Tag Archives: vertical farming pros and cons

Farms rely heavily on donated land and volunteer and citizen labor

While there are limitations in our ability to generalize findings to the East Bay urban farming landscape as a whole due to the relatively small sample size, we obtained a fairly representative sample of the diversity of farm types in the East Bay based on our typology of the original 120 farm types . Survey questions fell into nine categories: 1) Background Info, 2) Farm Description, 3) Operating Expenses and Revenues, 4) Land Access and Tenure, 5) Production and Soil Health, 6) Distribution, 7) “Waste” and Compost, 8) Food Access, and 9) Training, Communications, and Follow Up. There were a few open-ended questions allowing farmers to express what they saw as the three largest challenges facing urban agriculture operations in the area, and policy-relevant suggestions for securing spaces for urban farms and increasing community food security. In addition, we interviewed five urban farmers to deepen our understanding of the social, political, economic, and ecological constraints under which their farms operate. These farmers are particularly involved in networking efforts to strengthen urban farm viability in the East Bay. Four out of five represent locally prominent non-profit farms and one subject represents an alternative cooperatively-run urban farm; three interview subjects are women and two are men. Our study complied with UC Berkeley’s Institutional Review Board protocol for the protection of human subjects and all participants gave consent for participation.Farmers reported diversified distribution methods including volunteers harvesting and taking food home , on-site consumption , on-site farm stand distribution, CSA boxes at pick up sites,vertical cannabis and volunteers delivering produce directly to distribution sites . Some gleaning and second harvesting occurs at urban farms and gardens with potential for growth given reported “unharvested” and “wasted” food percentages.

Backyard produce is also exchanged through crop swaps and neighborhood food boxes . Eight operations reported having access to a refrigerated truck for food deliveries, and two are willing to share their truck with other farmers. There is no universally used or city-organized process for distributing produce off of urban farms and into the community, yet there exists great interest in aggregating produce or distribution channels , an unrealized goal of urban farmers in the East Bay. All of the food system stakeholders involved in our study are working towards transformative food system change, focused on increasing equity, food security, and access to healthy, locally sourced food. See Box 1 for a description of one of the non-farmer stakeholders engaged in the food recovery and distribution system, who has recently established an aggregation hub to serve as a network for reducing food waste and channeling excess food in the urban community to those who are food insecure.Farmers in our study stressed the importance of producing non-food related values on their farms, including education and community building. One farmer in particular emphasized their organization’s mission of growing urban farmers growing food,” or teaching other people how to grow a portion of their food basket, thus unlocking food sovereignty and food literacy while increasing healthy food access. Another respondent reported that their farm is “highly desirable for adults with special needs that need a safe place to be outside,” echoing respondents who point out the intimate connection between food and health . Farms frequently reported hosting educational and community-building workshops, cooking and food processing demonstrations, harvest festivals, and other open-to-the-public community events enhancing the resilience and connectivity of people, communities and ecosystems. Social networks emerged as an important theme for enabling the establishment of urban farms , and sustaining operations through social connections between urban farmers and other food justice and health advocates. Farmers identified three primary challenges: revenue, land, and labor inputs.

Half of all respondents reported farm earnings of $1,500 annually or less, and all four operations receiving over $250,000 in annual revenue are well-funded non-profit operations . Regardless of for-profit or non-profit status, most farms reported multiple sources of revenue as important to their continued operation , with an average of 3 revenue streams per farm. All non-profit farms reported multiple revenue streams except for three, who were sustained entirely by either board donations, membership fees , and grants. The most important revenue sources for non-profits include grants, grassroots fundraising, and unsolicited donations rather than sales. In addition to these monetary sources, all farms reported receiving substantial non-monetary support , which adds to the precarity of operations when these informal support channels disappear.Land tenure arrangements range from land accessed without payment through contracts with City or School District officials, to arrangements where a token fee is paid , to more formal leasing arrangements at the utility-owned Sunol Ag Park, where land tenants pay $1000/acre/year for their plots, ranging from 1-3 acres. Only five of the respondents owned their land , representing a mix of for-profit and nonprofit operations . Challenges around land access, security, and tenure were the most frequently occurring theme in the survey long response and interview analysis process, including consensus that land access is the largest barrier to scaling UA in the East Bay. The cost of labor, and relatedly, access to capital and grant funding to pay living wage salaries, were also extremely significant challenges identified by survey respondents. The majority of respondents stated that most of their labor is volunteer rather than paid, with nonprofit respondents reporting this more frequently than for profit enterprises . The maximum number of paid staff at any operation is 20 , while the average is 4. Many farms reported the desire to be able to hire and pay workers more, but not having sufficient revenue to accomplish that goal. Annual volunteer labor participants on farms ranged from 0 to 1542 with an average of 97 volunteers, representing a significant public interest in participating in local food production. Not surprisingly, amount of paid labor and total farm income are positively correlated . However, volunteer labor is also positively but more moderately correlated with total farm income .The farmers in our study acknowledged many challenges facing urban agriculture, stemming both from the high economic costs of production and land rents, and insufficient monetary returns from produce sales.

They also framed these challenges through a food justice lens, arguing that the current political economy does not fully compensate farmers for the social-ecological services provided from their farms. Farmers articulated many solutions that could improve the viability of their farm operations including: conversion of city parks into food producing gardens with paid staff, government and institutional procurement goals for urban produced foods, municipal investment in cooperatives or other community based food production , and establishment of aggregation hubs and distribution infrastructure.Our survey results describe a highly diversified East Bay Agroecosystem comprising urban farmers and other food system stakeholders that are growing food as well as food literacy, civic engagement, connectivity, and community. Applying an agroecological lens to interpret our findings of East Bay urban agriculture operations reveals the many agoecological practices farms have long been engaged in, as well as the important distinctions of UAE that still need to be explored, and specific threats to agroecology in urban areas. Pimbert suggests that “agroecology’s focus on whole food systems invites urban producers to think beyond their garden plots and consider broader issues such as citizens’ access to food within urban municipalities and the governance of food systems.” We argue that applying an agroecological lens to the urban context also invites researchers and urban planners and policymakers to think beyond garden plots and singular benefits of food production, to consider these sites as part of a larger agro-ecosystem with synergistic social,vertical farming pros and cons cultural and ecological dimensions. We reference the 10 elements of agroecology to illustrate the dynamics of how these elements manifest in practice in this urban context.All of the farms in our survey follow agroecological production practices which include a focus on building soil health through, most commonly, cover cropping, compost application, and no-till practices. These practices produce synergistic effects of adding fertility to the soil through organic matter amendments and boosting water holding capacity. Soil building practices are a response to the impetus to remediate toxins present in urban soils , a prerequisite to intensive cultivation and unique consideration of the urban farm environment. Overall, production practices on our urban farms seek to conserve, protect and enhance natural resources.

Our survey respondents described numerous strategies for enabling diversified, intensive production of fruits, vegetables, and other agricultural products. These strategies span both short and long-term, from planting in raised beds with imported soil, to building soil health in situ via heavy applications of compost, manure, and cover crops for several years leading up to vegetable crop production. There is a growing interest in using no-till practices, which are among the suite of practices associated with “carbon farming” for enhancing soil carbon sequestration . This illustrates a synergistic opportunity for urban food policy and urban climate policy, showing where urban food production and city Climate Action Plans 4 can converge and generate mutual support . Farmers are also engaged in innovative resource recycling and resource use efficiency and other strategies to enhance resilience such as installing rainwater catchment systems in concert with swales and soil health practices to optimize use of this scarce resource. Farms are planting native flowers and shrubs to attract beneficial insects, rather than purchasing chemical inputs for pest management. From a city planning perspective, the impetus to remediate storm water overflows and maintain corridors for essential pollinators are two priorities that can be met through incentivizing and planning spaces for UAE.East Bay urban farms reflect multiple scales and forms of diversity including agrobiodiversity, organizational and participant diversity, diversified sources of capital, labor and land arrangements, as well as diversified modes of exchange. Diversity among operations technically doing the same thing- growing food in cities- signals the fluid, flexible, peripheral, and at times revolutionary nature of urban food production spaces, which may conflict with orresist the institutional, political-economic status quo . Urban farms rely on diverse revenue streams from their diversity of activities beyond sale of produce. These activities, including educational services and community events, are important to elevate in policy conversations. Valuing and therefore protecting urban food production spaces requires thinking differently about them in a context like the San Francisco Bay Area. One stakeholder suggested considering urban farms as museums, providing essential cultural and educational offerings to city residents . The quality of the food and the value of the education, health, and community building, are strong arguments for including urban farms in an urban-agroecological framework for city planning and efforts to improve CFS. The diversity of land access agreements and labor sources used by urban farmers in the East Bay underscores equity considerations in urban agroecological transitions. Even 50% of the for-profit enterprises reported relying on volunteer labor, speaking to both the precarious economics of running an economically viable for-profit food production business in the city, and the interest among young people and aspiring farmers in gaining agroecological cultivation skills through arrangements where they donate their labor free of charge. Volunteer labor substitutes for revenue to a certain degree, allowing farms to exist and distribute food informally without needing to generate much revenue or provide many jobs. In the UA literature, reliance on volunteer labor comes under criticism for being a product of the “neoliberal city,” where responsibility for action falls to the individual rather than the state, and the equity concerns around who is able to volunteer their time are problematized . By reporting the common use of volunteers on East Bay urban farms, we do not seek to promote or valorize this practice, but rather recognize it as a necessary interim step occurring in our study context in the absence of dramatic local government intervention or radical reforms to address community food insecurity: those who are willing and able are participating through civic engagement in urban farms to produce, harvest and distribute healthy food to those in need. Many volunteers are retired or recent graduates, seeking opportunities to contribute meaningfully to their communities. The volunteers we have communicated with generally report positive experiences and enjoyment from their time digging in the soil.

Families express a range of emotional reactions to this information

The results indicated that C6- and C7-isoprenols have comparable RON boosting effects to isopentenols, making these two chemicals potential blendstocks for gasoline blends. We previously constructed the homoterpene biosynthesis platform as a proof of concept that introduces terpene structural diversity at the precursor stage. Here we further optimized this platform towards practical application. The most significant change is the upstream pathway to the key intermediate, 3- ketovaleryl-CoA. Like the natural LMVA pathways, our previous pathway starts from propionyl-CoA, condensed by a thiolase into 3-ketovaleryl-CoA. Two points led us to consider an alternative pathway to 3-ketovaleryl-CoA. First, thermodynamic analysis indicated the condensation reaction catalyzed by thiolase is endergonic with a positive Gibbs free energy change , suggesting the thiolase catalyzed condensation reaction is thermodynamically unfavorable. This calculation is consistent with the finding that PhaA homologs catalyze the degradation reaction better than the condensation reaction . Second, to our knowledge, almost all the reported thiolases that convert propionyl-CoA and acetyl-CoA into 3-ketovaleryl-CoA also convert two molecules of acetyl-CoA into acetoacetyl-CoA. Our later experiment using butyric acid as substrate in the beta-oxidation LMVA pathway suggests the LMVA pathway readily accepts acetoacetyl-CoA into IPP . Considering these two points and the relatively high concentration of acetyl-CoA in E. coli , we reasoned that it would be difficult for the thiolase LMVA pathway to make HIPP over IPP, complicating C16, C17 and C18 terpene biosynthesis. Instead, the beta-oxidation pathway is a more specific way to produce isopentenyl pyrophosphate analogs. Although background IPP production remains, either via the native MEP pathway or from endogenous acetoacetyl-CoA transformed by the LMVA pathway,curing marijuana the ratio of HIPP/IPP production is significantly improved, as the molar ratio of C6-isoprenol:isoprenol is over 60 in the production run using the E. coli BL21 ΔatoB host with 1 g/L valeric acid feeding.

The high production of HIPP and its dominant content in the isopentenyl pyrophosphate analog pool will benefit future homoterpene biosynthesis efforts. Using isoprenol analogs as the final product, we successfully optimized the flux to HIPP in the homoterpene biosynthesis platform. The enzymes after the LMVA pathway leading to complex terpenes are more challenging to optimize because of their elusive enzymology and unusual substrates. Following HIPP production, an IDI is supposed to isomerize HIPP to HDMAPP. We could not detect the corresponding alcohol of HDMAPP, C6-prenol, in the E. coli production run with the expression of NudB and the thiolase LMVA pathway containing the IDI from Bombyx mori. While this IDI was confirmed in vitro to transform HIPP to HDMAPP specifically , the absence of C6-prenol in the production run suggests that this IDI does not work well in E. coli, or the hydrolyzed product of HDMAPP, HDMAP, is not well accepted by the E. coli endogenous phosphatases. Incorporating this IDI into the optimized beta-oxidation LMVA pathway may increase HDMAPP production by increasing substrate supply. Other Lepidopteran IDIs are also candidates for enzyme screening of this step. At the same time, it is noteworthy that the regiospecificity of some Lepidopteran IDIs are low, because they transform HIPP to not only HDMAPP but also the -isomer of HDMAPP and isomers with a γ-δ double bond . After the isomerization, ideally, one molecule of HDMAPP is supposed to condense with different molecules of HIPP into homo-GPP , homo-FPP , and homoGGPP . To our knowledge, all the characterized FPPSs that produce homo-FPP also produce FPP, with varied substrate preferences. This substrate promiscuity could explain why our previous work only produced C16 homosesquiterpenes as low, or non-existent HDMAPP levels may hamper homo-FPP analog production. The overwhelming production of HIPP to IPP in the optimized platform here may increase the HIPP incorporation to produce more C16, C17, and even C18 FPPs. For other prenyl diphosphates, reported point mutations in prenyltransferases that change the product profiles could be applied on the lepidopteran FPPS to produce homo-GPP . Also, the structural basis of substrate preference for HIPP/HDMAPP derived prenyl diphosphates has been analyzed, the results of which are proposed to direct the engineering of non-lepidopteran prenyltransferases to accept HIPP/HDMAPP . Finally, terpene synthases cyclized the homo prenyl pyrophosphates to terpene scaffolds.

This step is the most challenging due to the lack of natural enzymes using homo prenyl pyrophosphates as the substrates. Future studies will focus on using rational design and directed evolution to alter the substrate specificity of canonical terpene synthases. Introducing extra carbon in the terpenes can significantly change their properties, exemplified by the optimized fuel properties of isoprenol biofuels. With comparable RON boosting effects and energy densities to isopentenols, C6- and C7-isoprenols have decreased water solubilities, making them better ingredients for fuel blends . In particular, C6-isoprenol derives fromvaleric acid, a key intermediate in the valerate biofuel platform . Numerous chemical reactions/processes have been developed to transform lignocellulose to valeric acid via levulinic acid , making our pathway promising to produce C6- isoprenol as a next-generation biofuel. Besides the simple terpenes we produce here, another example is -germacrene D, whose analog with two extra carbons, -14,15-dimethylgermacrene D shows a reversal in insect behavioral activity . Addressing those challenges in the homoterpene biosynthesis will enable the efficient production of various terpene analogs, leading to more diversified structures in the chemical portfolio for downstream applications. Proper adherence to combination antiretroviral medication therapy is critical for improving health outcomes in HIV . However, it is estimated that less than 40% of HIV+ individuals are retained in long-term healthcare management and only about one-quarter are virally suppressed in the U.S. . Further, approximately half of individuals prescribed antiretroviral medications do not fully adhere to their regimen . Suboptimal cART adherence is associated with increased viremia, immune suppression, increased risk of HIV transmission, and mortality . Numerous factors appear to be contributing to suboptimal cART adherence, including psychiatric comorbidities , lack of social support, severity of antiretroviral side effects, beliefs about self-efficacy and neurocognitive dysfunction .

With regard to the latter, HIV-associated neurocognitive deficits and cART non-adherence appear to have a cyclical relationship; that is, neurocognitive deficits can interfere with medication taking behaviors , which in turn can accelerate disease progression and further impair neurocognitive functioning . The historic complexity of combination antiretroviral therapies may also impose an undue burden on cognitive abilities necessary for adherence . The medications comprising a cART regimen may involve differing dosages and/or administration schedules, which could increase the risk of errors that negatively impact overall adherence, particularly among individuals with neurocognitive difficulties. Although the pill burden of cART is on a downward trajectory, polypharmacy remains common and these challenges are important given that cART is the gold standard for HIV treatment in the United States. Additionally, the prevalence of HIVassociated neurocognitive disorders may have even risen slightly in the cART era, with approximately 50% of the population affected . The elevated rates of HAND and domain-based impairment may be due to increasing lifespans resultant from high cART efficacy,dry racks for weed leading to longer exposure to both the presence of virus as well as potentially toxic long-term effects of the drug regimens, combined with comorbid processes such as cognitive aging . While HIV-associated neurocognitive deficits can be observed in a variety of neurocognitive domains, the prevalence of impairment in higher-order neurocognitive abilities, such as episodic memory in particular, are higher . In this study we therefore focus our attention on the role of episodic memory deficits in cART non-adherence. HIV-associated neural injury in frontal and temporal systems affect multiple aspects of episodic memory , including traditional retrospective memory for word lists, designs, and passages, as well as prospective, source, and temporal order memory . The profile of HIV-associated episodic memory deficits is heterogeneous , with prior studies showing evidence that numerous specific memory processes may be disrupted, including learning/acquisition, storage/consolidation, and retrieval . Anywhere from 20 to 40% of HIV+ individuals exhibit a primary retrieval profile of memory deficits consistent with injury to fronto-striato-thalamo-cortical circuits. This retrieval profile is characterized by difficulties in bringing previously stored information into conscious awareness and is evidenced by deficits in free recall of information that are ameliorated when structured retrieval cues are provided . A retrieval profile, which is sometimes referred to as a mixed encoding/retrieval profile, can suggest that an individual only partially encodes the target information. This is because stimuli that were processed only in part are difficult to spontaneously recall in their entirety, but may be accurately recognized when presented.

HIV-associated deficits in learning and memory have been linked to poorer everyday functioning, including medication management skills and cART non-adherence. Deficits in acquisition and delayed free recall as a global composite index have been consistently associated with poor performance on laboratory-based medication management tasks across varying types of stimuli . Among the two studies that we found examining specific memory components in the context of adherence, measures of delayed free recall were more consistently associated with medication adherence , whereas indices of initial learning show more variable associations. Yet the specificity of such delayed recall deficits is difficult to determine, as they may be a consequence of problems with encoding, forgetting, and/or retrieval. Thus, while learning and memory are consistently associated with medication management in the laboratory and daily life, little is known about the specific profile that may be driving these relationships. Identification of such profiles at the levels of both group data and individual participants is important in order to enhance the clinical identification of persons at risk for non-adherence and develop tailored compensatory mnemonic approaches to improve adherence. To that end, Wright and colleagues applied the item specific deficit approach , an item analytic method, to study the association between cART adherence and word list encoding, consolidation, and retrieval as measured by the California Verbal Learning Test in 75 HIV+ participants. The ISDA indices were developed as a novel method for categorizing encoding, consolidation and retrieval deficits. The ISDA encoding index is constructed by summing the number of items recalled in less than three of the five learning trials, the ISDA consolidation index reflects the number of items recalled during the learning trials but not recalled again during any of the recall trials , and retrieval deficits are indexed by the number of learned items recalled inconsistently across short- and long-delay trials. To control for potential group differences in learning, the consolidation and retrieval indices are each divided by the total words recalled at least once during the learning trials. When using the ISDA Wright and colleagues found that, compared to healthy adults, HIV+ individuals demonstrated poorer performance on the CVLT regardless of their level of antiretroviral adherence. Additionally, while both HIV+ groups demonstrated similar ISDA encoding deficits, only non-adherers demonstrated ISDA retrieval deficits compared to HIV− participants. Additionally, retrieval abilities, as measured by the ISDA indices, accounted for a greater proportion of variance in long-delay free recall performance for poor adherers than for good adherers. While the study conducted by Wright and colleagues provided initial evidence of an association between retrieval deficits and sub-optimal adherence in HIV, no studies have evaluated the association between sub-optimal adherence and traditional list learning profiles that also incorporate retention and recognition performance as is commonly done in clinical research and practice. At the group level, such profile-based approaches have a long tradition of utility in distinguishing between different neuropsychological disorders. For example, retrieval deficits in the context of significantly improved recognition are commonly associated with “subcortical” dementias . “Cortical” dementias , on the other hand, are sometimes differentiated by rapid forgetting, minimal improvement on recognition, and high rates of cued recall intrusions . Our approach here is to employ those same cognitive psychology approaches of learning and memory profile distinctions to better understand the cognitive architecture of non-adherence. To further enhance the clinical relevance of this group-level analytic approach, we also propose to classify individuals’ profiles as reflective of problems with encoding versus retrieval using an established data-based algorithm that has shown utility differentiating traditionally cortical versus sub-cortical diseases . This algorithm-based individual profile approach has proven to be a worthwhile complement to group-based analyses by allowing for clinically-relevant classification accuracy data and demonstrating the heterogeneity of profiles within these traditionally “cortical” and “sub-cortical” groups.