil Generation’s ongoing organizing and framing around community control works to put power behind this struggle, and they have accomplished some early victories in framing the Land Bank’s mandate and revising the disposition process; however, it remains to be seen what the movement will ultimately achieve in terms of garden preservation and affordable housing. In Milwaukee, the city is still struggling to win greater capital investment and urban growth, and land is seen as a lifeline for this effort. Urban nature in the form of gardens and farms has been commodified as a tool for training and employing residents, a potential pathway to economic development that can ameliorate some of the worst impacts of capital flight that the city has experienced. Despite its poor fiscal situation, the cash-strapped city government still shows willingness to devote some resources and recruit public and private partners to invest in urban agricultural spaces. However, like PHS’s framing in Philadelphia, Milwaukee’s commodification of urban nature as training ground and space of economic production leaves open the ongoing possibility of replacing gardens and farms with any more profitable use that might come along.The preceding chapters have revealed how the main community garden programs and proponents in each city highlighted some of urban agriculture’s potential benefits over others, influencing the priorities for how community gardens were developed and managed over time. In addition to assessing what benefits community gardens are providing to surrounding neighborhoods, we can better understand their impact on a city by investigating where community gardens are located, and thus to which neighborhoods their benefits are accruing. As noted throughout this study, many of the benefits for which community gardens are celebrated are particularly important for low-income communities and marginalized racial and ethnic groups. The free or low-cost fresh produce these spaces can yield will matter most for food-insecure households, often associated with high-poverty neighborhoods and those with a higher proportion of Black and/or Latino residents . Urban blight, crime, and inadequate greenspace are also more common in neighborhoods with these characteristics, so the value of community gardens as safe, attractive, and healthy greenspace is also especially salient in such areas . Community gardens can support important cultural practices as well, since ways of growing food and medicine are meaningful traditions for virtually every culture. In this regard, the ability for immigrants to access community gardens is another key consideration for understanding whether urban agriculture’s touted benefits are available to those who need them most.As with any alternative food initiative, there is no guarantee that the benefits of urban agriculture will accrue to those who are most in need.

The wetlands under study differed widely in their capacity to remove contaminants from water

As chapter 5 will detail, longitudinal spatial analysis demonstrates that between 1980 and 2019, P-Patch gardens have become more accessible overall; moreover high-poverty neighborhoods in Seattle were originally further from the PPatch gardens than their lower-poverty counterparts, but that relationship has flipped over time such that proximity is greater for low-income communities today. Finally, in response to the “persistent skepticism” about gardens as a private use of public land, as Frank Kirk’s quote above describes, the P-Patch program leaders systematically incorporated public spaces into design and redesign plans for the gardens. In my analysis of PPatch Post newsletters, the code design for community was only applied twice for issues in the 1980s, but this code came up more and more often in the 1990s and especially from 1998 onward. New gardens were built with public features like benches or picnic tables, and such elements were added to the older gardens as they were renovated—especially when those gardens won Neighborhood Matching Funds to improve their spaces. Because of the civic infrastructure in Seattle, including numerous channels of communication between garden advocates, city officials, and other residents as well as the availability of public resources for garden development, P-Patch gardeners were able to maintain legitimacy for their organization and for the use of public land for urban agriculture by aligning their activities with widely shared values and public priorities. The priorities of city officials and P-Patch gardeners were not always perfectly aligned, however. As mentioned earlier, two P-Patches on public land were threatened by development in the mid-1990s. Gardeners first pressed their interests through the city’s bottom-up governance infrastructure, but it soon became clear they would not prevail through insider strategies .

While Seattle’s political opportunity structure is usually amenable to resident interests, indoor plant table competing resident demands—and the growth machine’s drive for increasing exchange value—exerted strong countervailing influence in these cases. When insider strategies proved futile, the garden advocates pursued outsider strategies to organize public opposition to the development plans. In this period of mobilization, Seattle’s civic conventions promoting distrust of elite control and expecting neighborhood involvement in decision-making helped form a discursive opportunity structure for framing the threat of garden loss in a way that would resonate well beyond the gardens. When the gardeners at the Mount Baker P-Patch learned that their city-owned lot was slated to be auctioned off for housing development, they joined with the local neighborhood association to come up with an alternative plan. With a grant from the Neighborhood Matching Fund, they conducted design charettes with P-Patchers, representatives of greening organizations, and other residents. The final product, a plan for Bradner Gardens Park, interwove P-Patches with numerous other uses such as a basketball court, family picnic area, public art, and native planting demonstration gardens. Advocates for Bradner Gardens Park presented the plan to city officials, and also visited the meetings of neighborhood associations from across the city to gain their endorsements. They built up legitimacy for the Park proposal through the civic infrastructure of Neighborhood Matching Funds and neighborhood association endorsements, and they framed their proposal as the epitome of neighborhood planning, civic participation and collaboration among diverse groups in a community. City leaders remained steadfast in their desire to see housing on the site, however, so the garden advocates devised an initiative to prevent the auction. With the help of a former were reneging on their previous deal.The argument that the city could not be trusted helped to whip up opposition to the proposed move, and it also resonated with one council member who reportedly didn’t care about PPatches but was moved by the argument about fairness.

Ultimately, council members did approve a plan to move the garden, but only on the condition that gardeners were given a better replacement site and logistical and material support for the move. These concessions made a big difference for the future direction of the Interbay P-Patch, which is a thriving community garden and destination for neighborhood residents today. In both of these cases, Seattle’s garden advocates were able to strike a nerve for the general public by framing the city’s plans as a betrayal of the self-government expectations they held, and by arguing that these moves needed to be resisted in order to hold the government accountable to its citizens. Ultimately, in both cases, council members originally opposed to the gardeners’ requests ended up voting in their favor. The garden advocates’ leveraging of Seattle’s civic conventions is evident in analysis of documents and interviews from the three cities, which shows that the code for fairness or justice was more than twice as common in Seattle as in Philadelphia and more than three times as common as in Milwaukee. The bulk of these codes applied to documents in the period of October 1995 to September 1996, when the Bradner and Interbay resistance efforts were broadening from insider strategies into outsider strategies involving criticism of the city and mobilization of both gardeners and the public. These examples demonstrate how social movement mobilization was effective in Seattle and accomplished long-term preservation for many of the city’s gardens. However, the movement mobilized in the mid-1990s was framed around a symptom—development threats to specific gardens—and not around the underlying economic dynamics driving garden displacement. While all of the gardens on public land are effectively permanent thanks to movement organizers’ victory in passing Initiative 42, gentrification has continued apace in Seattle; among the many low-income residents who have been displaced due to rising housing costs are gardeners who can no longer afford to live near the sites they helped to build, including Bradner Gardens Park.

In this way, the local garden preservation movement in Seattle draws a clear contrast with that in Philadelphia, where garden loss is framed in connection to the broader context of structural racism, neighborhood disinvestment, and growth machine logic that threatens vulnerable people as well as vulnerable spaces.This chapter has demonstrated multiple ways that civic conventions structure opportunities for garden organizations to legitimize their activities and to build broader support for urban agriculture as a land use. Civic institutions that support public input into policy making have served as political opportunity structures to legitimize urban agriculture as a land use desired by residents. Civic ideas can serve as a discursive opportunity structure that garden advocates can leverage to frame the need for social movement mobilization in support of threatened gardens, whether or not these spaces have gained legitimacy through formal policy channels. When a city’s civic conventions include infrastructure for bottom-up governance, this infrastructure provides a political opportunity structure for supporting resident demands such as space for community gardens and urban agriculture. A participatory governing process can rationalize garden development in accordance with broader public interests and concerns while building legitimacy for community gardens as a land use. Garden organizations in Seattle and Milwaukee have bolstered the legitimacy of their gardens by taking advantage of civic conventions conducive to bottom-up governance, including the civic infrastructure of grant programs for resident-initiated projects and accepted channels for communicating resident desires. In these cities, conventions for bottom-up governance provided a political opportunity structure to legitimate urban agriculture as an activity in line with broader public priorities. The same political opportunity structure did not exist in Philadelphia, where PHS established legitimacy for its Philadelphia Green program as a provider of city beautification services and a catalyst for neighborhood reinvestment, with less emphasis on the importance of fulfilling resident desires for growing space. Instead, gardens in Philadelphia struggled to gain legitimacy in the eyes of city officials even as many such spaces were used and valued by neighborhood residents. The city’s redevelopment efforts led to the loss of numerous gardens in the early 2000s, plant growing stand when city officials treated these spaces as “vacant” rather than land already under legitimate use. With PHS having legitimized its garden support activities as temporary measures to promote redevelopment, the organization was not positioned to challenge the loss of gardens through that very redevelopment. Indeed, due to PHS’s dependence on city contracts for greening services and its association with the city’s social elite, the organization stood to lose legitimacy in any open confrontation with public officials. When PHS did not mobilize its gardeners to challenge the city’s land use policies, another organization did. Situating the pattern of garden removal in the historical context of dispossession and racial injustice, Soil Generation has mobilized the city’s growers to push for greater community control over land use decisions. Their framing legitimizes urban agriculture as stewardship of land long abandoned by property owners and the city, while delegitimizing the city’s development plans by drawing on widely shared cynicism about the government’s effectiveness and trustworthiness. This cynicism has provided a discursive opportunity structure in which calls for ongoing mobilization to ensure government accountability have resonated widely. In coalition with other groups organizing for economic and racial justice, Soil Generation has gained legitimacy as a genuine representative of the interests of gardeners and communities of color in the city, and the organization has sustained outsider strategies to pressure the city government to transfer land ownership to community gardeners. Discursive opportunity structures in Seattle have similarly supported social movement mobilization when gardeners sought to preserve gardens and public officials resisted.

Widely shared ideas about the value of civic participation underlay the development of the P-Patch nonprofit, its successful cultivation of volunteer labor over decades, and its mobilization to stave off garden loss and achieve permanence for the P-Patches. As in Philadelphia, civic ideas about distrust of elites contributed powerfully to the local discursive opportunity structure; by framing Initiative 42 as a necessary bulwark against city officials selling off parks, P-Patch advocates won broad public support for their proposal and secured stronger protections for the city’s gardens than public officials were initially wiling to enact. In contrast, Milwaukee’s civic conventions hold more of an assumption of good governance on the part of city officials, and the local discursive opportunity structure has been far less favorable to social movement mobilization. Garden advocates have achieved longer term land access and more permissive zoning policy through insider political strategies, but many gardens in Milwaukee remain classified as a temporary use. Without an organized effort to engage gardeners in civic action, and without a widely held belief in the need to mobilize to hold the government accountable, the city’s garden advocates seem to lack the tools to pressure city officials into making more gardens permanent. In terms of urban political economy, insider strategies that frame urban agriculture’s value to align with the city government’s priorities will likely reflect growth machine logic. Land use policies that elected officials will happily pass, such as small matching grants to support green space improvements and ordinances allowing beekeeping, tend to increase use value for residents in ways that do not undermine the potential for increasing exchange value as well. To achieve permanent tenure for community gardens and urban farms means removing the land from the city’s development portfolio—something that runs counter to the standard motivations of most city officials. The cases of Philadelphia and Seattle demonstrate how garden advocacy organizations have mobilized social movement activity by drawing on discursive opportunity structures to successfully frame the need for garden permanence as part of a wider struggle to preserve resident interests in the face of potential political corruption. Alongside these more confrontational efforts, organizations in both Philadelphia and Seattle also used insider strategies for some of their efforts to legitimize gardens. As Chapter 4 will explain, growth machine logic endures as a powerful force in shaping the framing, policy, and practice of urban agriculture in all three cities.A city’s civic conventions form an important piece of the organizational environment in which community gardening programs develop and define themselves. Yet what is possible for urban agriculture in any given city is also contingent upon its political-economic context. As urban political ecologists would describe it, ideas about appropriate uses for urban space combine with material flows and conditions, as well as ideas governing the legitimacy of governments themselves, in order to determine the actual production of urban socio-nature . In this regard, the distribution and character of urban agriculture in any city is influenced by local economic pressures, the sources and extent of public resources, and political factors at larger scales such as the laws and activities of state and federal governments.

Sedimentation is one of the primary pathogen removal mechanisms active in wetlands

This is not to say that active racial bias was applied in hiring and appointing leaders, rather that low or no pay likely served as a barrier for gardeners of color in light of persistent racial wealth gaps . The demographics of P-Patch leadership are likely to have impacted the perspective from which both organizations operated, especially the framing of the benefits of community gardens. P-Patch volunteers assiduously grew and measured their food bank donations, which have no doubt been helpful for food insecure Seattleites; however, produce distributed through food banks treats the symptom of hunger, rather than its root cause of economic inequality . Likewise, community building is an important and hard to measure process for maintaining healthy social dynamics in cities . However, when community-building is framed as a relief-valve for the pressures of increasing urbanization, as was often the case in the P-Patch’s framing, it reflects an ideological orientation that seeks to accommodate rather than challenge ideas of inexorable urban growth. Moreover, as Chapter 4 will explain, the sense of community engendered in the P-Patches is part of what has made Seattle such an attractive city for the “creative class” whose purchasing power and population growth have contributed to gentrification in most Seattle neighborhoods. Among the three case-cities, Seattle’s urban agriculture movement, in particular the organized efforts to preserve P-Patches in the 1990s, grow table has been the most effective so far at accomplishing policy change that secures community gardens as a permanent land use.

The organizational structure, pairing a city-run garden program with a nonprofit fundraising and advocacy organization, appears to have worked very well to develop and manage gardens in a stable way while facilitating gardener mobilization when needed and encouraging leadership from gardeners themselves. However, gardeners from more marginalized communities have almost never taken on these leadership roles, and the mobilization to protect gardens has not addressed the question of securing low-income gardeners themselves in the neighborhoods they have helped to shape.Urban agriculture has gained legitimacy as a land use in each of the three case-cities, and the benefits with which it is most associated reflect the strategies that the main gardening organizations in each city have pursued to gain and maintain their own legitimacy. In Milwaukee, MUG attempted to gain legitimacy as a land trust, but when they were unsuccessful in convincing a sufficient donor audience that garden preservation was a meaningful cause, MUG ultimately joined Growing Power, Walnut Way and the Victory Gardens Initiative in focusing on youth engagement and employment. Together, these organizations built legitimacy for urban agriculture as a tool for job training and economic development. Philadelphia Green gained legitimacy as a program of the well-established Pennsylvania Horticultural Society, and in working to maintain its legitimacy for the organization’s donor base, program leaders demonstrated how greening can work as a tool for blight removal and neighborhood revitalization. However, PHS and Philadelphia Green did not invest as much effort in building the program’s legitimacy with urban gardeners themselves, and another group has claimed that role. When community organizers were mobilizing to change city policy and defend threatened gardens, they ultimately removed PHS from their coalition and formed Soil Generation, an organization staking its legitimacy on its representation of Black and Brown growers in the city.

Rejecting the notion that urban agriculture is a legitimate land use mainly for its revitalization potential, Soil Generation is framing the need for preservation as an issue of community control over land use decisions. The City of Seattle’s original funding for the P-Patch Program was legitimized as support for recreation, but the program and its advocates have maintained legitimacy over time by documenting and emphasizing other benefits more in keeping with the departments in which the program has been housed—that is, providing food for people in need and a network of community-building spaces that bring diverse people together . While urban agriculture has been legitimized as a land use in all three case-cities, the different framings do not all translate equally well into making claims about the need to preserve community gardens in the face of more profitable potential land uses. For example, the economic development potential of urban agriculture confers legitimacy on such spaces, but does not preclude replacement with another form of development that would likely yield more jobs. Legitimacy is built up over time; once urban agriculture has come to be associated with particular benefits in a given locale, shifting the narrative proves more difficult. Furthermore, shifting an organization’s emphasis becomes more difficult once that organization has gained legitimacy and built up ties and commitments with other organizations in its environment. The challenge of gaining legitimacy to begin with was more difficult for MUG than for Philadelphia Green or the P-Patch program and its supporting nonprofit, because MUG lacked any affiliation with an existing, already-legitimized organization. When seeking policy change to increase land tenure for gardens, MUG, Philadelphia Green and the P-Patch Program all erred on the side of insider advocacy, having built close relationships with city agencies . When insider strategies were not enough, the P-Patch nonprofit had relatively more flexibility than these groups to parlay its organizational legitimacy into social movement organizing.

Since the PPatch nonprofit had gained legitimacy as a forum for supporting gardeners, rather than as a garden site administrator, its primary legitimizing audience was the gardeners themselves, and the organization depended relatively less on approval from city officials. Framing appeals for collective action as looking out for the interests of its primary, already-engaged audience, the PPatch nonprofit was able to take up the function of a social movement organization with relative ease . Finally, let us consider how the various organizations’ efforts to legitimize their operations have impacted the physical institutionalization of urban agriculture within each city’s landscape. For example, most Milwaukee residents and visitors are just as likely to encounter young people selling cottage goods made from produce they grew as they would be to encounter the space in which the products were grown. Given the relative scope of Philadelphia Green’s different projects, residents and visitors in Philadelphia are far more likely to see lots with the “clean-and-green” treatment than they are to see community gardens. Meanwhile in Seattle, the P-Patch gardens have been gradually developed into public gathering spaces rather than just growing spaces, and residents and visitors are increasingly likely to encounter them as inviting, park-like places. Thus, the organizations have legitimized urban agriculture around some benefits rather than others, not only discursively through media coverage, publicity, vertical rack and political engagement, but also materially through the manifestations of their work that reinforce particular ideas about urban nature.Organizational sociologists and social movement scholars have long emphasized the influence of external factors on organizational practices and outcomes . Yet an aspect of the organizational environment that has not received much attention in the literature is the locality’s civic conventions . Civic conventions are shared beliefs about expected and acceptable forms of interaction between the government and the polity, an institutionalized understanding of “how we do things around here” . This chapter will demonstrate how civic conventions are especially influential for hybrid organizations as they attempt to expand into a new organizational function which positions them differently with respect to civic action. Deploying the concept of civic conventions, I contribute to the literature on hybrid organizational forms by exploring the dynamics at work when service providers take on social movement work, rather than the reverse scenario described by Minkoff . Unlike hybrid organizations that begin as movement organizations and later take up service provision as a form of civic action, urban agriculture groups initially work to organize communities in the civic action of transforming land and must then take up social movement work later, when the transformed land becomes threatened. In doing so, garden organizations must navigate idiosyncratic local expectations regarding civic and political engagement. Organizations that build their legitimacy around social movement activities may be able to push the boundaries of local civic conventions, but organizations that are legitimized for community service provision face an extra challenge in gaining legitimacy for new activities, and thus pressure to conform to extant civic conventions is stronger. Building connections between organizational theory and the literature on social movements, I argue that the local civic conventions can be understood as a combination of political and discursive opportunity structures, working together to shape the terrain on which hybrid organizations cultivate civic participation of various forms among some or all of their members.

When they first form, urban garden organizations must work to establish legitimacy for themselves as community-based service providers. In order for a garden organization to be viewed as legitimate, the gardeners must be seen as contributing to the public good rather than as benefitting unfairly from public resources such as land and water. Even when urban garden organizations become familiar and widely accepted in a city, the use of urban land for agriculture is almost always viewed as a temporary practice . Once gardens are established, they often become quite meaningful to the gardeners and those living nearby; this emotional connection makes the loss or removal of the garden a difficult prospect. Facing an impending removal or changing economic conditions that increase gardens’ vulnerability to development, garden organizations must work to build a new kind of legitimacy for urban agriculture as a permanent fixture of the urban landscape. This effort requires new framing processes and political strategies, often including social movement mobilization. The strategies that can be pursued at this point will depend somewhat on the local civic conventions, as well as the existing frames that have been used to legitimize garden organizations.In this chapter, I highlight the role of civic conventions throughout the life of urban garden organizations and the movements they spur to preserve urban agriculture as a land use. When urban garden programs are building their initial legitimacy, when gardens are about to be replaced with a different land use, or when garden advocates propose a change in local policy that would increase the long-term security of growing spaces, they can build strategies that draw on local civic conventions to amass broader support from the general public . I discuss two main ways that civic conventions can promote garden legitimation at these different points in time. First, civic conventions conducive to bottom-up governance can help build the legitimacy of urban agriculture as garden organizations are getting started and seeking out basic resources and support—in other words, as the garden programs are seeking to gain legitimacy as community-based organizations. Like other resident activities and use-value rich land uses, urban agriculture tends to have its strongest base of support at the grassroots level. If the municipal government is generally receptive to resident preferences and interests, this convention creates a relatively easy way for resident demands for urban agriculture to be incorporated rationally into local policy. In Seattle and, to a lesser extent, in Milwaukee, civic conventions which held that city officials should be receptive to bottom-up governance created many opportunities for residents to express their desire to use vacant land for growing food directly to key decision-makers, and the cities’ main garden organizations and policies gained legitimacy through this process. Conversely, in the case of Philadelphia, civic conventions carry far less expectation for bottom-up governance. In this city, cynicism about government runs high in part because of a complex, opaque bureaucracy that seems to discourage formal resident input. In this case, when cultivated lots were being sold without gardeners’ prior knowledge or input, lack of access to decision-making and perceived injustice became rallying cries for broader mobilization around community control of land and urban planning. Comparing the social movement dynamics in Milwaukee, Philadelphia, and Seattle, in this chapter I show how civic conventions present a landscape of discursive and political opportunity structures that hinder or make possible certain strategies for achieving an organization’s desired policy outcomes. Civic conventions that exist as widely shared ideas about what is unacceptable for, expected in, or salient to the local policy making process can be considered an aspect of the local discursive opportunity structure. That is, these conventions are cultural understandings of what is reasonable and legitimate in the context of local policy making .

This work also addresses Scanlan’s call for sociologists to engage more with the issue of food security

More research is needed to investigate the dynamics involved when organizations blur the lines between community-based and social movement work. When they have gotten involved in land use contestation, organizations that coordinate and advocate for urban agriculture illustrate a variety of strategies by which community-based organizations can work to assert resident interests and achieve political victories for less powerful groups. Urban agriculture reflects the on-the-ground blurriness between community based organizations and social movement organizations, as the groups that practice and advocate for community gardening and urban farming take many forms. A range of organizations exists to direct activity at a single garden or farm, to oversee citywide networks of community gardens, and/or to advocate for the political interests of urban agriculture practitioners—particularly legal status and land access. This dissertation provides a comparative historical analysis of urban agriculture organizations in three US cities, focusing on their efforts to secure land for gardens by promoting various benefits of urban agriculture and organizing pushes for municipal policy change, and providing insights about the dynamics of urban political contestation and the nature of hybrid organizational forms that work at the boundary between CBOs and SMOs. Situated in the context of multifaceted environmental degradation, state retrenchment, market primacy, and widening inequality, indoor grow shelves the urban agriculture organizations described herein provide insight into emerging possibilities for counter-hegemonic action at the local scale.

Gaining permanent access to urban land for the purpose of social reproduction through agricultural initiatives means asking municipal governments to cede some control of one of the few domains from which they haven’t willingly rolled back in the last 50 years: land use governance. In this way, it is similar to other prominent citizen efforts today like the growing calls for community policing. Efforts to legitimize community gardens as a long-term land use are also indicative of wider struggles to redefine the value and place of nature in schema that determine collective decision-making. By examining the ways in which urban agriculture organizations navigate an environment with limited resources, public skepticism, often underprivileged and politically inexperienced members, and powerful countervailing political interests, we can better understand the dynamics required to accomplish meaningful structural change in modern cities.Organizational scholars have long investigated how an organization’s features, including its goals, structure, and relationships with other organizations, influence its lifespan and the outcomes it achieves. This chapter will build upon existing research about third-sector organizations , which has shown how decisions made in the context of these features matter for the success of civil society organizations. Day-to-day decisions about the actions an organization will take—strategies to pursue resources, the narrative communicated to target audiences, the nature of events and services, and the people they will be targeted to—are central to how the organization navigates its environment and what it accomplishes. In all three cities, such decisions made within urban agriculture organizations served to legitimize the organizations themselves; moreover, as organizational actors worked to demonstrate that their spaces could achieve outcomes desired for the organization’s own legitimacy, they prioritized some of urban agriculture’s potential benefits over others. In so doing, these organizations ultimately shaped the local narratives about what urban agriculture could offer each city.

This chapter contributes new perspective on the ways that an organization’s strategic pursuit of legitimacy not only works to institutionalize the organization itself, but may also work to institutionalize ideas and social forms in the physical as well as the organizational environment. I argue that organizational sociology can further extend the concept of institutionalization by drawing on urban political ecology’s insights regarding the interplay of discursive and biophysical processes in reshaping urban space and, by extension, reshaping public understandings of socio-environmental space and the organizations that manage it. The three cases demonstrate different ways that the value of urban growing spaces can be socially constructed through organizational activities and discourse. Garden organizations legitimize urban agriculture to legitimize themselves, and their strategic decisions to attract the resources they need for survival have a broader impact on the path along which urban agriculture develops—both spatially and socially—in the city. The current chapter will trace the different ways in which gardening organizations in Milwaukee, Philadelphia, and Seattle have established and maintained legitimacy for themselves and for the unconventional land use of urban agriculture, both building appreciation for community gardens and sustaining the requisite labor to maintain these spaces for long periods of time. For one thing, sustaining labor requires systematizing the operation of urban gardens and farms, many of which are started ad-hoc by small groups of residents whose efforts may be episodic. Building legitimacy for urban growing spaces rests in part upon presenting consistently well-maintained sites, so that non-gardening residents are more likely to see the sites as a benefit than they are to resent them as a nuisance. The potential for growing spaces to be seen as legitimate only if their appearance conforms to prevailing ideas of appropriate urban nature reflects a wider dynamic that urban political ecologists have noted, wherein the same physical elements can be seen as either assets or liabilities depending on their arrangement, location, and cultural context .

Beyond the aesthetics, urban gardens and farms are more likely to be seen as legitimate land uses if claims about their benefits are supported with evidence. In all of the case-cities discussed here, garden organizations systematically gathered evidence over time that showed urban agriculture sites providing certain benefits for nearby residents and the city at-large. The major community gardening organizations in Seattle, Philadelphia, and Milwaukee have developed systematic processes to manage labor and to maintain a narrative about the value of their organizations’ work. In each city, organization leaders framed the value of urban agriculture around particular benefits and then supported this narrative through organizational decisions and data collection. In Milwaukee, urban agriculture’s employment potential was foregrounded, while in Philadelphia the role of greening in neighborhood development was emphasized, and in Seattle garden advocates built a narrative around the food production and community-building benefits of urban agriculture. Importantly, given that urban agriculture cannot provide all of its potential benefits simultaneously, the choices made by organizational leaders in pursuit of some benefits meant less emphasis was placed on others. Over time, as these organizations amplified the narratives that maximized their own resource acquisition and legitimacy, local perceptions of urban agriculture and its physical manifestation across the city were increasingly shaped by the organizations’ touted benefits. With these benefits reinforced in the minds of political leaders and the general public, and less attention given to other potential benefits, in every case urban agriculture has institutionalized discursively and materially toward certain benefits over others. In all three of these cases, the legitimacy of urban agriculture was bolstered by some degree of support from officials in the local government; however, city officials are also broadly committed to the logic of urban growth and increasing exchange value, especially those who have power over land use decisions. At junctures when development pressure threatens the use of urban land for agriculture, indoor garden table a narrative legitimizing gardens around particular benefits is rarely enough to solidify their value as the highest and best use of developable land. In the face of such challenges, social movement mobilization becomes essential. Social movement activity requires significant time and resources, and the main garden organizations in Milwaukee, Philadelphia, and Seattle have not sustained social movement activities for the long-term to the same extent that they have invested in the systematic processes that legitimize their organizational activities. Nevertheless, at critical junctures when gardens have been threatened, each of these organizations has confronted the need for movement-building, or movement-like activities, in order to secure threatened land. In these instances, an organization’s existing commitments, its legitimacy, and the particular narrative used to legitimize urban agriculture often constrain organizational options in pushing for preservation. As this chapter will demonstrate, decisions made by the leaders of large garden organizations have an outsized influence on the public narrative legitimizing urban agriculture in their city. Critically, if organizational leadership is not developed from within the communities most in need , then the local urban agriculture system is unlikely to be tailored to their interests, because the needs of the urban growth machine—which are at odds with the needs of the poor—will impose themselves without fail on any question of urban land use.

Existing research shows that local food initiatives and other interventions to make cities more “sustainable” are still likely to manifest as uneven development that further privileges some neighborhoods and groups over others . While many of the potential benefits of urban agriculture are promising vehicles to alleviate symptoms of inequality, such an outcome is not automatic; instead, benefits sometimes accrue to more privileged groups while further disadvantaging those at the margins . Furthermore, organizational leaders may be more focused on treating the symptoms of injustice, rather than changing the underlying structural causes, if they do not have lived experiences of inequality and marginalization . Even if movements and organizations do pursue structural policy change, they may still reproduce unequal power dynamics in day-to-day practices and interactions . Thus, the extent to which organizational leadership comes from poor urban residents, people of color, immigrants, and other marginalized groups will impact the organization’s outcomes through both the movement strategies pursued and the organization’s everyday activities. The following sections will show how organizational decisions have been key to the successful legitimation of urban agriculture in Milwaukee, Philadelphia, and Seattle, while noting that the issue of developing leadership from within marginalized communities is still being worked out within urban agriculture organizations, just as within the broader alternative food and environmental movements. The chapter will highlight how organizational goals and decision-making affect the local narrative constructed regarding the benefits of urban agriculture and, ultimately, its role in the urban milieu. In so doing, this chapter strengthens the connections between urban political ecology and sociological theories regarding legitimacy, institutionalization, and social movements, by analyzing how community-based organizations’ pursuit of legitimacy over time reflects their relationships with the organizational environment and extends narratives of legitimacy into that environment, as well as the physical environment, which then shape possibilities for social movement framing and mobilization.As in many other US cities, interest in urban agriculture and growing food increased in Milwaukee amidst the economic downturn of the 1970s. Residents cultivated vacant land in Milwaukee through the Shoots n Roots program, established by the city in the early 1970s and taken over by the Milwaukee County University Extension from 1978 onward, as well as through more loosely organized activities on lots across the city. When a community garden in the rapidly appreciating Riverwest neighborhood was lost to development in the late 1990s, the displaced gardeners decided to form an organization to protect other sites like theirs. This is how Milwaukee Urban Gardens originated. MUG first formed as a land trust to purchase and preserve community gardens. In its early years, the organization was largely funded by a local benefactor who made a substantial anonymous donation that covered office expenses and one staff person’s salary for about 5 years. During this time, the organization’s goal was to build a name for itself, draw attention to the need to preserve local urban gardens from development threats, secure funding from additional sources, and purchase land for gardens—in other words, to gain legitimacy and attract the resources to sustain itself. However, without a robust donor base or relationships with large grant making foundations, the organization struggled to raise the additional money needed for land purchases. Operating on such a small budget, MUG was only able to preserve land opportunistically rather than based on the biggest threats facing existing gardens. Of the 5 sites that MUG eventually came to own, 3 of them were donated and only 2 were existing gardens. MUG worked to find interested residents and build new community gardens on the donated sites, but these gardens tended not to last. In 2010 MUG convened a land use policy task force in partnership with the Milwaukee Food Council. MUG’s director at the time, Bruce Wiggins, was a retired urban planner with experience in Philadelphia and Kansas City who prioritized addressing the city’s policies towards urban agriculture as a way to improve prospects for garden preservation.

I gathered archival documents from the Pennsylvania Horticultural Society and the City of Philadelphia

To assess the organizational dynamics and decisions involved in securing land for urban agriculture in Philadelphia, and to enable comparisons with Milwaukee, I collected data from similar sources. I interviewed 20 key informants with firsthand knowledge of activities in the city’s main community garden organizations and those who were directly involved in advocating for or implementing city policy related to urban agriculture. I also built a historical database of relevant articles from the city’s two main daily newspapers, the Philadelphia Inquirer and the Philadelphia Daily News. Integrating these data for my analysis, I gained a detailed understanding of the historical process by which urban agriculture’s value as a land use has been constructed and contested in Philadelphia, and I developed a unique dataset of PHS-affiliated gardens in order to map their locations over time. Since 1973, the City of Seattle has managed a network of community gardens through its P-Patch program. Like Philadelphia and Milwaukee, in the early 1970s Seattle was struggling with high unemployment and inflation, and the P-Patch program was created as a way to make unused urban land available for food production. Unlike Milwaukee and Philadelphia, however, Seattle’s P-Patch program is administered by the city itself. For almost 50 years, gardeners have succeeded in convincing city officials to maintain the program’s funding through municipal budget cuts and to avoid selling garden sites when development pressure increased during periods of economic growth . Today, the city devotes many acres of its own land to the P-Patch gardens, drying rack cannabis including some lots that were purchased specifically for new P-Patches. The city program’s staff assign garden plots, organize events, and train the volunteer site leaders who maintain gardens.

Early in the history of the P-Patch program, volunteer site leaders organized a nonprofit to improve communication and pool their expertise. This nonprofit took on an advocacy role in the mid-1980s when Seattle saw a period of economic growth and gardens began to face development threats. The nonprofit reorganized as a land trust to take ownership of a saved garden, Pinehurst, which became the city’s first permanent community garden. The nonprofit continued to advocate for stronger protections for the P-Patches, winning their inclusion in the city’s 1994 Comprehensive Plan, and passage of the Protect Our Parks initiative in 1997, which makes community gardens and other recreational spaces on city land virtually permanent. This policy ensured that the city could not sell any land used for P-Patches as the local economy has grown, fueled by its strong technology sector, even through a feverish real estate market in the mid-2010s. Today, the P-Patch nonprofit continues advocating for the gardens and providing administrative support to the P-Patches , while expanding out from Seattle to help promote community gardening across the region. To compare the movement strategies, organizational dynamics and decisions involved in securing land for urban agriculture in Philadelphia, Milwaukee, and Seattle, I collected data from similar sources in all three cities. For Seattle, I interviewed 17 key informants with firsthand knowledge of activities in the city’s main community garden organizations and those who were directly involved in advocating for or implementing city policy related to urban agriculture. I gathered archival documents from the P-Patch program office and the City of Seattle Municipal Archives. I also built a historical database of relevant articles from the city’s two main daily newspapers, the Seattle Times and the Seattle Post-Intelligencer.

Integrating these data for my analysis, I traced the historical process by which urban agriculture gained recognition and security as a land use in Seattle, and I developed a historical dataset of P-Patch gardens in order to map their locations over time. In chapter 1, I survey prior research on urban agriculture and relevant theoretical frameworks, including food justice, political ecology, urban political economy, communitybased organizations under neoliberalism, organizational legitimacy, and social movement processes. Situating my work at the intersection of these literatures, I highlight the limited attention paid to land use contestation for urban agriculture, on the one hand, and the broader need for more understanding of how community-based organizations contribute to urban social movements on the other. In the context of intertwined, increasingly urgent social and environmental problems, I argue that knowledge of how community groups secure long-term use of urban land for gardens has practical as well as theoretical significance. My research underscores the political and economic constraints that community-based organizations face and the potential pitfalls of framing the value of urban agriculture in various economic terms. In chapter 2, my analysis begins with an examination of the role that organizational structure and decisions have played in determining the trajectories of urban agriculture in each city. Once their garden programs were initiated, the main urban agriculture organizations in each city sought legitimacy for their activities as a requisite for maintaining their funding and land-use permissions. I show that in pursuit of legitimacy for their specific programs, these organizations also had to build legitimacy for urban agriculture more broadly; that is, they had to justify the unexpected presence of gardens and farms on urban land. As they interacted with funders, city officials and the media in pursuit of necessary resources, leading garden advocates in each city learned what these gatekeepers were most concerned about and framed their work accordingly. Selecting from among the many potential benefits of urban agriculture to frame its value in ways that would resonate with such gatekeepers, the organizations legitimized urban agriculture for some of its potential benefits rather than others.

These frames would then influence organizational activities, grant applications, and policy deliberations going forward. I find that in all three cities, the main garden organizations came to emphasize an economic framing—employment in Milwaukee, blight removal in Philadelphia, and neighborhood amenity creation in Seattle—while placing relatively less emphasis on potential social and ecological benefits. I demonstrate how the different organizations’ economic frames have succeeded to varying degrees in convincing city officials that garden sites deserve long-term land access, funding, and other forms of public support. At the same time, I note how these frames leave unquestioned the assumption that economic concerns should have primacy over social and ecological ones, setting the stage for future conflicts as the political-economic system has continued to produce inequality and environmental degradation. In discussing Philadelphia, I highlight the role that Soil Generation has played in producing a counter-narrative that reframes the value of urban agriculture as a facet of community self-determination. Soil Generation’s framing subverts economic arguments and calls attention to the need for more just urban land use policy writ large. This chapter reveals how an organizational imperative— gaining and maintaining legitimacy—can inadvertently structure the subsequent framing process that is so important for a social movement’s scope, strength, and success. Thus, I provide new insights into the challenges that community-based organizations are likely to face when they attempt to hybridize into social movement work, and I offer practical lessons for urban agriculture enthusiasts seeking to build and legitimize new garden programs.Chapter 3 considers the organizational environments within each city, particularly the locally shared expectations around governance and policymaking, or “civic conventions,” which have differently constrained or enabled various kinds of garden advocacy, movement organizing, and land-use governance in each locale. In this chapter, I build on the concept of civic conventions theorized by Beamish and others by reconceptualizing civic conventions as a facet of both political and discursive opportunity structure at the urban scale. My analysis of interview and archival data shows that local civic conventions conducive to bottom-up governance in Milwaukee and Seattle have supported the legitimation of urban agriculture as a land use by bringing resident interests to the attention of policymakers and by facilitating the development of garden projects in line with broader public priorities. In contrast, commercial greenhouse supplies in Philadelphia many gardens have remained informal because gardeners see no benefit in engaging with the city government. Local civic conventions hold that the government is often ineffective, and gardeners are also wary of top-down interventions that could threaten their use of the city’s vacant land. Compared to Milwaukee and Seattle, garden informality and suspicion of the government in Philadelphia may have hindered gardener organizing efforts and the public legitimacy of gardens; however, in the last decade, widely shared cynicism about Philadelphia’s city government has provided a discursive opportunity structure in which urban agriculture advocates have effectively framed the loss of gardens in terms of perceived injustice and lack of access to decision-making. This frame, advanced by Soil Generation and its coalition partners, has become a rallying cry for broader mobilization around community control of land and resistance to gentrification.

A similar discursive opportunity structure exists in Seattle, where local civic conventions include a distaste for back-room deals and a narrative regarding the need for ongoing public participation in order to hold city officials accountable. In the 1990s, garden advocates effectively leveraged this narrative to mobilize broad public support for their land use initiative and win the long-term preservation of P-Patches. In this chapter, I highlight the importance of local civic conventions for organizational advocacy and social movement organizing by illustrating how civic conventions in the form of policy infrastructure have created important leverage points and interfaces between community-based organizations and the local government, while civic conventions in the form of widely shared ideas are important to movement formation and mobilization. Chapter 4 considers the organizational environment of local governments as they make decisions about land use policy and budget priorities. Comparing the political-economic conditions of each city, such as the availability of public resources and policy at larger scales of government, I demonstrate how the evolving role of gardens in the urban milieu has interacted with distinct growth strategies and political processes at work in each locale. Across all three case-cities, the globalizing competition to attract capital and “win” at urban growth looms large in city officials’ decision-making. Although the cities vary in their recent histories of “winning” and “losing” the competition for growth, all three cases show how urban growth machine logic and the political-economic pressures on municipalities influence the ways in which urban agriculture has been legitimized as a long-term land use. In Milwaukee and Philadelphia, capital flight has limited the public resources available for social services and urban agriculture investment. Many of the cascading challenges and social maladies are similar for all cities coping with capital flight, but Milwaukee and Philadelphia have diverged in how they construct the role of land in reversing the city’s fortune. In Milwaukee, due to state laws limiting the city’s tools for revenue generation, land is a lifeline that needs to be reserved for badly needed property tax revenue. In Philadelphia, reflecting the narrative advanced by PHS, vacant land is seen as a liability that has burdened the city budget and deterred development. In Seattle, where the local growth coalition has been “winning” in the competition to attract capital and the creative class, land has served as a selling point for the city’s livability. Seattle currently has the most public resources available to invest in its community gardens—but upon close inspection, the benefits still accrue unevenly. In this chapter, I illustrate how pervasively market logic is applied to land use in American cities and how variations in this commodification are connected to the local growth coalition’s status in the global competition for capital. Urban political ecologists have proposed that urban agriculture offers radically transformative potential by nourishing non-capitalist material flows . However, I demonstrate through the varied examples of Milwaukee, Philadelphia, and Seattle that urban agriculture’s radical potential is limited so long as the gardened land remains commodified. Gardens without permanent status are vulnerable to removal in favor of a more economically productive use; furthermore, whether or not gardens are permanently preserved, they may be used as tools to attract high-income residents and new capital investment, displacing low-income residents and perpetuating rather than mitigating urban inequality. In chapter 5, I present a spatial-historical analysis of the accessibility of gardens for marginalized communities in each city. Using a unique dataset developed through my review of historical documents, I demonstrate how the changing locations of gardens reflect the different priorities emphasized by each organization as they pursued legitimacy, and I show how these different priorities led to different outcomes in the proximity of gardens to low-income residents, immigrants, and people of color.