The significance of family type alcoholic family or community family and number of parents who smoke was greatest in the younger age ranges. Effects are measured in changes in logit from baseline. When significant, SNP effects were about 1.0 for having two copies of the risk allele in the recessive genetic models, and the delta ERO effect was about 0.5 per standard deviation. When significant, the parental smoking effect was about 0.2 per smoker, the family type effect ranged from 1.0 to almost 2.0, and the gender effect ranged from about 0.5 to 1.0.In the logistic regression analyses used to investigate the duration of the transition from regular alcohol use to alcohol dependence as a function of the age of onset of alcohol dependence, genotype was not significant in any age range in both linear and quadratic models for duration. In the linear model for duration, modeled as log, Delta ERO values at Fz are signficant in the youngest age range, and both Fz and Cz ERO values are significant in the oldest age range.Duration was significant in the three youngest age ranges. In the quadratic model for duration, modeled as the sum of log and log2 , the Fz and Cz ERO values are significant only in the oldest age range. The effect of duration of drinking was significant in the three youngest age ranges with an overall U-shape in the two youngest age ranges. Since the beta value for the log term is negative and the beta value for the log2 term is positive, the rising part of the U-shape masks the Fz ERO effect in the youngest age range . For the tests of the rapidity of the transition from regular alcohol use to alcohol dependence as a function of the age onset of regular alcohol use, those who become regular alcohol users in the youngest age range were much more likely to become alcohol dependent either in the same age range or the subsequent age range than those who become regular alcohol users in the age ranges 16–17 years or 18–19 years . Viewing this from a slightly different perspective,vertical farm supply the fraction of those who transition from alcohol use to alcohol dependence in less than 2 years in the oldest age range is much smaller than that in the youngest age range.

A Cochran-Armitage trend test of this phenomenon shows a p-value of less than 8 × 10−5 for the hypothesis of no trend. There were age-related trends in the genotypic distributions of those who became alcohol dependent in any of the four age ranges in the illicit drug sub-sample. For the first trend test, of the change of genotypic distribution with age of those who became alcohol dependent at any age, the hypothesis of no trend could be rejected at a 0.003 level for rs978437, rs7800170, and rs1824024, SNPs which were significant for alcohol dependence in the entire population, and at a 0.035 level for rs2061174 and rs2350786. This means that in those who became alcohol dependent, having two copies of the major allele was the prevalent condition for who became alcohol dependent in the earliest age range, while not having two copies of the major allele was the prevalent condition of those who become alcohol dependent in the oldest age range. For the second trend test, of the change of genotypic distribution with time from initiation of alcohol use to time of alcohol dependence of those who began regular alcohol use in the youngest age range and who became alcohol dependent at any age, the hypothesis of no trend could be rejected at a 0.025 level for all of the SNPs. This means that in those who became immediately alcohol dependent, having two copies of the major allele was the prevalent condition, while in those who took the longest to become alcohol dependent, not having two copies of the major allele was the prevalent condition. The results are presented in table 4. This suggests a genetic influence on the rapidity of the transition from regular alcohol use to alcohol dependence among those who become regular alcohol users in the earliest age range. The pattern of significance of the ERO and SNP factors for the onset of regular alcohol use and of alcohol dependence is different between the youngest and oldest age ranges within the entire sample, as is evident in table 2. These differences are primarily the result of differences between the populations of regular alcohol users in the two age ranges. The proportion of the at-risk sample who become regular users of alcohol increased from 15% to 43% between the two age ranges. Biological factors are significant in both the onset of regular alcohol use and of alcohol dependence in the youngest age range. The prevalence of regular drinking in the oldest age range has eliminated the effect of the biological factors in its onset; only the onset of alcohol dependence is affected by biological factors.

In the older age range, since it is likely that much of the onset of alcohol dependence is driven by past drinking, particularly since relatively few of those who become alcohol dependent in the oldest age range have been drinking for a short time, those factors which are significant for regular alcohol use in the youngest age range are significant for alcohol dependence. Furthermore, it is likely that a biologically specific sub-population of the youngest group particularly sensitive to the effects of alcohol has been effectively eliminated from the at risk group in the oldest age range . In the illicit drug use sub-sample in the youngest age range, CHRM2 is a greater factor for the onset of alcohol dependence than in the entire sample. However, EROs are not a factor in the onset of alcohol dependence in this group. The range of ERO values in the illicit drug use sub-sample does not differentiate those who become alcohol dependent from those who do not, although ERO values differentiate the illicit drug sub-sample from their complement in the entire sample. The illicit drug use sample shows greater and more extensive genetic effects than the entire sample, since the result of selecting the illicit drug use sub-sample is to remove those subjects whose alcohol dependence is unlikely to be genetically affected from the analysis. In examining the results of the logistic regression analysis of the transition from regular alcohol use to alcohol dependence in the youngest age range, the U-shaped effect of the duration of drinking suggests the presence of two distinct factors, one a susceptibility to rapidly become dependent subsequent to the onset of regular alcohol use and the other a gradual effect of continued alcohol consumption. The masking of the ERO effect by the rising component of the duration factor suggests that ERO is associated with a long term behavior pattern involving substance abuse. The absence of a genotypic effect is the result of including all those who become alcohol dependent in the analysis, not just those in the genetically more vulnerable, as can be observed by comparing the under 16 results between the regular alcohol user group and the illicit drug user group. In summary, for the youngest age range the pattern of significance of the ERO and SNP phenotypes for the onset of regular alcohol use and of alcohol dependence,vertical farm tray as well as the pattern of significance in the transition from alcohol use to alcohol dependence suggests that delta ERO value indexes an element of propensity to use drugs to excess, while the CHRM2 SNPs index an age related effect of alcohol consumption on the brain with the behavioral outcome of dependence, as we explain below. We view the age-varying genotypic effect of the CHRM2 SNPs as an instance of a gene environment interaction.

In our case the immediate genotypic effects are upon the activation level of the type 2 muscarinic receptors and the environment is the neuroanatomic and neurophysiological context in which the action of the muscarinic receptors is taking place. This environment undergoes significant changes as the brain develops from the early teens into the early twenties, as we have noted above. In the transition from alcohol non-use to regular use of alcohol to alcohol dependence, we note that alcohol consumption has significant effects on the development of addiction in adolescent animals and humans . The cholinergic M2 receptor gene belongs to a family of muscarinic acetylcholine G-protein coupled receptors with five known subtypes . The M2 receptors in the mesolimbic dopaminergic system play a significant role in modulating the level of dopamine release . This has a important effect in governing the reward system , including modulating the effects of alcohol on it . M2 receptors also modulate synaptic transmission in cortical circuits affecting the pyramidal neurons . It is not possible to determine the precise nature of the interaction between the genotypic effect on the cholinergic M2 receptors and the age-varying neuroanatomic/ neurophysiologcial environment given the data at our disposal. Given the age-related patterns of genotypic action we have described above, it is possible that the effect of alcohol consumption on the brain varies with the genotype of the cholinergic M2 receptors and the age of onset of regular drinking. Specifically, when alcohol is consumed regularly in the youngest age range, perhaps better described as a particular stage in brain maturation centered in this age range, the addiction producing effects on those who have two copies of the major allele are accelerated compared to those who do not, leading to rapid transition from regular alcohol use to alcohol dependence.Those without two copies of the major allele may take longer to manifest the effects of alcohol use. As the age of the initiation of alcohol use increases, it appears that the cumulative risk for alcohol dependence when carried into the adult years is greater in those without two copies of the major allele than in those with two copies. We draw this last conclusion on the basis of the trend tests on our own data and the results of the studies of Wang et al. and Dick et al. . In those who become regular users of alcohol under the age of 16, a majority of those who became alcohol dependent within two years had the risk genotype; the majority of those who become alcohol dependent four years or more after their onset of regular drinking did not have the initial risk genotype. A contributing factor to the age specificity of the effect of the CHRM2 SNPs could be a frailty effect. The frailty effect would play a role if there were relatively easy access to alcohol in the youngest age range, at least for those most at risk. Among those who have the major alleles, those who are genetically most vulnerable become alcohol dependent rapidly, leaving only those who have some protective factor. Thus risk for those with the major alleles will decrease with age, since those without the protective factors will have become alcohol dependent, leaving primarily those with protective factors at potential risk. We also note that if the illicit drug user population had easier access to alcohol than the entire population as a whole, the greater genetic effects seen in the illicit drug usersub-sample might in part be the result of a gene-environment interaction, akin to those described in Dick and Kendler , in which looser social controls over behavior accentuate genetic effects. Since 80% of the illicit drug use sub-sample are from COGA rather than community families, this is a plausible hypothesis. The specific environment of the most vulnerable group is more likely to accentuate genetic effects, rather than to diminish them.We found that SNPs reported to be significant in adults were significant in adolescents in this sample, particularly for those in the youngest age ranges, and for those who had ever used an illicit drug. However, in our results, the major allele was the risk allele, while in the results of Wang et al. and consequently of Dick et al. , the minor allele was the risk allele.